Poly ethylene glycol diacrylate mn 575

Poly(ethylene glycol) diacrylate (M_n 575) was obtained commercially (Sigma Aldrich, St. Louis, MO) along with reduced glutathione (Chem-Impex International, Wood Dale, IL) and Irgacure 2959 (Ciba, Basel, Switzerland). Analytical grade meropenem (Supplementary Figure 1A) and vancomycin hydrochloride (Supplementary Figure 1B) were obtained from Sigma Aldrich (St. Louis, MO) along with pravastatin sodium hydrochloride (Supplementary Figure 1C) from Cayman Chemical (Ann Arbor, MI). Dulbecco’s modified phosphate buffered saline (PBS) without calcium and magnesium was obtained from Corning (Manassas, VA) and used for all loading and release experiments unless otherwise noted. Cation-adjusted Mueller-Hinton broth (CAMHB) and agar along with tryptic soy agar with 5% sheep’s blood plates were obtained commercially (Becton Dickinson, Franklin Lakes, NJ). Recombinant glutathione-S-transferase proteins were expressed and purified from Escherichia coli as previously described (27 (link)). Pseudomonas aeruginosa isolates (ATCC 27853 and AR 0440) were obtained from our internal biorepository and the Centers for Disease Control and Prevention (CDC) Antibiotic Resistance (AR) isolate bank (28 ).

Zinc nitrate hexahydrate (98%, Sigma-Aldrich), poly (ethylene glycol) diacrylate M_n = 575 (PEGda 575) (Sigma-Aldrich), 7-diethylamino-3-thenoylcoumarin (DETC) (Exciton), lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) (>95%, Sigma-Aldrich) and dimethyl sulfoxide (DMSO) (Sigma-Aldrich) were used as received without further purification.