Luc hek293 cell line

Manufactured by BPS Biosciences

The Luc)-HEK293 cell line is a genetically engineered cell line derived from human embryonic kidney cells (HEK293) that stably express the luciferase (Luc) reporter gene. This cell line can be used for a variety of applications, including reporter gene assays and drug discovery screening. The core function of this cell line is to serve as a tool for researchers to measure luciferase activity as a readout for gene expression or cellular activity.

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Lab products found in correlation

Diethyl phosphite, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Methyl 3 5 dimethylbenzoiate, by Merck Group (3 mentions) Anti gst tb, by PerkinElmer (3 mentions) Streptavidin d2, by PerkinElmer (3 mentions) N bromosuccinimide, by Merck Group (3 mentions) Cell culture media, by Thermo Fisher Scientific (3 mentions) One step luciferase assay system, by BPS Biosciences (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) 2 2 dipyridyl disulfide, by Merck Group (3 mentions) Dual luciferase reporter assay kit, by Promega (1 mentions) Fluostar optima microplate reader, by BMG Labtech (1 mentions)

4 protocols using luc hek293 cell line

Reagents and Materials for Peptide Synthesis

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Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, Ill.), NovaBiochem (La Jolla, Calif.), or Anaspec (San Jose, Calif.). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, Calif.). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, Mo.). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, Mass.). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, Calif.).

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US11266713B2. Di-sulfide containing cell penetrating peptides and methods of making and using thereof (2022-03-08). Ohio State Innovation Foundation [US]. Inventors: Dehua Pei [US], Ziqing Qian [US].

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Reagents and Materials for Peptide Synthesis

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Example 1

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US11351222B2. Di-sulfide containing cell penetrating peptides and methods of making and using thereof (2022-06-07). Ohio State Innovation Foundation [US]. Inventors: Dehua Pei [US], Ziqing Qian [US].

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NF-κB Reporter Assay with 4E-BP2

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The NF-κB reporter (luc)-HEK293 cell line (BPS Bioscience) contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. Stable cells were transfected with 4E-BP2 isoforms as above and supplemented with 100 ng pTK-RL (Gkogkas et al., 2008 (link)) for 48h and lysed 24h later (+/− TNFα 1nm) in lysis buffer from the Dual Luciferase Reporter Assay kit (Promega). Firefly and renilla luminescence were measured using a FLUOstar OPTIMA micro-plate reader (BMG LABTECH). Firefly luciferase luminescence values were normalized to renilla firefly luminescence values and are averages of four experiments.

Kouloulia S., Hallin E.I., Simbriger K., Amorim I.S., Lach G., Amvrosiadis T., Chalkiadaki K., Kampaite A., Truong V.T., Hooshmandi M., Jafarnejad S.M., Skehel P., Kursula P., Khoutorsky A, & Gkogkas C.G. (2019). Raptor-Mediated Proteasomal Degradation of Deamidated 4E-BP2 Regulates Postnatal Neuronal Translation and NF-κB Activity. Cell Reports, 29(11), 3620-3635.e7.

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Peptide Synthesis Reagents and Cell Culture Protocols

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Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, IL), NovaBiochem (La Jolla, CA), or Anaspec (San Jose, CA). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, CA). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, MO). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, MA). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, CA).

[Figure (not displayed)]

US11878046B2. Di-sulfide containing cell penetrating peptides and methods of making and using thereof (2024-01-23). Ohio State Innovation Foundation [US]. Inventors: Dehua Pei [US], Ziqing Qian [US].

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