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5 protocols using sodium tripolyphosphate

1

Fabrication and Evaluation of 5-FU/Chitosan Nanoparticles

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5-Fluorouracil powder was supplied by Aladdin Industrial Corporation (Shanghai, China), while chitosan (deacetylation degree 86%) and sodium tripolyphosphate were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methylthiazolyldiphenyl-tetrazolium bromide conversion (MTT) was purchased from Sigma (Sigma-Aldrich, Germany and USA). Dimethyl sulfoxide (DMSO) was obtained from Sigma (USA). Moreover, the human gastric cancer (SGC-7901) cell line was obtained from Cellbio (Shanghai, China). All other reagents used in the assay were of analytical grade, and the water used in the experiment was doubly distilled and deionized.
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2

Characterization of Potassium Sulfate Fertilizer

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Agricultural potassium sulfate (LN K2SO4) was obtained from SDIC Xinjiang Lop Nur Potash Co. Ltd., China. Chemical grade polyethylene glycol 1000 (PEG 1000), sodium tripolyphosphate (STPP), and analytical grade reagents for gravimetric methods and Volhard method (sodium tetraphenylborate, ethylenediaminetetraacetic acid disodium salt, ammonium iron sulfate, BaCl2 and AgNO3) were purchased from Sinopharm Chemical Reagent Co. Ltd. Industrial grade urea phosphate (UP) was purchased from Tianjin Kemiou Chemical Reagent Co. Ltd. Tap water was used in all experiments and its physicochemical properties are shown in Table 1. The concentration of metal ions (K+, Na+, Mg2+, Ca2+) was determined by an atomic absorption spectrophotometer (AAS, AA-6880 SHIMADZU, Japan). The concentration of SO42− and Cl anions was determined by the barium chloride gravimetric method and the Volhard method, respectively.
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3

Animal Bone Glue and Mineral Components

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The animal bone glue, which was made from animal fur and bone, consisting of yellow solid granules with a pH of 6.8, was from Liaoning Xinxing Co., Ltd, China. The sand ‘ZGS-50/100(60)’, with a moisture content less than 0.3%, was from Dalian, China. Anhydrous sodium carbonate, sodium pyrophosphate, sodium hexametaphosphate and sodium tripolyphosphate were from Sinopharm Chemical Reagent Co., Ltd, China.
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4

Comprehensive Synthesis of HA-HPMC-CS Nanoparticles

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HA (MW: 403.31, content: 97%) was purchased from Macklin (Shanghai, China). Hydroxypropyl methyl cellulose (HPMC, Viscosity: 50–20,000 mPa.s) was obtained from Tianjin guangfu fine chemical research institute. CS (MW:161.16 kDa, viscosity: 50–800 mPa.s, degree of acetylation 80–95%), sodium tripolyphosphate (TPP, MW: 367.86), β-cyclodextrin (β-CD, MW: 1134.98), sodium hydroxide, formic acid and acetic acid were purchased from Sinopharm group chemical reagent co. Ltd. Ethyl alcohol (95%) was bought from Wuhan xingheda trading co. Ltd. Poloxamer 188 were purchased from sigma-aldrich, USA. Methanol (HPLC) was purchased from Grace chemical technology co., LTD. Acetonitrile was bought from Sigma-Alorich. Sodium chloride (NaCl, ≥99.5%) were purchased from Wuxi jiani chemical co. Ltd. Ultrapure water was prepared by a Microporous Purification® Purified Water System (Milli-Q Ltd., France). All other reagents not special emphasis were of analytical pure.
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5

Chitosan-based Bioactive Compound Synthesis

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Chitosan with a degree of deacetylation of 90% (Mw = 50 k Da,) was purchased from Golden-Shell Pharmaceutical Co., Ltd. (Zhejiang, China). The 2-Benzimidazolecarboxylic acid (BIM), isopropyl alcohol, sodium hydroxide, 2,3-epoxypropyltrimethyl ammonium chloride (EPTAC), sodium tripolyphosphate (TPP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, tris(hydroxymethyl)methyl aminomethane (Tris), reduced coenzyme I (NADH I), hydrochloric acid, phenazine methosulfate (PMS), and nitroblue tetrazolium (NBT) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The 2% rabbit erythrocyte suspension (Sbjbio, Nanjing, China). The mouse fibroblast cell line (L929), human lung adenocarcinoma epithelial cell line (A549), and human breast cancer cell line (MCF-7) were provided by the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. All the reagents were analytical grade and used without further purification.
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