Flow cytometry tubes

The following materials were used to process the sample:

15 mL tubes with strew cap (NerbePlus, ref. 02-502-8001. Winsen, Germany);

50 mL tubes with strew cap (NerbePlus, ref. 02-572-8001. Winsen, Germany);

5 mL Flow Cytometry tubes (Corning, ref. 352052. Corning, USA, New York);

70 μm tube filter (Biologix, ref. 15-1070. Jinan, Shandong, China);

20 μm tube filter (Pluriselect, ref. 43-10020. Leipzig, Alemania);

Scalpel Blades (Heinz Herenz, ref. 1110923. Hamburg, Germany);

Needle (BD Becton Dickinson, ref. 305899. Franklin Lakes, USA, New Jersey);

Syringe (BD Becton Dickinson, ref. 300928. Franklin Lakes, USA, New Jersey);

Sterile Petri dish (Deltalab, ref. 200219. Barcelona, Spain);

Sterile 5 mL Flow Cytometry tubes (Corning, ref. 352054. Corning, USA, New York,).

Falcon polystyrene adherent Petri dishes, 70 µm cell strainers, flow cytometry tubes, polypropylene conical tubes, and polystyrene tissue culture plates were purchased from Corning inc (Corning, NY, USA). CD11b microbeads for mice and LS columns, a QuadroMACS separator, and a MACS MultiStand platform were obtained from Miltenyi Biotec (Bergisch Gladbach, Germany). Chamber slides were from Thermo Fisher Scientific (Waltham, MA, USA). Leica DFC320, Leica DMIL (Leica, Wetzlar, Germany), and Nikon Eclispe TS2 (Nikon, Tokyo, Japan) microscopes and were used for standard light or immunofluorescence analysis. Flow cytometry experiments and cell sorting were performed, respectively, on a BD LSRFortessa or FACSAria (Becton Dickinson, Franklin Lakes, NJ, USA). The centrifuge was a Rotixa 50 RS (Hettich Zentrifugen, Tuttlingen, Germany) and a Multifuge X1R (Thermo Fisher scientific, Waltham, MA, USA).