Cells were plated in flat bottom 96-well plates (4×103/ well) and treated with BMP-7 for 1‒24 h. After incubation, cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% triton-X 100 in PBS for 10 min, blocked with PBS containing 5% FBS, and incubated with antibodies against FoxC2 (1:200), aggrecan (1:100), SOX9 (1:200), and MMP-2 (1:200) at 4°C overnight. As a negative control, cells were reacted with isotype IgG under similar conditions. After washing, the cells were incubated with DyLightTM488-conjugated goat anti-rabbit antibody and fluorescein- or DyLightTM594-conjugated goat anti-mouse antibody (Jackson Laboratory), at a dilution of 1:200 for 45 min at room temperature. Fluorescence signals were captured using an Olympus Fluoview FV1000 confocal microscope and analyzed by the FV10-ASW 1.6 Viewer program (Olympus, Japan).
Fv10 asw 1.6 viewer program
Manufactured by Olympus
Sourced in Japan
The FV10-ASW 1.6 Viewer program is a software application designed to visualize and analyze images captured using Olympus microscopes. It provides basic image processing and measurement tools for users to view and manipulate acquired data.
Automatically generated - may contain errors
Lab products found in correlation
Fluoview fv1000 confocal microscope, by Olympus (4 mentions)
Anti 53bp1, by Santa Cruz Biotechnology (1 mentions)
Anti htert, by Santa Cruz Biotechnology (1 mentions)
Anti mer11, by Santa Cruz Biotechnology (1 mentions)
Anti trf1, by Santa Cruz Biotechnology (1 mentions)
Anti γ h2ax, by Santa Cruz Biotechnology (1 mentions)
Anti atr, by Santa Cruz Biotechnology (1 mentions)
4 protocols using fv10 asw 1.6 viewer program
1
Immunofluorescence Analysis of Chondrocyte Markers
2
Immunofluorescence Assay for Nucleolin Detection
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Immunofluorescence was performed as previously reported [17 (link)]. Cells were fixed in 2% formaldehyde and permeabilized in 0.25% Triton X100 in phosphate buffered saline (PBS) for 5 min at room temperature. Cells were then incubated with primary antibodies (anti-NCL), washed in PBS and incubated with the fluorophore-conjugated secondary antibodies. The following secondary antibodies were used: Rhodamine or DyLightTM488 conjugated goat anti-rabbit, fluorescein or DyLightTM594 conjugated goat anti-mouse (ZSGB-BIO). Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan).
3
Immunofluorescence Imaging of DNA Damage Markers
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Immunofluorescence was performed as previously reported [51 (link)]. Cells were fixed in 2% formaldehyde and permeabilized in 0.25% Triton X100 in PBS for 5 min at room temperature. For immunolabeling, cells were incubated with primary antibody, washed in PBS and incubated with fluorophore-conjugated secondary antibodies. The following monoclonal primary antibodies were used: anti-TRF1, anti-ATR, anti-γ-H2AX, anti-53BP1, anti-Mer11 and anti-hTERT, (Santa Cruz Biotechnology, Inc.). Rhodamine- or DyLightTM488-conjugated goat anti-rabbit and fluorescein- or DyLightTM594-conjugated goat anti-mouse (ZSGB-BIO) served as secondary antibodies. Fluorescence signals were captured using an Olympus Fluoview FV1000 confocal microscope and analyzed using the FV10-ASW 1.6 Viewer program (Olympus, Japan).
4
Immunofluorescence Analysis of Telomeric Proteins
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Immunofluorescence was performed as previously reported (51 (link)). Cells were fixed in 2% formaldehyde and permeabilized in 0.25% Triton X100 in phosphate buffered saline for 5 min at room temperature. For immunolabeling, cells were incubated with primary antibody and then washed in phosphate buffered saline and incubated with the fluorophore-conjugated secondary antibodies. The following primary antibodies were used: mAb anti-TRF1 (Novus), mAb anti-TRF2 (Novus), pAb anti-POT1 (Sigma), mAb anti-γ-H2AX (Genscript), mAb anti-53BP1 (Novus), mAb anti-hTERT (Rockland) and mAb anti-PCBP1 (Proteintech). The following secondary antibodies were used: Rhodamine or DyLight™488 conjugated goat anti-rabbit, fluorescein or DyLight™594 conjugated goat anti-mouse (Jackson Laboratory). Fluorescence signals were captured by using Olympus Fluoview FV1000 confocal microscope and analyzed by FV10-ASW 1.6 Viewer program (Olympus, Japan).
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