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Psuper retro rnai plasmid

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The PSuper.Retro-RNAi plasmid is a laboratory tool used for RNA interference (RNAi) studies. It is designed to express short hairpin RNA (shRNA) sequences, which can be used to silence the expression of target genes in cell lines and model organisms.

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Lab products found in correlation

Genemute sirna transfection reagent, by SignaGen (1 mentions)

Spelling variants of this product

PSuper-Retro (5 citations) PSUPER.retro plasmid (4 citations)

12 protocols using psuper retro rnai plasmid

1

Plasmid-based RNAi targeting VCP, GP78, and HRD1

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Double-stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro-RNAi plasmid (Oligoengine). The following sequences were targeted for VCP mRNA: 5′- GTAATCTCTTCGAGGTATA-3′; GP78 mRNA: 5′- ACGCTCAGTTGAAATAACA -3′; HRD1 mRNA: 5′- CCGCCATGCTGCAGATCAA -3′. iRhom2- and RNF5-shRNA plasmids were previously described[15 (link),21 (link)].
Luo W.W., Li S., Li C., Zheng Z.Q., Cao P., Tong Z., Lian H., Wang S.Y., Shu H.B, & Wang Y.Y. (2017). iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA. PLoS Pathogens, 13(11), e1006693.
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2

Cloning oligonucleotides into pSuper.Retro-RNAi

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Double‐stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro‐RNAi plasmid (Oligoengine).
Jin J., Zhao L, & Li Z. (2016). The E3 ubiquitin ligase RNF135 regulates the tumorigenesis activity of tongue cancer SCC25 cells. Cancer Medicine, 5(11), 3140-3146.
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3

Silencing Human USP44 Gene Expression

Cited 1 time
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Double-stranded oligonucleotides corresponding to the targeting sequences were cloned into the pSuper-Retro-RNAi plasmid (Oligoengine) as previously described [70 (link)–72 (link)]. The following sequences were targeted for Human USP44-RNAi: #1 5’- GTAACAGGATTGAGAAATT-3’, #2 5’- GGAATTTCTTTGTGAACTT-3’; Human USP44-siRNA: 5’- GAATTGGAGTATCAAGTTA-3’. The siRNA was delivered into HFF cells by GeneMute siRNA Transfection Reagent (SignaGen Laboratories) according to the protocol provided by the manufacturer.
Zhang H.Y., Liao B.W., Xu Z.S., Ran Y., Wang D.P., Yang Y., Luo W.W, & Wang Y.Y. (2020). USP44 positively regulates innate immune response to DNA viruses through deubiquitinating MITA. PLoS Pathogens, 16(1), e1008178.
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4

Mex3 RNA Interference Knockdown

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Double-strand oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro-RNAi plasmid (Oligoengine). The targeted sequences for human Mex3B mRNA were as follows: #1-GCAAGAAGAGCGTGAACAT; #2-GAGAGGAGATTGAGGCGCA; #3-CAGCAGACGCACACGTACA; #4-CGGCAAGGTTGTAAAATCA. The targeted sequences for the other three human Mex3s were as follows: GACCAACACCTACATCAAG (Mex3A); GGAAGTGGCTCTACAGATT (Mex3C); GCCATCCGAATATTCTCCT (Mex3D).
Yang Y., Wang S.Y., Huang Z.F., Zou H.M., Yan B.R., Luo W.W, & Wang Y.Y. (2016). The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response. Cell Research, 26(3), 288-303.
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5

Cloning Target Sequences with pSuper.Retro-RNAi Plasmid

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Double-stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro-RNAi plasmid (Oligoengine). The following sequence was targeted for MDA5 mRNA: 5′-GCTTCAGGAATCTCATCTTAT-3′. TRIM38-, SENP1-, and SENP2-shRNA plasmids were previously described (Ran et al., 2011 (link); Hu et al., 2014 (link)).
Hu M.M., Liao C.Y., Yang Q., Xie X.Q, & Shu H.B. (2017). Innate immunity to RNA virus is regulated by temporal and reversible sumoylation of RIG-I and MDA5. The Journal of Experimental Medicine, 214(4), 973-989.
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6

Plasmid-based RNAi targeting PKACs

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Double-strand oligonucleotides corresponding to the target sequences were cloned into the pSuper.retro RNAi plasmid (Oligoengine). The targeting sequences are as following. Human PKACα: 5′-GGGTGATGCTGGTGAAACA-3′; Human PKACβ: 5′-GAAGAGTCATGTTGGTAAA-3′. The targeting sequences for both human PKACα and PKACβ (PKACs): #1: 5′-GAAGGTTCAGTGAGCCCCA-3′; #2: 5′-TAGCCAAAGCCAAAGAAGA-3′. siRNA oligonucleotides sequence for both mouse PKACα and PKACβ (PKACs): 5′-TAGCCAAAGCCAAAGAAGATT-3′.
Yan B.R., Zhou L., Hu M.M., Li M., Lin H., Yang Y., Wang Y.Y, & Shu H.B. (2017). PKACs attenuate innate antiviral response by phosphorylating VISA and priming it for MARCH5-mediated degradation. PLoS Pathogens, 13(9), e1006648.
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7

Silencing ZFYVE1 with RNAi Plasmid

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Double-strand oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro RNAi plasmid (Oligoengine). The following sequence of human ZFYVE1 mRNA was targeted: GCGGACAGAGATTGTGCAT.
Zhong X., Feng L., Zang R., Lei C.Q., Yang Q, & Shu H.B. (2020). ZFYVE1 negatively regulates MDA5- but not RIG-I-mediated innate antiviral response. PLoS Pathogens, 16(4), e1008457.
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8

Cloning of Targeted RNAi Plasmids

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Double-stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro-RNAi plasmid (Oligoengine). The following sequences were targeted for SPI-2 mRNA: #1 5′-GGAGAACATGGATAAGGTT-3′, #2 5′-GCGATATTCTGCCGTGTTT-3′. The sequence targeted by the control RNAi plasmid is 5′-GGAAGATGTATGGAGACATGG-3′.
Qin Y., Li M., Zhou S.L., Yin W., Bian Z, & Shu H.B. (2017). SPI-2/CrmA inhibits IFN-β induction by targeting TBK1/IKKε. Scientific Reports, 7, 10495.
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9

RNA Interference Targeting Viral Genes

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Double-stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper.Retro-RNAi plasmid (Oligoengine). The following sequences were targeted for UL42 mRNA: #1 5’-GCTGGTGGACCTCAACAACTT-3’; #2 5’-GCCAATGGATCATGCTGTTTC-3’; The following sequences were targeted for UL82: 5’-GCTGGTGGACCTCAACAACTT-3’; The following sequences were targeted for UL31: 5’-GGACAACTTTCTCACGTCT-3’; The sequence targeted by the control RNAi plasmid is: 5’-GGAAGATGTATGGAGACATGG-3’.
Fu Y.Z., Guo Y., Zou H.M., Su S., Wang S.Y., Yang Q., Luo M.H, & Wang Y.Y. (2019). Human cytomegalovirus protein UL42 antagonizes cGAS/MITA-mediated innate antiviral response. PLoS Pathogens, 15(5), e1007691.
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10

Targeting SNX8 and VPS34 with RNAi

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Double-stranded oligonucleotides corresponding to the target sequences were cloned into the pSuper-Retro RNAi plasmid (Oligoengine). The following sequences were targeted for human SNX8 cDNA: #1 (5′-CGGCAGATCTTCTCATATT-3′) and #2 (5′-CGGCAGATCTTCTCATATT-3′).
Human VPS34 cDNA: #1 (5′-GATCTGAAACCCAATGCTG-3′) and #2 (5′-GGAATGTGAAGATCAAGAT-3′).
Mouse Vps34 cDNA: (5′- GCTGTCCTAGAAGATCCCA-3′)
Wei J., Lian H., Guo W., Chen Y.D., Zhang X.N., Zang R., Zhong L., Yang Q., Hu M.M., Luo W.W., Shu H.B, & Li S. (2018). SNX8 modulates innate immune response to DNA virus by mediating trafficking and activation of MITA. PLoS Pathogens, 14(10), e1007336.
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