Alexa flour 647 microscale protein labeling kit

The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips that with 19394 individual human GST-and His6-tagged full-length proteins. 1.5 μg/μl of protein that recombinant pUL138 purified from BL21/pET21a-UL138 was labeled with Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen). The data were extracted by GenePix Pro 6.0 from the microarray images. Background was defined as signals less than 20% of the maximal signal and removed from the subsequent analysis. The signal-to-noise ratios (SNR = F635 median/B635 median) were first calculated for all the spots to generate the candidate list of UL138 binding proteins. The SNR of a protein was averaged calculated from two duplicated spots.

The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6-tagged full-length proteins. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen). The data were extracted from the microarray images with GenePix ProTM 6.0. Background was defined as signals less than 20% of the maximum signal and removed from the subsequent analysis. The signal-to-noise ratios (SNR = F635 median/B635 median) were first calculated for all of the spots to generate the candidate list of US31-binding proteins. The SNR of a protein was calculated as the average of two duplicated spots.