Mirabegron

Manufactured by Cayman Chemical

Sourced in United Kingdom

Mirabegron is a chemical reagent manufactured by Cayman Chemical. It is a selective agonist of the beta-3 adrenergic receptor, which is involved in the regulation of bladder function. The core function of Mirabegron is to serve as a research tool for studying the role of beta-3 adrenergic receptors in various biological processes.

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Lab products found in correlation

4 protocols using mirabegron

Preparation of Compound Stock Solutions

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100 mM stock solutions of all screened compounds were prepared in individual, sterile amber vials. All stock solutions were dissolved in sterile DMSO, except L-arginine, which was solubilized using dH₂O, due to solubility constraints. Sterile cell-culture grade DMSO, tamsulosin hydrochloride, nifedipine, isoproterenol, butoxamine, adenosine and L-arginine were obtained from Sigma Aldrich. PGE1, PGE2, rolipram, L-arginine, ondansetron, celecoxib, diclofenac, mirabegron, sildenafil, atropine, vardenafil and tiotropium were obtained from Cayman Chemical. Y-27632 dihydrochloride was obtained from R&D Systems. Stock solutions when unused were stored at −20 °C

Lee C.X., Cheah J.H., Soule C.K., Ding H., Whittaker C.A., Karhohs K., Burds A.A., Subramanyam K.S., Carpenter A.E., Eisner B.H, & Cima M.J. (2019). Identification and local delivery of vasodilators for the reduction of ureteral contractions. Nature biomedical engineering, 4(1), 28-39.

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Adrenergic Receptor Agonists: In Vivo, In Vitro, and Ex Vivo Applications

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The pan β-adrenergic agonist, isoproterenol hydrochloride
(ISO) was from Sigma; the β3-adrenoreceptor agonists, CL
316,243 disodium salt (CL) was obtained from Tocris Bioscience (UK)
and mirabegron (Mira) was from Cayman Chemical. For CL
administration in vivo, mice were acclimated to
thermoneutrality for 3 days, injected with 1 mg/kg CL, and euthanized for tissue
collection 60 minutes later. For β-adrenergic stimulation in
vitro, 3T3-L1 cells were treated with ISO (10 μM) for 40
minutes at 37⁰C. For β-adrenergic stimulation ex
vivo, human fat samples (approx. 0.5cm in size) were treated with
ISO (10 μM) for 40 minutes at 37⁰C. For β-adrenergic
stimulation in vivo, human subjects were administered Mira for
10 weeks (50 mg/day), with abdominal adipose biopsies before and after
treatment, as described previously (Finlin
et al., 2018).

Kasza I., Kühn J.P., Völzke H., Hernando D., Xu Y.G., Siebert J.W., Gibson A.L., Yen C.L., Nelson D.W., MacDougald O.A., Richardson N.E., Lamming D.W., Kern P.A, & Alexander C. (2021). Contrasting recruitment of skin-associated adipose depots during cold challenge of mouse and human. The Journal of physiology, 600(4), 847-868.

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Pharmacological Modulation of β3-Adrenoceptor

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CL316,243 was purchased from Tocris and dissolved in water. CL316,243 was administered at one dose (1 mg/Kg/day) for seven consecutive days by interperitoneal injections (IP). Mirabegron was purchased from Cayman Chemical and dissolved in water and was administered at one dose (1 mg/Kg/day) for seven consecutive days by IP. Talinolol was purchased from Cayman Chemical and was dissolved in DMSO. Administration solution was dissolved further in water (DMSO ~5%) and was administered at one dose (1 mg/Kg/day) for five consecutive days by IP. SR59230A was purchased from Sigma-Aldrich and was dissolved in DMSO. Administration solution was dissolved further in water (DMSO ~5%) and was administered at one dose (1 mg/Kg/day) for five consecutive days by IP.

Jiang Y., Berry D.C, & Graff J.M. (2017). Distinct cellular and molecular mechanisms for β3 adrenergic receptor-induced beige adipocyte formation. eLife, 6, e30329.

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Insulin and β-Adrenergic Modulation

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All cell culture consumables were from Thermo Fisher Scientific (Scoresby, Victoria, Australia). Human insulin (Actrapid) was obtained from Novo Nordisk. Mirabegron was obtained from Cayman Chemicals. CGP20712A, fatty‐free BSA, forskolin, ICI118551, (–)‐isoprenaline, and SR59230A were obtained from Sigma‐Aldrich. Rosiglitazone was obtained from Tocris Bioscience. All other reagents were of analytical quality.

Dehvari N., Sato M., Bokhari M.H., Kalinovich A., Ham S., Gao J., Nguyen H.T., Whiting L., Mukaida S., Merlin J., Chia L.Y., Wootten D., Summers R.J., Evans B.A., Bengtsson T, & Hutchinson D.S. (2020). The metabolic effects of mirabegron are mediated primarily by β3‐adrenoceptors. Pharmacology Research & Perspectives, 8(5), e00643.

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