The microfilters are produced using a 10 μm thick modified SU-8 (MicroChem Corp., Newton, MA). The photo definable negative resist is sensitive to UV radiation.35 ,36 (link) Briefly, the resist is coated on a substrate, an optical mask is used to pattern the pores with an MA6 mask aligner (SÜSS MicroTec AG, Garching, Germany).36 (link) After the pores are opened in a developer, the resist is released from the substrate.36 (link) Each optical mask is patterned to produce thirty 13 μm diameter microfilters within a 100 mm wafer (Fig. 1A ). The pores are formed within a 9 mm diameter area in the center of the filter. Close up scanning electron microscope (SEM) images of the pores are shown in Fig. 1B and C . Pore sizes were measured for each wafer with an Axioplot microscope (Carl Zeiss) using Scopephoto (Scopetek) measurement software which was pre-calibrated with a calibration slide (Oplenic). Three different optical masks with different pore sizes were used to produce different pore distributions: ~70 000, ~110 000 or ~160 000 pores.
Axioplot microscope
Manufactured by Zeiss
Sourced in Germany
The Axioplot microscope is a laboratory equipment designed for optical microscopy. It provides high-quality imaging and analysis capabilities for a variety of scientific applications. The core function of the Axioplot is to enable detailed observation and examination of specimens under magnification.
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Lab products found in correlation
2 protocols using axioplot microscope
1
Fabrication of Microfilters with Tunable Pore Sizes
2
Andrographolide Regulates Astrocyte HO-1 Expression
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Astrocytes plated on glass coverslips were treated with andrographolide, then fixed with 4 % paraformaldehyde/PBS for 15 min, and washed thrice with PBS followed by incubation with permeabilizing buffer containing 0.1 % Triton-X100 in PBS for 5 min at 25 °C. The cells were then incubated in blocking solution (5 % BSA in permeabilizing buffer) for 1 h before incubation overnight with primary antibodies against HO-1 (1:200 dilution) in blocking solution at 4 °C. Subsequently, cells were washed thrice with PBS and incubated with anti-mouse IgG Alexa Flour® 488 (1:400 dilution, Cell Signaling Technology, Danvers, MA, USA) for 1 h at 25 °C. Cells were then washed with PBS before mounting the coverslips onto glass slides using mounting medium containing DAPI nuclear stain (Vector Laboratories, Burlingame, CA, USA). Immunofluorescence images were taken with an Axioplot microscope equipped with Carl Zeiss 510 confocal imaging scan-head and software (Carl Zeiss MicroImaging, Jena, Germany), with the same parameters used for all images.
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