Click it plus tunel alexa fluor 594 kit

Manufactured by Thermo Fisher Scientific

The Click-iT Plus TUNEL Alexa Fluor 594 kit is a detection reagent used for the identification and quantification of apoptotic cells. It utilizes a fluorescent Alexa Fluor 594 dye to label DNA fragments, providing a visual indication of cell death.

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Lab products found in correlation

Isolectin b4, by Vector Laboratories (2 mentions)

Close spelling variants of this product

Alexa Fluor 594 (2045 citations) Alexa 594 (538 citations) Alexa Fluors (21 citations) TUNEL kit (19 citations) Click-it Alexa Fluor 594 (8 citations) Click-iT® TUNEL Alexa Fluor® 594 (3 citations) PLUSTM (3 citations) Click‐iT TUNEL Alexa Fluor 594 Kit (2 citations)

2 protocols using click it plus tunel alexa fluor 594 kit

Quantifying Skeletal Muscle Vascular and Apoptotic Features

Cited 2 times

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For analysis of capillary and arteriole density, gastrocnemius muscle sections (7 μm) were stained with isolectin-B4 (Vector Laboratories, B-1205; 1:100) to identify endothelial cells and the smooth muscle marker, α-smooth muscle actin conjugated to Cy3 (Sigma-Aldrich, C6198, 1:100) as previously described^{19 (link)}. High resolution images were acquired (at × 200) and counts from 18 randomly selected fields were averaged and expressed as the number of capillaries and arterioles (< 50 μm) per mm² of muscular sections^{20 (link)}.
DNA fragmentation, associated with apoptosis, was detected in sections (7 μm) of gastrocnemius muscle using the commercially available terminal deoxynucleotidyl transferase mediated dUTP nick-end labelling (TUNEL) kit (Click-iT Plus TUNEL Alexa Fluor 594 kit, Life Technologies). Sections were counterstained with Isolectin B4 to identify endothelial cells and DAPI to label nuclei.
Skeletal tissue fibrosis was detected in transverse sections (7 µm) of gastrocnemius muscle using Mason’s Trichrome staining.

Neale J.P., Pearson J.T., Thomas K.N., Tsuchimochi H., Hosoda H., Kojima M., Sato T., Jones G.T., Denny A.P., Daniels L.J., Chandrasekera D., Liu P., van Rij A.M., Katare R, & Schwenke D.O. (2020). Dysregulation of ghrelin in diabetes impairs the vascular reparative response to hindlimb ischemia in a mouse model; clinical relevance to peripheral artery disease. Scientific Reports, 10, 13651.

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Investigating Skeletal Muscle Angiogenesis and Apoptosis

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For analysis of capillary and arteriole density, gastrocnemius muscle sections (7μm) were stained with isolectin-B4 (Vector Laboratories, B-1205; 1:100) to identify endothelial cells and the smooth muscle marker, a-smooth muscle actin conjugated to Cy3 (Sigma-Aldrich, C6198, 1:100) as previously described 20 . High resolution images were acquired (at x200) and counts from 18 randomly selected elds were averaged and expressed as the number of capillaries and arterioles (<50μm) per mm 2 of muscular sections 23 .
DNA fragmentation, associated with apoptosis, was detected in sections (7mm) of gastrocnemius muscle using the commercially available terminal deoxynucleotidyl transferase mediated dUTP nick-end labelling (TUNEL) kit (Click-iT Plus TUNEL Alexa Fluor 594 kit, Life Technologies). Sections were counterstained with Isolectin B4 to identify endothelial cells and DAPI to label nuclei.
Skeletal tissue brosis was detected in transverse sections (7 µm) of gastrocnemius muscle using Mason's Trichrome staining.

Neale J.P.H., Pearson J.T., Thomas K.N., Tsuchimouchi H., Hosoda H., Kojima M., Sato T., Jones G.T., Denny A., Daniels L.J., Chandrasekera D., Liu P., Uitterlinden A.G., Katare R., & Schwenke D.O. (2020). Dysregulation of ghrelin in diabetes impairs the vascular reparative response to hindlimb ischemia in a mouse model; clinical relevance to peripheral artery disease.

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