Fitc or texas red secondary antibodies

Manufactured by Abcam

FITC- or Texas-Red secondary antibodies are fluorescently labeled antibodies used in immunoassays, flow cytometry, and microscopy applications. They bind to primary antibodies and emit fluorescent signals upon excitation, allowing for the detection and visualization of target proteins or cells.

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Lab products found in correlation

Saponin, by Merck Group (5 mentions) Protein block solution, by Agilent Technologies (4 mentions) Tunel solution, by Roche (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Ab15580, by Abcam (3 mentions) A7811, by Merck Group (3 mentions) Ab16669, by Abcam (3 mentions) Mca48r, by Bio-Rad (3 mentions) Epi fluorescence microscopy system, by Olympus (2 mentions) A5228, by Merck Group (2 mentions) Ab10559, by Abcam (2 mentions) Ab6994, by Abcam (2 mentions) Confocal microscopy system, by Zeiss (1 mentions) Anti von willebrand factor, by Abcam (1 mentions) Ht15 trichrome staining masson kit, by Merck Group (1 mentions) Epifluorescence microscope, by Olympus (1 mentions) Ab955, by Abcam (1 mentions) Lsm 880, by Zeiss (1 mentions) Prolong gold mounting media, by Thermo Fisher Scientific (1 mentions) Ab2254, by Abcam (1 mentions)

Spelling variants of this product

Texas Red (6 citations)

5 protocols using fitc or texas red secondary antibodies

Immunohistochemical Analysis of Cardiac Tissue

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For immunohistochemistry staining, heart cryosections were fixed with 4% paraformaldehyde, permeabilized and blocked with Protein Block Solution (DAKO, Carpinteria, CA) containing 0.1% saponin (Sigma, St Louis, MO), and then incubated with the following antibodies overnight at 4 °C: mouse anti-alpha sarcomeric actin (1:100, a7811, Sigma), rabbit anti-CD45 (1:100, ab10559, Abcam, Cambridge, United Kingdom), mouse anti-Actin, α-Smooth Muscle antibody (1:100, A5228, Sigma), rabbit anti-Ki67 (1:100, ab15580, Abcam), rabbit anti-CD3 (1:100, ab16669, Abcam) and mouse anti-CD8 alpha (1:100, mca48r, abd Serotec, Raleigh, NC ). FITC- or Texas-Red secondary antibodies (1:100) were obtained from Abcam Company and used for the conjunction with these primary antibodies. For assessment of cell apoptosis, heart cryosections were incubated with TUNEL solution (Roche Diagnostics GmbH, Mannheim, Germany) and counter-stained with DAPI (Life Technology, NY, USA). For assessment of angiogram, heart cryosections were incubated with Lectin (FL-1171, Vector laboratories, Burlingame, CA, USA). Images were taken by an Olympus epi-fluorescence microscopy system.

Tang J., Shen D., Caranasos T.G., Wang Z., Vandergriff A.C., Allen T.A., Hensley M.T., Dinh P.U., Cores J., Li T.S., Zhang J., Kan Q, & Cheng K. (2017). Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome. Nature Communications, 8, 13724.

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Cardiac Fibrosis Assessment in Mice

Cited 1 time

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All animals were killed 8 days after treatment. Mouse hearts were harvested and frozen in Optimal Cutting Temperature (OCT) compound (Tissue-Tek, Torrance, CA, USA). Cryo-sections (5 μm thick) were prepared. Masson’s trichrome staining was performed as per manufacturer’s instructions [HT15 Trichrome Staining (Masson) Kit; Sigma-Aldrich]. Fibrosis area was measured by NIH Image J as previously described 23 (link). For immunofluorescence staining, mouse heart cryosections were fixed with 4% PFA, blocked/permeabilized with Protein Block Solution (DAKO) containing 1% saponin (Sigma-Aldrich), and then stained with anti-α-SA (Sigma-Aldrich) and anti-von Willebrand factor (Abcam) antibodies. FITC or Texas-Red secondary antibodies were obtained from Abcam and used in conjunction with these primary antibodies. Images were taken by a Zeiss (Jena, Germany) confocal microscopy system.

Hensley M.T., de Andrade J., Keene B., Meurs K., Tang J., Wang Z., Caranasos T.G., Piedrahita J., Li T.S, & Cheng K. (2015). Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts. Journal of Cellular and Molecular Medicine, 19(8), 1805-1813.

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Immunohistochemical Analysis of Heart Tissue

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Example 11

Histology:

For immunohistochemistry staining, heart cryosections were fixed with 4% paraformaldehyde, permeabilized and blocked with Protein Block Solution (DAKO, Carpinteria, Calif.) containing 0.1% saponin (Sigma, St. Louis, Mo.), and then incubated with the following antibodies overnight at 4° C.: mouse anti-alpha sarcomeric actin (1:100, a7811, Sigma), rabbit anti-CD45 (1:100, ab10559, Abcam, Cambridge, United Kingdom), mouse anti-Actin, α-Smooth Muscle antibody (1:100, A5228, Sigma), rabbit anti-Ki67 (1:100, ab15580, Abcam), rabbit anti-CD3 (1:100, ab16669, Abcam) and mouse anti-CD8 alpha (1:100, mca48r, abd Serotec, Raleigh, N.C.). FITC- or Texas-Red secondary antibodies (1:100) were obtained from Abcam Company and used for the conjunction with these primary antibodies. For assessment of cell apoptosis, heart cryosections were incubated with TUNEL solution (Roche Diagnostics GmbH, Mannheim, Germany) and counter-stained with DAPI (Life Technology, NY, USA). For assessment of angiogram, heart cryosections were incubated with Lectin (FL-1171, Vector laboratories, Burlingame, Calif., USA). Images were taken by an Olympus epi-fluorescence microscopy system.

US11564889B2. Stem cell biomimetic microparticles (2023-01-31). North Carolina State University [US]. Inventors: Ke Cheng [US], Junnan Tang [US].

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Immunohistochemical Staining of Frozen Sections

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For immunohistochemistry staining, frozen cryosections were fixed with 4% paraformaldehyde, permeabilized, and blocked with protein block solution (DAKO, Carpinteria, CA) containing 0.1% saponin (Sigma-Aldrich), and then incubated with antibodies overnight at 4 °C. The following are the primary antibodies used in this study: rabbit anti-CD3 (ab16669, Abcam, Cambridge, U.K.), mouse anti-CD8 α (mca48r, abd Serotec, Raleigh, NC), mouse anti-CD68 (ab955, Abcam) and rabbit anti-vWF (ab6994, Abcam). FITC- or Texas Red secondary antibodies (Abcam) were used in conjunction with the above primary antibodies. TUNEL solution (Roche Diagnostics GmbH, Mannheim, Germany) was applied to cryosections to evaluate their level of cell apoptosis. Images were taken with an Olympus epifluorescence microscope.

Cui X., Tang J., Hartanto Y., Zhang J., Bi J., Dai S., Qiao S.Z., Cheng K, & Zhang H. (2018). NIPAM-based Microgel Microenvironment Regulates the Therapeutic Function of Cardiac Stromal Cells. ACS applied materials & interfaces, 10(44), 37783-37796.

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Cardiac Tissue Characterization Protocol

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Heart cryosections were fixed with 4% paraformaldehyde solution, permeabilized and blocked with 0.01% saponin (Sigma-Aldrich) Dako solution, and then incubated with primary antibodies overnight at 4°C: mouse anti–α-SA (1:100; a7811, Sigma), rabbit anti-Ki67 (1:100; ab15580, Abcam), rabbit anti–von Willebrand factor (1:100; ab6994, Abcam), rabbit anti- pH3 (1:100; ab5176, Abcam), rabbit anti–Aurora B (1:100; ab2254, Abcam). FITC- or Texas Red secondary antibodies (1:200) were obtained from Abcam Company and used with these primary antibodies for 1.5 hour at room temperature. Samples were then treated with DAPI (Life Technologies) and mounted in Prolong Gold Mounting Media (Life Technologies). For assessment of cell apoptosis, heart cryo-sections were incubated with TUNEL solution (Roche Diagnostics GmbH) and counter-stained with DAPI (Life Technologies). Images were taken with an epifluorescent microscope (Olympus IX81) and confocal fluorescent microscope (ZEISS LSM 880). Tissue morphology was characterized from the images using the NIH ImageJ software and ZEN lite software.

Huang K., Ozpinar E.W., Su T., Tang J., Shen D., Qiao L., Hu S., Li Z., Liang H., Mathews K., Scharf V., Freytes D.O, & Cheng K. (2020). An off-the-shelf artificial cardiac patch improves cardiac repair after myocardial infarction in rats and pigs. Science translational medicine, 12(538), eaat9683.

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