Unity inova 500 ft nmr

Measurements of optical rotations, IR, and circular dichroisms spectra were performed on the JASCO P-1020 polarimeter, FT/IR-4100 infrared spectrophotometer (JASCO Corporation, Tokyo, Japan), and Jasco J-715 CD spectrometer, respectively. LRESIMS were measured on a Bruker APEX II (Bruker, Bremen, Germany) mass spectrometer, and HRESIMS were measured on the Bruker APEX II and the Impact HD Q-TOF mass spectrometers (both Bruker, Bremen, Germany). The NMR experiments were recorded on a Varian Unity INOVA 500 FT-NMR (Varian Inc., Palo Alto, CA, USA). The silica gel (40−63 μm, Merck, Billerica, MA, USA) and reversed-phase silica gel (RP-18, 40−63 μm, Merck, Darmstadt, Germany) were used for column chromatography. The thin-layer chromatography (TLC) analysis was carried out on aluminum plates coated with silica gel (Silica gel 60 F254, 100 μm, Merck, Darmstadt, Germany) or C18 gel (Silica gel 60 RP-18 F₂₅₄s, 100 μm, Merck, Darmstadt, Germany). High-performance liquid chromatography (HPLC) was performed on a Hitachi L-2455 HPLC apparatus (Hitachi, Tokyo, Japan) with a Supelco C18 column (250 × 21.2 mm, 5 μm, Supelco, Bellefonte, PA, USA).

Measurements of circular dichroisms, optical rotations, and IR spectra were carried out on a Jasco J-715 CD spectrometer, JASCO P-1020 polarimeter, and FT/IR-4100 infrared spectrophotometer (JASCO Corporation, Tokyo, Japan), respectively. ESIMS was performed on a Bruker APEX II (Bruker, Bremen, Germany) mass spectrometer, and HRESIMS was performed on a Bruker APEX II and Impact HD Q-TOF mass spectrometers (Bruker, Bremen, Germany). The NMR spectra were recorded on a Varian 400MR FT-NMR at 400 and 100 MHz for ¹H and ¹³C, respectively; a Varian Unity INOVA500 FT-NMR (both Varian Inc., Palo Alto, CA, USA) at 500 and 125 MHz for ¹H and ¹³C, respectively; or a JEOL ECZ600R FT-NMR (Japan) at 600 and 150 MHz for ¹H and ¹³C, respectively. Silica gel and reversed-phase (RP-18, 230–400 mesh) silica gel were used for column chromatography and analytical thin-layer chromatography (TLC) analysis (Kieselgel 60 F-254, 0.2 mm, Merck, Darmstadt, Germany), respectively. The isolation and purification of compounds by high-performance liquid chromatography (HPLC) were achieved using a Hitachi L-2455 HPLC apparatus (Hitachi, Tokyo, Japan) equipped with a Supelco C18 column (250 × 21.2 mm, 5 μm, Supelco, Bellefonte, PA, USA).

Values of the specific optical rotation of the isolates were measured with a JASCO P-1020 polarimeter (JASCO Corporation, Tokyo, Japan). Infrared spectra were recorded using a JASCO FT/IR-4100 infrared spectrophotometer (JASCO Corporation, Tokyo, Japan). The CD spectrum was recorded on a Jasco J-815 circular dichroism (CD) spectropolarimeter (JASCO, Tokyo, Japan) in MeOH. ¹H- and ¹³C-NMR spectra were acquired on a Varian 400MR FT-NMR (or Varian Unity INOVA500 FT-NMR) instrument (Varian Inc., Palo Alto, CA, USA) at 400 MHz (or 500 MHz) and 100 MHz (or 125 MHz), respectively, in CDCl₃ or C₆D₆. LR-ESI-MS and HR-ESI-MS experiments were carried out using a Bruker APEX II (Bruker, Bremen, Germany) mass spectrometer. Silica gel (230–400 mesh) was used as the adsorbent for normal-phase column chromatography. Thin-layer chromatography (TLC) analyses were performed with precoated silica gel plates (Kieselgel 60 F-254, 0.2 mm, Merck, Darmstadt, Germany). Further purification of impure fractions or compounds was further achieved by high-performance liquid chromatography on a Hitachi L-7100 HPLC instrument (Hitachi Ltd., Tokyo, Japan) with a Merck Hibar Si-60 column (250 mm × 21 mm, 7 μm; Merck, Darmstadt, Germany) and on a Hitachi L-2455 HPLC apparatus (Hitachi, Tokyo, Japan) with a Supelco C18 column (250 mm × 21.2 mm, 5 μm; Supelco, Bellefonte, PA, USA).

Optical rotations were measured on a JASCO P-1020 polarimeter (Jasco Corporation, Tokyo, Japan). IR spectra were recorded on a JASCO FT/IR-4100 spectrophotometer (Jasco). ESIMS and HRESIMS data were performed on a BRUKER APEX II mass (Bruker, Bremen, Germany) spectrometers. The NMR spectra were recorded on a Varian Unity INOVA 500 FT-NMR (Varian Inc., Palo Alto, CA, USA) at 500 MHz for ¹H and 125 MHz for ¹³C or on a Varian 400 FT-NMR (Varian Inc.) at 400 MHz for ¹H and 100 MHz for ¹³C in CDCl₃ or C₆D₆ using TMS as internal standard (δ in ppm, J in Hz). Silica gel 60 (230–400 mesh, Merck, Darmstadt, Germany) pre-coated silica gel plates (Merck, Kieselgel 60 F254, 0.2 mm) were used for open CC and analytical TLC analysis, respectively. Isolation by HPLC was performed by a Hitachi L-2455 instrument (Hitachi Ltd., Tokyo, Japan) equipped with a reversed-phase (RP-18) column (ODS-3, 5 µm, 250 × 20 mm, Sciences Inc., Tokyo, Japan).

Optical rotations and IR spectra were measured on a JASCO P-1020 polarimeter and a JASCO FT/IR-4100 (JASCO Corporation, Tokyo, Japan) spectrophotometer, respectively. HRESIMS spectra were measured on a Bruker APEX II mass spectrometer (Bruker, Bremen, Germany). ¹H and ¹³C NMR spectra were obtained on a Varian Unity INOVA 600 FT-NMR (or Varian Unity INOVA500 FT-NMR) instruments (Varian Inc., Palo Alto, CA, USA) at 600 MHz (or 500 MHz) for ¹H, and 150 MHz (or 125 MHz) for ¹³C in CDCl₃. Thin-layer chromatography (TLC) analyses were performed on precoated silica (Si) gel plates (Kieselgel 60 F-254, 0.2 mm), and Si gel (230–400 mesh) (Merck, Darmstadt, Germany) and C18-reversed phase Si gel (RP-18; 40–63 µM) (Parc-Technologique BLVD, Quebec, Canada) were used for column chromatography. Further purification and the isolation of compounds were performed by reversed-phase high-performance liquid chromatography (RP-HPLC) on a Hitachi L-2455 HPLC apparatus with a Supelco C18 column (250 × 21.2 mm, 5 μm).