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Microfine needle

Manufactured by BD
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Sourced in United Kingdom

The MicroFine needle is a laboratory equipment product designed for precision fluid handling and sampling. It features a fine, tapered tip to facilitate accurate and controlled liquid transfers. This product is intended for use in various scientific and research applications that require precise liquid manipulation.

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Lab products found in correlation

Anti il 17a clone 17f3, by BioXCell (1 mentions) Igg2a, by R&D Systems (1 mentions) Rtk2758, by BioLegend (1 mentions) Non immune igg control, by R&D Systems (1 mentions)

3 protocols using microfine needle

1

Cytokine Regulation of Bone Loss in CD18 Mice

Cited 1 time
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To determine cytokine involvement in naturally occurring bone loss in CD18−/− mice, anti-cytokine blocking antibodies, or their controls, were microinjected locally into the palatal gingiva (5 μg per site), three times weekly from the age of 4 to 18 weeks, using a 28.5-gauge MicroFine needle (BD Biosciences). Microinjections were performed on the mesial of the first molar and in the papillae between first and second and third molars on both sides of the maxilla [43 (link)]. The following anti-mouse antibodies were used: anti-IL-17A (clone 17F3) and IgG1 isotype control, both from BioXcell; anti-IL-12p35 (clone C18.2; eBioscience) and IgG2a isotype control (R&D Systems); anti-IL-12/IL-23p40 (clone C17.8) and IgG2a isotype control, both from R&D Systems; polyclonal anti-IL-23p19 and non-immune IgG control, both from R&D Systems; anti-mouse IL-6 (clone MP5-20F3), anti-TNF (clone MP6-XT22) and IgG1 isotype control, all three reagents from R&D Systems.
Kajikawa T., Wang B., Li X., Wang H., Chavakis T., Moutsopoulos N.M, & Hajishengallis G. (2020). Activation of metabolic nuclear receptors restores periodontal tissue homeostasis in mice with leukocyte adhesion deficiency-1. Journal of leukocyte biology, 108(5), 1501-1514.
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2

Murine Palatal Gingiva Microinjection

Cited 1 time
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A neutralizing mAb to IL-17A (rat IgG2a; M210) was generously provided by Amgen, and azide-free rat IgG2a (RTK2758; Biolegend) served as isotype control. Purified polyclonal goat IgG antibody to IL-23p19 subunit and non-immune IgG control were purchased from R&D Systems. The antibodies or their controls were microinjected locally into the murine palatal gingiva (5 μg per site), three times weekly, using a 28.5-gauge MicroFine needle (BD Biosciences). Microinjections were performed on the mesial of the first molar and in the papillae between first and second and third molars on both sides of the maxilla (19 (link)).
Moutsopoulos N., Konkel J., Sarmadi M., Eskan M.A., Wild T., Dutzan N., Abusleme L., Zenobia C., Hosur K.B., Abe T., Uzel G., Chen W., Chavakis T., Holland S.M, & Hajishengallis G. (2014). Defective Neutrophil Recruitment in Leukocyte Adhesion Deficiency Type I Disease Causes Local IL-17-driven Inflammatory Bone Loss. Science translational medicine, 6(229), 229ra40.
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3

Galleria mellonella Virulence Assay

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Similar sized G. mellonella larvae (Livefood Ltd, UK) were selected for use in experiments. All larval injections were performed in the last pro-leg using a 0.33 mm Micro-Fine needle (BD, UK). Groups of 10 larvae were infected with 6 × 103 conidia. Control groups of larvae were included in each experiment; 10 unmanipulated larvae and 10 larvae injected with 10 µl PBS. Larvae were incubated at 37 °C for 6 d. Larval death was characterised by lack of movement and melanisation (Gomez-Lopez et al., 2014 (link), Renwick et al., 2006 (link), Slater et al., 2011 (link)). Virulence assays were performed in duplicate.
Ballard E., Melchers W.J., Zoll J., Brown A.J., Verweij P.E, & Warris A. (2018). In-host microevolution of Aspergillus fumigatus: A phenotypic and genotypic analysis. Fungal Genetics and Biology, 113, 1-13.
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