Apc cy7 anti mouse cd45 antibody

Manufactured by BioLegend

The APC-Cy7 anti-mouse CD45 antibody is a fluorescently-conjugated monoclonal antibody that specifically binds to the CD45 antigen expressed on mouse leukocytes. It can be used in flow cytometry for the identification and enumeration of mouse immune cell populations.

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Lab products found in correlation

Fitc anti mouse cd3 antibody, by BioLegend (1 mentions) Fitc anti human cd3 antibody, by BioLegend (1 mentions)

Close spelling variants of this product

APC/Cy7-conjugated anti-mouse CD45 antibody APC/Cy7-conjugated rat anti-mouse CD45 antibody APC/Cy7 anti-mouse CD45 APC anti-mouse CD45 antibody Anti-CD45-APC-Cy7 CD45-APC-Cy7 APC anti-mouse CD45 Anti-mouse CD45 antibody Anti-CD45-APC Anti-mouse CD45

2 protocols using apc cy7 anti mouse cd45 antibody

Phenotypic Analysis of pNKs and DICs

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pNKs cocultured with DSCs or decidual HESCs were collected, and the expression of CD45, CD3, CD56 and CD16 was analyzed by flow cytometry assays. Briefly, the collected pNKs were washed twice with PBS and stained with PerCP anti-human CD45 antibody (Biolegend, San Diego, CA, USA), FITC anti-human CD3 antibody (Biolegend), BV421 anti-human CD56 antibody (Biolegend) and APC-Cy7 anti-human CD16 antibody (Biolegend) diluted in PBS with 2% FBS at 4 °C for 30 min. pNKs were then washed twice and analyzed by flow cytometry assays.
DICs isolated from decidual tissue of mice treated with DMSO or LDN57444 were collected, and the expression of CD45, CD3 and NK1.1 was analyzed by flow cytometry assays. The procedure was described above, and the antibodies used were APC-Cy7 anti-mouse CD45 antibody (Biolegend), FITC anti-mouse CD3 antibody (Biolegend) and BV421 anti-mouse NK1.1 antibody (Biolegend).

Zhang J., Xue M., Huang J., He S., Zhu L., Zhao X., Wang B., Jiang T., Zhang Y., Miao C, & Zhou G. (2024). Deficiency of UCHL1 results in insufficient decidualization accompanied by impaired dNK modulation and eventually miscarriage. Journal of Translational Medicine, 22, 478.

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Flow Cytometric Analysis of MSC Phenotype

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For analysis of mesenchymal stem cell (MSC) phenotype [31 (link)], BMSC and SMSCs were assayed for cell surface markers: CD45, CD44, CD29. 1 × 10⁶ BMSC and SMSC were seeded onto a 10 cm cell culture plastic dish and cultured for 5 days in growth media. Cells were incubated with APC/Cy7 anti-mouse CD45 antibody (cat. 103116, Biolegend, San Diego, CA), APC anti-mouse CD44 antibody (cat. 103012, Biolegend), FITC anti-mouse CD29 antibody (cat. 102206, Biolegend) for 30 min at 4C. 4’,6-diamidino-2-phenylindole (DAPI, cat. NBP2–31156, NOVUSBIO, Centennial, CO) was used for determining cell viability. The cell phenotypes were evaluated by FACS analysis for DAPI−;CD45−;CD44+;CD29+ (FACSAria Ilu Flow Cytometer, Becton Dickinson, Mountain View, CA).

Swanson W.B., Omi M., Zhang Z., Nam H.K., Jung Y., Wang G., Ma P.X., Hatch N.E, & Mishina Y. (2021). Macropore Design of Tissue Engineering Scaffolds Regulates Mesenchymal Stem Cell Differentiation Fate. Biomaterials, 272, 120769.

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