Monocytes were first incubated with 200 μg/ml of purified human IgG (Sigma) in PBS supplemented with 2 mM EDTA and 1% FCS for 5 min at 4°C to prevent unspecific binding through Fc receptors. Incubations with specific antibodies were carried out for 15 min at 4°C with ready-to-use concentrations of FITC-conjugated antibodies against CD14 (BD Pharmingen, San Jose, CA, USA) and PE-conjugated antibodies against CD54 (BD Pharmingen) or APC-conjugated antibody against CD11a (BD Pharmingen). Labeled cells were washed with PBS and re-suspended in PBS supplemented with 2 mM EDTA and 0.5% BSA. The data was acquired on a Dako Cyan flow cytometer (Beckman Coulter, Fullerton, CA, USA) and analyzed with the Summit software.
Fitc conjugated antibodies against cd14
Manufactured by BD
Sourced in United States
FITC-conjugated antibodies against CD14 are laboratory reagents used in flow cytometry and other immunoassays. They specifically bind to the CD14 antigen expressed on the surface of certain immune cells, such as monocytes and macrophages. The FITC fluorescent label allows for the detection and identification of CD14-positive cells.
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Lab products found in correlation
2 protocols using fitc conjugated antibodies against cd14
1
Monocyte Immunophenotyping by Flow Cytometry
2
Immunophenotyping of Dental Stem Cells
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The SHEDs were collected and digested into single cells using trypsin. According to the cell count result, 106 cells were added to each centrifuge tube. Antibodies including FITC-conjugated antibodies against CD14, CD19, CD45, CD90, and CD105 (BD Biosciences, CA, USA) and PE-conjugated antibodies against CD73 (BD Biosciences, CA, USA), were added into the centrifuge tube respectively. In this experiment, the antibodies FITC Mouse IgG1, κ Isotype Control, and PE Mouse IgG1, κ Isotype Control (BD Biosciences, CA, USA) were used as the isotype control groups for FITC-conjugated and PE-conjugated antibodies. SHEDs were incubated with antibodies in the dark for 30 min, and the results were detected using flow cytometry (BD Biosciences, CA, USA). The Flow Jo V10 software was used to analyze the expression levels of positive cells in the relevant population. The experiment was repeated at least three times using biological samples and technical means.
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