Dulbecco’s Modified Eagle Medium (DMEM) with high glucose was from Invitrogen. The HCC827, H292, H4006, 293T cell lines were purchased from American Type Culture Collection. Enzastaurin and erlotinib were from LC Laboratories (Woburn, MA). Go6976 and U0126 were purchased from EMD Millipore (Billerica, MA). Anti-phospho-p70S6K (Thr389), S6K, mTOR, phospho- Erk½ (Thr202/Tyr204), Erk½, (E747-A750del Specific) EGFR, EGFR, P-Akt (Ser473), P-Akt (Thr308), Akt, PKCα and ErbB3 antibodies were from Cell Signaling Technology (Danvers, MA), and anti-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti-EEA1 antibody was from BD Biosciences (San Jose, CA). siRNAs for PKCα and Gab1 were from Qiagen (Valencia, CA) and those for PLCγ1 were from ambion (life technologies). Dual mTOR inhibitor (KU-0063794) and Akt inhibitor (MK-2206 2HCl) were purchased from Selleckchem. All other reagents were from Sigma-Aldrich (St. Louis, MO).
Those for plcγ1
Manufactured by Thermo Fisher Scientific
The lab equipment for PLCγ1 is designed for the detection and analysis of PLCγ1 (Phospholipase C Gamma 1) in biological samples. PLCγ1 is an important enzyme involved in various cellular signaling pathways. The core function of this lab equipment is to provide researchers and scientists with the tools necessary to study and quantify the expression and activity of PLCγ1 in their research.
Automatically generated - may contain errors
Lab products found in correlation
Erbb3, by Cell Signaling Technology (2 mentions)
Dulbecco s modified eagle medium dmem with high glucose, by Thermo Fisher Scientific (2 mentions)
Go6976, by Merck Group (2 mentions)
Anti eea1 antibody, by BD (2 mentions)
Sirnas for pkcα and gab1, by Qiagen (2 mentions)
Dual mtor inhibitor ku 0063794, by Selleck Chemicals (2 mentions)
Akt inhibitor mk 2206 2hcl, by Selleck Chemicals (2 mentions)
Anti actin antibody, by Merck Group (2 mentions)
Erlotinib, by LC Laboratories (2 mentions)
Enzastaurin, by LC Laboratories (2 mentions)
Hcc827, by American Type Culture Collection (2 mentions)
U0126, by Merck Group (2 mentions)
H4006, by American Type Culture Collection (2 mentions)
2 protocols using those for plcγ1
1
Investigating EGFR Signaling Pathways
2
Investigating EGFR Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s Modified Eagle Medium (DMEM) with high glucose was from Invitrogen. The HCC827, H292, H4006, 293T cell lines were purchased from American Type Culture Collection. Enzastaurin and erlotinib were from LC Laboratories (Woburn, MA). Go6976 and U0126 were purchased from EMD Millipore (Billerica, MA). Anti-phospho-p70S6K (Thr389), S6K, mTOR, phospho- Erk½ (Thr202/Tyr204), Erk½, (E747-A750del Specific) EGFR, EGFR, P-Akt (Ser473), P-Akt (Thr308), Akt, PKCα and ErbB3 antibodies were from Cell Signaling Technology (Danvers, MA), and anti-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti-EEA1 antibody was from BD Biosciences (San Jose, CA). siRNAs for PKCα and Gab1 were from Qiagen (Valencia, CA) and those for PLCγ1 were from ambion (life technologies). Dual mTOR inhibitor (KU-0063794) and Akt inhibitor (MK-2206 2HCl) were purchased from Selleckchem. All other reagents were from Sigma-Aldrich (St. Louis, MO).
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