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Neuromedin b

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Neuromedin B is a peptide that functions as a neurotransmitter or neuromodulator in the nervous system. It is involved in various physiological processes, including regulation of smooth muscle contraction and secretion of hormones. Neuromedin B is commonly used in research applications to study its role in neurological and endocrine functions.

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Lab products found in correlation

Diphtheria toxin, by List Biological Laboratories (2 mentions) Anti fcεri antibody clone mar 1, by Thermo Fisher Scientific (2 mentions) Anti cd200r3 antibody clone ba103, by Hycult Biotech (2 mentions)

2 protocols using neuromedin b

1

Nippostrongylus brasiliensis Infection Model

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Methods for maintenance, recovery, infection and isolation of Nippostrongylus brasiliensis (Nb) larvae were performed as previously described53 . Mice were infected with 500 Nb larvae by subcutaneous injection. For basophil depletion, WT and basophil-depleted mice were treated with 0.375μg of diphtheria toxin (LIST biological laboratories, Cat. #150) i.p. every other day; mice were sacrificed 3–7 days post-Nb infection. Intestinal worm burdens were evaluated 7 days post-infection as described previously53 . For neuromedin B treatment, mice were anesthetized with isoflurane and then treated with 10μg of neuromedin B (MP Biomedicals) dissolved in 50 μL of PBS administered via intratracheal instillation. For antibody-mediated basophil depletion, Rag2–/– mice were treated with 20μg of anti-FcεRI〈 antibody (clone MAR-1, eBioscience) or anti-CD200R3 antibody (clone Ba103, Hycult Biotech) i.p. on days 1, 3, and 5 post-Nb infection.
Inclan-Rico J.M., Ponessa J.J., Valero-Pacheco N., Hernandez C.M., Sy C.B., Lemenze A.D., Beaulieu A.M, & Siracusa M.C. (2020). Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nature immunology, 21(10), 1181-1193.
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2

Nippostrongylus brasiliensis Infection Model

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Methods for maintenance, recovery, infection and isolation of Nippostrongylus brasiliensis (Nb) larvae were performed as previously described53 . Mice were infected with 500 Nb larvae by subcutaneous injection. For basophil depletion, WT and basophil-depleted mice were treated with 0.375μg of diphtheria toxin (LIST biological laboratories, Cat. #150) i.p. every other day; mice were sacrificed 3–7 days post-Nb infection. Intestinal worm burdens were evaluated 7 days post-infection as described previously53 . For neuromedin B treatment, mice were anesthetized with isoflurane and then treated with 10μg of neuromedin B (MP Biomedicals) dissolved in 50 μL of PBS administered via intratracheal instillation. For antibody-mediated basophil depletion, Rag2–/– mice were treated with 20μg of anti-FcεRI〈 antibody (clone MAR-1, eBioscience) or anti-CD200R3 antibody (clone Ba103, Hycult Biotech) i.p. on days 1, 3, and 5 post-Nb infection.
Inclan-Rico J.M., Ponessa J.J., Valero-Pacheco N., Hernandez C.M., Sy C.B., Lemenze A.D., Beaulieu A.M, & Siracusa M.C. (2020). Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nature immunology, 21(10), 1181-1193.
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