Mouse ana total ig elisa kit

Manufactured by Alpha Diagnostic

Sourced in United States

The Mouse ANA Total Ig ELISA Kit is a laboratory product used for the detection and quantification of total immunoglobulin (Ig) antibodies in mouse samples. It employs an enzyme-linked immunosorbent assay (ELISA) technique to measure the levels of these antibodies.

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Lab products found in correlation

Nunc maxisorp plate, by Thermo Fisher Scientific (1 mentions) Mouse igg total elisa kit, by Thermo Fisher Scientific (1 mentions) Hemavet 950, by Drew Scientific (1 mentions) Mouse anti dsdna total ig elisa kit, by Alpha Diagnostic (1 mentions)

3 protocols using mouse ana total ig elisa kit

Quantifying Autoantibodies and Complement

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Plasma levels of Antinuclear Antibodies (ANA), anti-dsDNA antibodies, and complement 3 (C3) were measured using a Mouse ANA Total Ig ELISA Kit (Catalog No. 5210, Alpha Diagnostic, San Antonio, Texas, USA), a Mouse anti-dsDNA Antibodies Total Ig ELISA Kit (Catalog No. 5110, Alpha Diagnostic, San Antonio, Texas, USA), and a Mouse C3 ELISA Kit (Catalog No. 6270, Alpha Diagnostic, San Antonio, Texas, USA), respectively, according to the manufacturer’s instructions.

Guo F., Pan Q., Chen T., Liao S., Li S., Li A., Chen S., Chen J., Xiao Z., Su H., Yang L., Yang C., Liu H.F, & Pan Q. (2023). hUC-MSC transplantation therapy effects on lupus-prone MRL/lpr mice at early disease stages. Stem Cell Research & Therapy, 14, 211.

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Quantifying Mouse Serum IgG and ANA

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Mouse serum or plasma was assessed via the IgG (Total) Mouse ELISA Kit (Thermo Fisher), according to the manufacturer’s directions, using 96-well Nunc Maxisorp plates (Thermo Fisher) with samples diluted 1:20,000 – 1:40,000. Serum or plasma was assessed for ANA using the Mouse ANA Total Ig ELISA Kit (Alpha Diagnostic International), according to the manufacturer’s directions, with samples diluted 1:50. Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.

Reighard S.D., Krishnamurthy D., Cevik H., Ochayon D.E., Ali A., Seelamneni H., Brunner H.I, & Waggoner S.N. (2020). Immunomodulatory effects of cytokine-induced expansion of cytotoxic lymphocytes in a mouse model of lupus-like disease. Cytotherapy, 23(1), 37-45.

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Comprehensive Murine Immune Assessment

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Blood samples were collected by either retro-orbital eye bleeds under isoflurane anesthesia or cardiac puncture after euthanasia by CO₂ inhalation in accordance with Regeneron IACUC protocol. CBC with differential was performed on 20 μL of whole blood using Hemavet 950 (Drew Scientific Group) and clinical chemistry was completed on serum samples using ADVIA 1800 Chemistry System (Siemans Medical Solutions USA). ELISAs were performed on plasma samples using the following; Mouse IgG and IgM Rheumatoid Factor ELISA Kit (Shibayagi Co., Ltd), Mouse Anti-dsDNA Total Ig ELISA kit, Mouse ANA Total Ig ELISA kit, Mouse Anti-Sm Total Ig ELISA kit, Mouse Anti-Cardiolipin Total Ig ELISA kit (Alpha Diagnostic Intl.), and IgG and IgM mouse ELISA kit (Abcam) as per the manufacturer’s instructions. IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12total, IL-17, MCP-1, and TNF-α were measured in plasma samples using a Multi-Spot® 10plex electrochemiluminescence detection assay (Meso Scale Discovery) according to the manufacturer’s instructions.

Atanasio A., Decman V., White D., Ramos M., Ikiz B., Lee H.C., Siao C.J., Brydges S., LaRosa E., Bai Y., Fury W., Burfeind P., Zamfirova R., Warshaw G., Orengo J., Oyejide A., Fralish M., Auerbach W., Poueymirou W., Freudenberg J., Gong G., Zambrowicz B., Valenzuela D., Yancopoulos G., Murphy A., Thurston G, & Lai K.M. (2016). C9orf72 ablation causes immune dysregulation characterized by leukocyte expansion, autoantibody production, and glomerulonephropathy in mice. Scientific Reports, 6, 23204.

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