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Nf light

Manufactured by Quanterix
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The NF-Light is a lab equipment product from Quanterix. It is a sensitive instrument designed to measure and quantify specific protein biomarkers in biological samples.

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Lab products found in correlation

Simoa hd x analyzer, by Quanterix (2 mentions) Hd x analyzer, by Quanterix (1 mentions) Simoa sr x, by Quanterix (1 mentions) Simoa hd 1 analyzer, by Quanterix (1 mentions) Explore 1536, by Olink (1 mentions) Hd 1 single molecule array simoa, by Quanterix (1 mentions)

Close spelling variants of this product

NF-Light Advantage Kit Simoa NF-light assay Advantage NF-Light Singleplex-Kit Simoa Human NF-light Advantage Kit NF-Light kit Simoa® NF-light™ Advantage (SR-X) Kit Simoa NF-Light Advantage Kit NF-light Advantage (SR-X) kit Human NF-light Advantage kit NF-light” assay kits

11 protocols using nf light

1

Serum NFL as a COVID-19 biomarker

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The goal of this study was to investigate serum NFL as a biomarker of neuroaxonal injury in patients with COVID-19. NFL was measured using the NF-Light digital immunoassay from Quanterix. Our primary analyses were to determine whether serum NFL is elevated in hospitalized patients with COVID-19 and whether NFL associates with clinical outcomes and treatment type. We included 142 patients with COVID-19 (no randomization) for whom serum (488 samples) was collected cross-sectionally or longitudinally during hospitalization. Our study also included serum from 55 healthy controls. NFL was measured in a blinded manner. Sample sizes were based on what was available when the study was initiated and not on sample size calculations. Biological samples were obtained when residual blood was available from patients with approval by the ethics committee.
Prudencio M., Erben Y., Marquez C.P., Jansen-West K.R., Franco-Mesa C., Heckman M.G., White L.J., Dunmore J.A., Cook C.N., Lilley M.T., Song Y., Harlow C.F., Oskarsson B., Nicholson K.A., Wszolek Z.K., Hickson L.J., O’Horo J.C., Hoyne J.B., Gendron T.F., Meschia J.F, & Petrucelli L. (2021). Serum neurofilament light protein correlates with unfavorable clinical outcomes in hospitalized patients with COVID-19. Science Translational Medicine, 13(602), eabi7643.
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2

Quantification of serum neurofilament light

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Non-fasting blood samples were collected from each consenting participant immediately after the interview. Samples were processed directly in the study center within 2 h. After centrifugation, serum aliquots (370 and 500 μl) were prepared for long-term storage at – 80 °C. sNfL was quantified with a commercially available kit (NF-Light, Quanterix) that was applied on the single molecule array HD-X analyzer (Quanterix, Lexington, MA, USA), as previously described. Samples were run in duplicate by board-certified technicians blinded to clinical information. The coefficients of variation for all samples reported were < 15%.
Hermesdorf M., Wulms N., Maceski A., Leppert D., Benkert P., Wiendl H., Kuhle J, & Berger K. (2023). Serum neurofilament light and white matter characteristics in the general population: a longitudinal analysis. GeroScience, 46(1), 463-472.
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3

Plasma NfL Measurement Protocol

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After blood collection, all samples were transferred to each local laboratory where they were centrifuged, aliquoted and frozen at −80°C after extraction, following international recommendations. APOE genotype was determined at each centre. Plasma samples were shipped in dry ice to the laboratory in Hospital Sant Pau (Barcelona, Spain) where they were stored at −80°C until analysis.
Levels of plasma NfL were centrally measured in Hospital Sant Pau using the ultrasensitive equipment Simoa SR-X™ (Quanterix). All samples were measured in duplicate, and within one round of experiments between August and September 2019 using commercially available kits (NF-light™, Quanterix). Intra- and inter-assay coefficients of variation were 3·4% and 16·7%, respectively. Baseline and longitudinal samples obtained from each participant were measured side by side in the same run to avoid the effect of run-to-run variability. All analyses were performed by one technician, who was blind to clinical diagnosis. A subset of samples from this study had been previously analysed in a Simoa HD-1™ equipment (Montpellier, France). There was a high correlation between both assays (R2=0·94, see Supplementary materialand supplementary figure 4).
Carmona-Iragui M., Alcolea D., Barroeta I., Videla L., Muñoz L., Van Pelt K.L., Schmitt F.A., Lightner D.D., Koehl L.M., Jicha G., Sacco S., Mircher C., Pape S.E., Hithersay R., Clare I.C., Holland A.J., Nübling G., Levin J., Zaman S., Strydom A., Rebillat A.S., Head E., Blesa R., Lleó A, & Fortea J. (2021). Diagnostic and prognostic performance and longitudinal changes in plasma neurofilament light chain concentrations in adults with Down syndrome: a cohort study. The Lancet. Neurology, 20(8), 605-614.
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4

Quantifying Neurofilament Light in Biological Fluids

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Plasma and CSF NfL concentrations were measured in duplicates using the 1-plex single molecule array (Simoa) kit (NF-Light®, Quanterix) on a Simoa HD-1 Analyzer (Quanterix)17 (link) according to the manufacturer’s instructions. All blood and CSF samples from collaborating centres were tested in the UCL laboratory, using one batch of reagents (lot 501769). All NfL values were within the linear range of the assay. For plasma, the mean intra-assay coefficient of variation (CV) of duplicate determinations for concentration was 4.5%. In the CSF, the mean intra-assay CV was 3.8%.
Chelban V., Nikram E., Perez-Soriano A., Wilke C., Foubert-Samier A., Vijiaratnam N., Guo T., Jabbari E., Olufodun S., Gonzalez M., Senkevich K., Laurens B., Péran P., Rascol O., Le Traon A.P., Todd E.G., Costantini A.A., Alikhwan S., Tariq A., Ng B.L., Muñoz E., Painous C., Compta Y., Junque C., Segura B., Zhelcheska K., Wellington H., Schöls L., Jaunmuktane Z., Kobylecki C., Church A., Hu M.T., Rowe J.B., Leigh P.N., Massey L., Burn D.J., Pavese N., Foltynie T., Pchelina S., Wood N., Heslegrave A.J., Zetterberg H., Bocchetta M., Rohrer J.D., Marti M.J., Synofzik M., Morris H.R., Meissner W.G, & Houlden H. (2022). Neurofilament light levels predict clinical progression and death in multiple system atrophy. Brain, 145(12), 4398-4408.
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5

Quantifying NfL and IL-6 in Serum

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Serum levels of neurofilament light chain (NfL) and interleukin-6 (IL-6) were measured using a single-molecule array assay (SIMOA) (Quanterix™, NF-light and IL-6 ®Advantage Kit, Billerica, MA, USA). The lower limit of quantification (LLOQ) for NfL was of 0.343 pg/ml, with a dynamic range from 0 to 1800pg/ml. For IL-6 the LLOQ was 0.0103 pg/ml with a dynamic range from 0 to 120 pg/ml. Inter- and intra-assay coefficients of variations (CVs) for assays are shown in appendix, Table S1.
Olsson A., Gustavsen S., Nguyen T.D., Nyman M., Langkilde A.R., Hansen T.H., Sellebjerg F., Oturai A.B, & Bach Søndergaard H. (2021). Serum Short-Chain Fatty Acids and Associations With Inflammation in Newly Diagnosed Patients With Multiple Sclerosis and Healthy Controls. Frontiers in Immunology, 12, 661493.
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6

Neurofilament Light in Mouse CSF

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NFL concentrations in the mouse CSF were measured in duplicate on a Simoa HD-X analyzer using an NF-Light digital immunoassay (Quanterix #103186) according to the manufacturer’s instructions. Briefly, samples were thawed and cleared by centrifugation at 10,000 x g for 5 min. Samples were diluted 1:25 at the bench, transferred to a 96-well plate, and ran in duplicate using a 4x instrument dilution for a final dilution of 1:100. NFL concentrations were interpolated from the calibration curve using a 1/y2 weighted four-parameter logistic curve fit.
Jansen-West K., Todd T.W., Daughrity L.M., Yue M., Tong J., Carlomagno Y., Del Rosso G., Kurti A., Jones C.Y., Dunmore J.A., Castanedes-Casey M., Dickson D.W., Wszolek Z.K., Fryer J.D., Petrucelli L, & Prudencio M. (2022). Plasma PolyQ-ATXN3 Levels Associate With Cerebellar Degeneration and Behavioral Abnormalities in a New AAV-Based SCA3 Mouse Model. Frontiers in Cell and Developmental Biology, 10, 863089.
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7

Quantifying Serum Neurofilament Light

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Non-fasting blood samples were taken, centrifuged and stored at − 80 °C within two hours. Later, sNfL was quantified by blinded, board-certified technicians using a commercially available kit (NF-Light, Quanterix) and a single molecule array (SIMOA) HD-X analyzer (Quanterix, Lexington, MA, USA). The coefficients of variation were below 15% for all samples. Due to the right-skewed distribution of sNfL, the variable was log10-transformed prior to the analyses.
Hermesdorf M., Leppert D., Maceski A., Benkert P., Wellmann J., Wiendl H., Kuhle J, & Berger K. (2022). Longitudinal analyses of serum neurofilament light and associations with obesity indices and bioelectrical impedance parameters. Scientific Reports, 12, 15863.
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8

Serum NfL Quantification Using Simoa Assay

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Serum NfL levels were measured using the Simoa® assay (NF-LIGHT, Quanterix) performed at the University of Basel, Switzerland. Assay techniques and principles have been previously described.9 (link)
Yeo T., Probert F., Sealey M., Saldana L., Geraldes R., Höckner S., Schiffer E., Claridge T.D., Leppert D., DeLuca G., Kuhle J., Palace J, & Anthony D.C. (2021). Objective biomarkers for clinical relapse in multiple sclerosis: a metabolomics approach. Brain Communications, 3(4), fcab240.
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9

Plasma NfL Quantification Protocol

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Blood samples were collected into purple top (EDTA) tubes and centrifuged at 2000 × g at 4 °C for 8 min within 2 h of collection. Plasma supernatant was collected, divided into aliquots, and frozen at − 80 °C until further use. Samples were thawed on ice before analysis and the concentration of neurofilament light chain (NfL) using a Single Molecule Array Assay kit (NF-light, Quanterix, Lexington, MA), following the manufacturer’s instructions.
Fefer G., Panek W.K., Khan M.Z., Singer M., Westermeyer H., Mowat F.M., Murdoch D.M., Case B., Olby N.J, & Gruen M.E. (2022). Use of Cognitive Testing, Questionnaires and Plasma Biomarkers to Quantify Cognitive Impairment in an Aging Pet Dog Population. Journal of Alzheimer's disease : JAD, 87(3), 1367-1378.
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10

Measuring NfL levels in plasma samples

Cited 2 times
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In the Amsterdam cohort, NfL levels were measured in both EDTA and heparin plasma using the commercially available NfL assay for SIngle MOlecule Array (SIMOA) according the kit description (NF-LIGHT™—Quanterix, Billerica, USA). For EDTA samples the result was converted by factor 1.25 in accordance to pre-analytic studies.11 (link) The MGH cohort samples were analysed using the Olink® Explore 1536 platform, antibody-based multiplex technology with PCR read-out.9 (link) The analytical performance of the proximity extension assay was validated for each protein assay; performance data are available at www.olink.com. Both the SIMOA and the Olink assays use the same antibodies and have been shown to be highly correlated (r = 0.9417 (Olink Target Neuro Exploratory—Olink).
Smeele P.J., Vermunt L., Blok S., Duitman J.W., Nossent E.J., van Agtmael M.A., Heunks L.M., Horn J., Bogaard H.J, & Teunissen C.E. (2022). Neurofilament light increases over time in severe COVID-19 and is associated with delirium. Brain Communications, 4(4), fcac195.
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