WT and Nox2−/− (Cybb−/−) female mice on the C57BL/6 background, and C3(1)-TAg mice are from The Jackson Laboratory. mNox2−/− mice were generated by crossing Cybbfl/fl mice with LysM-cre knock in mice (004781; The Jackson Laboratory) to generate LysM-cre+/−Cybbfl/fl and littermate control Cybbfl/fl mice as previously described (Coats et al., 2017 (link)). Mouse genotype was confirmed by PCR (see Table S1 for primers).
Lysm cre knock in mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
LysM-cre knock in mice are genetically modified mice that express the Cre recombinase enzyme under the control of the LysM (Lysozyme M) gene promoter. The LysM gene is primarily expressed in myeloid cells, including macrophages and neutrophils. This allows for the Cre-mediated gene deletion or modification specifically in these cell types.
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Lab products found in correlation
2 protocols using lysm cre knock in mice
1
Generating mNox2-Deficient Mice
2
Macrophage-Specific Atg7 Conditional KO Mice
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Previously described Atg7fl/fl mice [50 (link)] and LysMCre knockin mice, acquired from The Jackson Laboratory (Bar Harbor, ME, USA), were crossed to produce macrophage-specific Atg7 conditional KO mice, i.e., Atg7fl/fl-LysMCre+/−. Deletion of the Atg7 gene was confirmed by genotyping, and its expression was examined by western blot and qRT-PCR. Mice were kept in temperature-controlled housing under a 12-h light/12-h dark cycle (lights on at 8:00 AM and off at 8:00 PM) with free access to water and a normal chow diet (Purina Rodent Chow, 38057). For the HFD study, the normal chow diet was switched to a 60% HFD (Research Diets, D12492) when the mice were 6-8 weeks of age. All animal studies were conducted according to an approved protocol from the Institutional Animal Care and Use Committee of Asan Life Science Institute, Asan Medical Center, Seoul, Korea.
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