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Illustra rnaspin isolation kit

Manufactured by GE Healthcare
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Sourced in United Kingdom

The Illustra RNAspin Isolation Kit is a laboratory equipment product designed for the purification of total RNA from a variety of biological samples. It utilizes a silica-based membrane technology to efficiently capture and purify RNA, enabling its subsequent use in various downstream applications.

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Lab products found in correlation

Agilent 6000 rna nano chips, by Agilent Technologies (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Illustra RNAspin Mini RNA Isolation Kit Illustra RNAspin Mini Isolation Kit Illustra RNAspin 96 RNA isolation kit Illustra RNAspin Midi RNA isolation kit Illustra RNAspin RNA Isolation Kit Illustra RNAspin kit

2 protocols using illustra rnaspin isolation kit

1

Total RNA Extraction and Quality Control

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Total RNA was extracted using the Illustra RNAspin Isolation Kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK) following manufacturer’s instructions. The quality and concentration of the RNA were checked by measuring the OD 260/280 and OD 260/230. RNA degradation was analyzed by microfluidic electrophoresis using Agilent 6000 RNA Nano chips in an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Samples with an RNA integrity number (RIN) ≥ 9.0 were used.
Bitencourt T.A., Macedo C., Franco M.E., Rocha M.C., Moreli I.S., Cantelli B.A., Sanches P.R., Beleboni R.O., Malavazi I., Passos G.A., Marins M, & Fachin A.L. (2019). Trans-chalcone activity against Trichophyton rubrum relies on an interplay between signaling pathways related to cell wall integrity and fatty acid metabolism. BMC Genomics, 20, 411.
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2

Quantitative PCR of DENV RNA

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Huh7 cells were infected and treated with etomoxir or AICAR as described for the flow cytometry experiments. Total cellular RNA was extracted with a GE Healthcare illustra RNAspin isolation kit. Reverse transcription was performed using a High-Capacity cDNA reverse transcription kit (Applied Biosystems) according to the instructions of the manufacturer. Quantitative PCR of DENV RNA was performed as described previously (46 (link)) using TaqMan Universal PCR master mix (Applied Biosystems). Data are represented with relative quantification values determined using the threshold cycle (2−ΔCT) formula where ΔCT corresponds to CTtreated − CTcontrol.
Fernandes-Siqueira L.O., Zeidler J.D., Sousa B.G., Ferreira T, & Da Poian A.T. (2018). Anaplerotic Role of Glucose in the Oxidation of Endogenous Fatty Acids during Dengue Virus Infection. mSphere, 3(1), e00458-17.
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