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Lago x

Manufactured by Spectral Instruments Imaging
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The Lago X is a high-performance spectrometer designed for laboratory applications. It features a compact and modular design, allowing for customization to meet specific research requirements. The Lago X provides accurate and reliable spectral measurements across a wide range of wavelengths, making it a versatile tool for various analytical applications.

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Lab products found in correlation

Aura imaging software, by Spectral Instruments Imaging (4 mentions) D luciferin luck 2g, by GoldBio (3 mentions) D luciferin, by Spectral Instruments Imaging (2 mentions) Rediject d luciferin bioluminescent substrate, by PerkinElmer (2 mentions) Ami htx, by Spectral Instruments Imaging (1 mentions) Ivis spectrum, by PerkinElmer (1 mentions) Be0061, by BioXCell (1 mentions) Amiview software, by Spectral Instruments Imaging (1 mentions) Sodium pyruvate, by Corning (1 mentions) Mem non essential amino acid, by Corning (1 mentions) Pmdlg prre, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions) Prsv rev, by Addgene (1 mentions) Effectene transfection reagent, by Qiagen (1 mentions) Plenti cmv puro luc, by Addgene (1 mentions) Female balb c mice, by Jackson ImmunoResearch (1 mentions) Dmlb microscope, by Leica (1 mentions) Dfc310 fx camera, by Leica (1 mentions) Clodronate liposome, by Liposoma (1 mentions) Aura software, by Spectral Instruments Imaging (1 mentions)

18 protocols using lago x

1

Bioluminescent Imaging of Mice

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NSGS mice were injected intraperitoneally with 150mg/kg D-luciferin (XenoLight). Ten minutes later, mice were anesthetized and imaged with Lago X (Spectral Instruments Imaging). Bioluminescent signals were quantified using Aura imaging software (Spectral Instruments Imaging). Total flux values were determined and are presented as photons (p)/second (sec).
Huang F., Sun J., Chen W., He X., Zhu Y., Dong H., Wang H., Li Z., Zhang L., Khaled S., Marcucci G., Huang J, & Li L. (2020). HDAC4 inhibition disrupts TET2 function in high-risk MDS and AML. Aging (Albany NY), 12(17), 16759-16774.
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2

Bioluminescent Wound Infection Model

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Ten-to-twelve-week-old male mice were anesthetized, shaved, and received two dorsal excisional wounds as described previously (20 (link)). Mice were inoculated with 40 μl of luminescent bacteria per wound at the indicated doses 24 hours after wounding, and control mice were inoculated with sterile PBS. Mice were imaged daily for luminescent signal on the IVIS Spectrum (Perkin Elmer), the Ami HTX (Spectral Instruments Imaging), or the Lago-X (Spectral Instruments Imaging) at the Stanford Center for Innovation in In Vivo Imaging daily before takedown. Additional details on the surgical procedure and wound processing can be found in the supplementary methods.
Sweere J.M., Van Belleghem J.D., Ishak H., Bach M.S., Popescu M., Sunkari V., Kaber G., Manasherob R., Suh G.A., Cao X., de Vries C.R., Lam D.N., Marshall P.L., Birakova M., Katznelson E., Lazzareschi D.V., Balaji S., Keswani S.G., Hawn T.R., Secor P.R, & Bollyky P.L. (2019). Bacteriophage trigger antiviral immunity and prevent clearance of bacterial infection. Science (New York, N.Y.), 363(6434), eaat9691.
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3

Evaluating NK Cell Immunotherapy in B-ALL

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1×106 P493-6 cells were luciferase-labeled and injected intravenously into 4–6 weeks NOD-SCIDIL-2Rγ−/− (NSG) mice. Engraftment of P493-6 cells was confirmed by bioluminescence imaging (BLI) using Lago-X (spectral instruments imaging). D-luciferin monosodium salt dissolved in PBS was injected intraperitoneally at 2.5 mg/mouse 12 min before BLI. Mice were kept under general anesthesia (3%–5% isoflurane) during BLI. After verifying B-ALL engraftment on day 9, mice were intravenously injected with either CRISPRa control or IL-15-producing NK-92 cells (7×106 NK cells per mouse). BLI was performed twice a week to measure disease progression.
Kumar A., Taghi Khani A., Duault C., Aramburo S., Sanchez Ortiz A., Lee S.J., Chan A., McDonald T., Huang M., Lacayo N.J., Sakamoto K.M., Yu J., Hurtz C., Carroll M., Tasian S.K., Ghoda L., Marcucci G., Gu Z., Rosen S.T., Armenian S., Izraeli S., Chen C.W., Caligiuri M.A., Forman S.J., Maecker H.T, & Swaminathan S. (2023). Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemia. Journal for Immunotherapy of Cancer, 11(5), e006649.
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4

Tracking A20-luc Leukemia in Murine BMT

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A20-luciferase (A20-luc), a BALB/c B-cell lymphoblastic leukemia cell line, was generously provided by Dr. Xue-Zhong Yu, MD (Medical University of South Carolina) and has been used previously in murine BMT studies.2 (link) A20-luc was cultured in RPMI 1640 (Hyclone SH30027) with 10% FBS, MEM, and sodium pyruvate (Hyclone SH30239) at 37°C and 5% CO2 and administered iv (0.1x106) on day 0, with the BMT. To image tumor burden, A20-luc bearing mice were given luciferin (GoldBio; LUCK) ip 0.15 mg/g, anesthetized with isoflurane (Piramal Critical Care; 440532079), and imaged using a LagoX (Spectral Instruments Imaging, Tucson, AZ, USA). Luminescence was quantified using AmiView software (Spectral Instruments Imaging) and presented as ln(photons/second). For depletion experiments, injections of depletion antibodies (NK1.1, BE0036; GK1.5, BE0003-1; 2.43, BE0061) were given ip 200 μg weekly beginning on day +3 (Bio X Cell).
Stokes J., Hoffman E.A., Molina M.S., Kummet N., Simpson R.J., Zeng Y, & Katsanis E. (2020). Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia. Oncoimmunology, 9(1), 1758011.
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5

Bioluminescent Murine B-cell Leukemia Model

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A20, a BALB/c B-cell lymphoblastic leukaemia cell line (American Type Culture Collection, Manassas, VA), is radio-resistant and has been used previously in murine BMT studies(Chen, et al 2006 (link), Zeng, et al 2014 (link)). A20 was cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS), MEM nonessential amino acids, and sodium pyruvate (Cellgro, Manassas, VA) at 37°C and 5% CO2. Luciferase-expressing A20 cells were generously provided by Dr. Xue-Zhong Yu, MD (Medical University of South Carolina, Charleston, SC). At various time points, A20-Luc bearing mice were given luciferin i.p. 0.15 mg/g, anesthetized with isoflurane and imaged using a LagoX (Spectral Instruments Imaging, Tucson, AZ).
Stokes J., Hoffman E.A., Zeng Y., Larmonier N, & Katsanis E. (2016). Post-transplant bendamustine reduces GvHD while preserving GvL in experimental haploidentical bone marrow transplantation. British journal of haematology, 174(1), 102-116.
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6

Lentiviral Transduction and In Vivo Bioluminescence Imaging

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Prior to in vivo bioluminescence imaging, 3rd generation luciferase expression lentivirus was generated by co-transfection of 1.5 μg pLenti CMV Puro LUC plasmid (17477, Addgene) (Campeau et al., 2009 (link)), 0.5 μg pMD2.G (12259, Addgene), 0.3 μg pMDLg/PRRE (12251, Addgene), 0.7 μg pRSV-Rev (12253, Addgene) into HEK293T cells in 60 mm cell culture dish with Effectene Transfection Reagent (301427, QIAGEN), and then the leukemia cells or the tumor cells were infected with lentivirus and selected with 1 μg/ml puromycin to stably express luciferase. For in vivo bioluminescence imaging, the mice were weighed, injected intraperitoneally with 150 mg/kg D-luciferin (LUCK-2G, Goldbio) in PBS solution, and then anesthetized with isoflurane. The mice were imaged 10 minutes after D-luciferin injection with Lago X (Spectral Instruments Imaging). The bioluminescent signals were quantified using Aura imaging software (Spectral Instruments Imaging). Total flux values were determined by drawing regions of interest, which are identical among the mice in different group, and are presented in photons/second/cm2/steradian.
Su R., Dong L., Li Y., Gao M., Han L., Wunderlich M., Deng X., Li H., Huang Y., Gao L., Li C., Zhao Z., Robinson S., Tan B., Qing Y., Qin X., Prince E., Xie J., Qin H., Li W., Shen C., Sun J., Kulkarni P., Weng H., Huang H., Chen Z., Zhang B., Wu X., Olsen M.J., Müschen M., Marcucci G., Salgia R., Li L., Fathi A.T., Li Z., Mulloy J.C., Wei M., Horne D, & Chen J. (2020). Targeting FTO suppresses cancer stem cell maintenance and immune evasion. Cancer cell, 38(1), 79-96.e11.
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7

Lentiviral Knockdown in Xenograft Mouse Model

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MONO-MAC-6 cells were lentivirally infected with pLenti CMV Puro LUC (17477, Addgene) by two rounds of ‘spinoculation’ and selected with puromycin for 7 days. Then, the luciferase-labeled MONO-MAC-6 cells were infected with lentivirus to knock down FTO, PFKP, or LDHB prior to xenograft transplantation. For in vivo bioluminescence imaging, NRGS mice were weighted, injected intraperitoneally with D-luciferin (LUCK-2G, Goldbio) at 150 mg/kg, and anesthetized with isoflurane. The mice were imaged using a Lago X (Spectral Instruments Imaging) 10 minutes post D-luciferin injection and Aura imaging software (Spectral Instruments Imaging) was used to quantify the bioluminescent signals. Radiance is in the unit of “photons/second/cm2/steradian”.
Qing Y., Dong L., Gao L., Li C., Li Y., Han L., Prince E., Tan B., Deng X., Wetzel C., Shen C., Gao M., Chen Z., Li W., Zhang B., Braas D., ten Hoeve J., Sanchez G.J., Chen H., Chan L.N., Chen C.W., Ann D., Jiang L., Müschen M., Marcucci G., Plas D.R., Li Z., Su R, & Chen J. (2021). R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular cell, 81(5), 922-939.e9.
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8

Bioluminescent Assessment of Gene Transduction

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Female BALB/c mice, aged 12 weeks ± 1 week at the time of transduction, were sourced from The Jackson Laboratory. Mice were anesthetized with isoflurane and injected in the medial aspect of the right gastrocnemius muscle with 109 vector genomes. Because insulin concentration exhibits diurnal variation,48 (link),64 (link) mice were injected with vectors in the early afternoon, which is the midpoint of their daily photocycle. Transduction efficiency was assessed at the indicated days post transduction by measuring luciferase activity with RediJect D-Luciferin Bioluminescent Substrate (PerkinElmer) in a Lago X instrument (Spectral Instruments Imaging). To eliminate erroneous measurements from mice that were mishandled by, e.g., suboptimal luciferase substrate injection, mice with luminescence values differing by more than 3-fold from one time point to the next were excluded from analyses.
Detailed descriptions of materials and methods are available in the Supplemental information.
Jackson C.B., Richard A.S., Ojha A., Conkright K.A., Trimarchi J.M., Bailey C.C., Alpert M.D., Kay M.A., Farzan M, & Choe H. (2020). AAV vectors engineered to target insulin receptor greatly enhance intramuscular gene delivery. Molecular Therapy. Methods & Clinical Development, 19, 496-506.
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9

Establishing Brain Metastases in Mice

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All animal experiments were performed following protocols approved by the ethics committee of the Institutional Animal Care and Use Committee of Tongji Medical College, Huazhong University Science and Technology University. Male BALB/c nude mice (6–8 weeks of age) were used and randomly allocated to each group. For the brain metastases assay, first mice were anesthetized with 1.5% sodium pentobarbital, then 2 × 105 luciferase-labeled A549-BrM cells suspended in 100 µL of PBS were injected into the left cardiac ventricle of mice using 1ml syringe with a 26G needle, while the control group received 100 µL of PBS. To confirm successful brain metastases, mice were intraperitoneally injected with D-luciferin (150 mg/kg) and the photo flux from whole body was observed and imaged at 4 weeks after injection tumor cells by using the Lago X (Spectral Instruments Imaging, Tucson, AZ, USA) coupled with the Living Image Acquisition and Analysis software program (PerkinElmer, Waltham, MA, USA).
Chen P., Li Y., Liu R., Xie Y., Jin Y., Wang M., Yu Z., Wang W, & Luo X. (2022). Non–small cell lung cancer-derived exosomes promote proliferation, phagocytosis, and secretion of microglia via exosomal microRNA in the metastatic microenvironment. Translational Oncology, 27, 101594.
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10

Microscopic Imaging of Whole Mice

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Immunofluorescence images were taken using a Leica DMLB microscope and Leica DFC 310 FX camera mounted on a 1× C-mount using the LAS V4.5 software. Whole Mouse imaging was performed by the Arizona Cancer Imaging Shared Resource and used LagoX (Spectral Instruments Imaging) software-AMI View (v1.7.05).
Maisel S.A., Broka D., Atwell B., Bunch T., Kupp R., Singh S.K., Mehta S, & Schroeder J. (2019). Stapled EGFR peptide reduces inflammatory breast cancer and inhibits additional HER-driven models of cancer. Journal of Translational Medicine, 17, 201.
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