Milli q gradient 10 water purification system

The reference substances of genistin, genistein, daidzin, and daidzein were commercially provided by Chengdu Must Biotechnology Co., Ltd. (Chengdu, China) with a purity ≥98% via UV-UHPLC. Their structures were fully elucidated by comparing the spectral data (ESI-MS and ¹H, ¹³C NMR). The kits of total cholesterol (TC), total triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), malondialdehyde (MDA), superoxide dismutase (SOD), and low-density lipoprotein cholesterol (LDL-C) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). In addition, the other reagents and solvents, which met the requirements of analytical experiments, were purchased from Beijing Chemical Works (Beijing, China). Simvastatin was supplied by Merck Pharmaceuticals Ltd (Hangzhou, China). Ultrapure water was derived from Milli-Q Gradient Å 10 water purification system (Millipore, Billerica, MA, USA).

Genistin, genistein, daidzin, daidzein, and puerain were commercially provided by Chengdu Must Biotechnology Co., Ltd. (Chengdu, Sichuan, China). These five reference standards with the purity higher than 98% were applicable to UHPLC-LTQ-Orbitrap analysis. Acetonitrile, methanol, and formic acid (HPLC grade) used in the mobile phase were obtained from Fisher Scientific (Fair Lawn, NJ, USA). In addition, the other reagents and solvents met the requirements of analytical experiments, which were from Beijing Chemical Works (Beijing, China). The ultrapure water was derived from Milli-Q Gradient Å 10 water purification system (Millipore, Billerica, MA, USA). Grace Pure^TM SPE C18-Low solid-phase extraction (SPE) cartridges (200 mg/3mL, 59 μm, 70 Å) for solid phase pretreatment of biological samples were supplied by Grace Davison Discovery Science™ (Deerfield, IL, USA).

The reference standard of phillyrin was purchased from Chengdu Must Biotechnology Co. Ltd. (Sichuan, China). After HPLC-UV analysis, the purity of phillyrin was not less than 98%. Acetonitrile, methanol, and formic acid (HPLC grade) used in the mobile phase were provided by Fisher Scientific (Fair Lawn, NJ, USA). C18-low solid-phase extraction (SPE) cartridges (3 L/60 g) for biological sample pretreatment were obtained from Waters (Milford, MA, USA). Ultrapure water was freshly prepared using the Milli-Q Gradient Å 10 water purification system (Millipore, Billerica, MA, USA). Moreover, other reagents and solvents met the requirements of analytical experiments in Beijing Chemical Works (Beijing, China).