Chemoffice v20

Compounds that reached inhibitions of 50% and that were active at more than one concentration were statistically analyzed to determine their IC₅₀ using GraphPad Prism v.5.00 software package (GraphPad Software, Inc., San Diego, CA, USA). The bioactivity data were fitted to a sigmoidal dose–response model with variable slope. Calculation of CLogp was performed using ChemOffice v20.1 (PerkinElmer, Wal-tham, MA, USA) using the appropriate tool in ChemDraw Professional [36 (link)]. All bioassays were performed using a completely randomized design. Percentage data were approximated to normal frequency distribution by means of angular transformation. Then, percentage data were subjected to analysis of variance (ANOVA). The significance of mean differences among treatments was evaluated by Tukey test at p < 0.05. Statistical analysis was performed using SPSS software 27 (SPSS Inc., Chicago, IL, USA).

Growth inhibition assays on broomrape species were performed using a completely randomized design. Percentage data were transformed using angular transformation to normalize data and stabilize variances. Analysis of variance (ANOVA) was conducted using SPSS software 27 (SPSS Inc., Chicago, IL, USA). The significance of mean differences among treatments was evaluated by Tukey’s multiple comparison test (p < 0.05).
Calculation of clog P values was performed using ChemOffice v20.1 (PerkinElmer, Waltham, MA, USA) using the appropriate tool in ChemDraw Professional, while the number of rotable bonds, H-bond acceptors, and H-bond donors was calculated using the SwissADME software [48 (link),49 (link),50 ].