3 amino 1 2 4 triazole atz

Manufactured by Merck Group

Sourced in United States

3-Amino-1,2,4-triazole (ATZ) is a chemical compound used in various laboratory applications. It is a heterocyclic organic compound consisting of a triazole ring with an amino group attached. ATZ serves as a versatile building block and intermediate in the synthesis of other compounds, but its specific core function is not extrapolated upon.

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Triazole (2 citations)

4 protocols using 3 amino 1 2 4 triazole atz

Evaluation of Apoptosis and Stemness

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Hoechst 33342, verapamil, glutaminase, L-asparaginase, and 3-Amino-1,2,4-triazole (ATZ), 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), hydroethidine, Rhodamine 123 were purchased from Sigma (St Louis, MO, USA). Rabbit monoclonal anti-Axin2 (D48G4), rabbit monoclonal anti-Survivin (71G4B7), rabbit polyclonal anti-phospho-β-catenin (Ser33/37/Thr41), rabbit monoclonal anti-phospho-Akt (Ser473) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-c-Myc (9E10) was purchased from Santa Cruz (Santa Cruz, CA, USA). Rabbit polyclonal anti-CyclinD1 antibody was obtained from GeneTex (San Antonio, TX, USA). Mouse monoclonal anti-β-catenin (C47H1), rabbit monoclonal anti-Sox-2, rabbit monoclonal anti-ABCG2, and mouse monoclonal anti-β-actin antibodies were purchased from Abcam (Cambridge, UK). CM-DCFDA, Lipofetamine RNAiMAX and Opti-MEM were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA).

Liao J., Liu P.P., Hou G., Shao J., Yang J., Liu K., Lu W., Wen S., Hu Y, & Huang P. (2017). Regulation of stem-like cancer cells by glutamine through β-catenin pathway mediated by redox signaling. Molecular Cancer, 16, 51.

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Sirt1 and Catalase Inhibition in Senescence

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To investigate if Sirt1 or Cat activity influenced changes in the senescence of the conditioned cells either exposed or not to H₂O₂, their respective activities were inhibited using EX-527 (Sigma, Milan-Italy) at a concentration of 5 μM for 1 h and 3-amino-1,2,4-triazole (ATZ) (Sigma, Milan-Italy) at a concentration of 10 mM for 3 h.

Russomanno G., Corbi G., Manzo V., Ferrara N., Rengo G., Puca A.A., Latte S., Carrizzo A., Calabrese M.C., Andriantsitohaina R., Filippelli W., Vecchione C., Filippelli A, & Conti V. (2017). The anti-ageing molecule sirt1 mediates beneficial effects of cardiac rehabilitation. Immunity & Ageing : I & A, 14, 7.

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Tectorigenin Modulates Cellular Signaling

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Tectorigenin (Fig. 1A) was provided by Professor Dong Hyun Kim (Kyung Hee University, Seoul, Korea). MTT, 3-amino-1,2,4-triazole (ATZ), and BAY 11-7082 ((E)-3-(4-methylphenylsulfonyl)-2-propenenitrile) were purchased from Sigma-Aldrich (St. Louis, MO, USA). An anti-catalase antibody was purchased from Biodesign International Company (Saco, ME, USA). Anti-ERK2 and anti-phospho-ERK1/2 (Thr 202/Tyr 204) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). U0126 was purchased from Calbiochem (San Diego, CA, USA). Anti-β-actin, anti-IκB-α, and anti-NF-κB antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). A primary antibody against TATA-binding protein was purchased from Abcam (Cambridge, MA, USA). The NF-κB-binding site-luciferase construct was a generous gift from Dr. Young Joon Surh (Seoul National University, Seoul, Korea). The other chemicals and reagents were of analytical grade.

Zhang R., Piao M.J., Oh M.C., Park J.E., Shilnikova K., Moon Y.J., Kim D.H., Jung U., Kim I.G, & Hyun J.W. (2016). Protective Effect of an Isoflavone, Tectorigenin, Against Oxidative Stress-induced Cell Death via Catalase Activation. Journal of Cancer Prevention, 21(4), 257-263.

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Comprehensive Protein Profiling for Cancer Research

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Antibodies against IKKβ, catalase, SOD1, p21, p27, p-Chk1(S345), Chk1, p-Chk2(T68), Chk2, p-H2AX(S139), H2AX, p-ATM(S1981), ATM, MGMT, PARP, Caspase-3, p-p53(S15), p53, survivin, XIAP, cIAP-1, cIAP-2, Mre11, Rad50, p95/NBS1, were purchased from Cell Signaling (Beverly, MA). Antibodies against Bak, Bcl-2, Bcl-xL, GAPDH, and actinin were purchased from Santa Cruz Biotechnology, Inc. Fotemustine and antibody against Flag (M2) was purchased from Sigma. 8-OHdG ELISA kit, mirin and SC-514 for in vivo experiment were purchased from Abcam. SC-514 for in vitro experiment, carmustine and lomustine were purchased from Enzo Life Sciences. PLX4032, BMS-345541, TPCA-1, LY-2409881, AZD3264, IKK-16 and PS-1145 were purchased form Selleckchem ML 120B dihydrochloride and PF184 were purchased from Tocris Bioscience. Crystal violet, propidium iodide (PI), N-acetyl-L-cysteine (NAC), temozolomide, 3-Amino-1,2,4-triazole (ATZ), hydrogen peroxide solution and 3-(4,5-Dimethyl-2-thiazolyl)−2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma. 6-Carboxy-2′,7′-dichlorofluorescein diacetate (DCF) was purchased from Thermo Scientific.

Tse A.K., Chen Y.J., Fu X.Q., Su T., Li T., Guo H., Zhu P.L., Kwan H.Y., Cheng B.C., Cao H.H., Lee S.K., Fong W.F, & Yu Z.L. (2017). Sensitization of melanoma cells to alkylating agent-induced DNA damage and cell death via orchestrating oxidative stress and IKKβ inhibition. Redox Biology, 11, 562-576.

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