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Imaris software v9

Manufactured by Oxford Instruments
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Sourced in United Kingdom

Imaris software v9.3 is a multi-dimensional image analysis software for scientific research and life sciences applications. It provides tools for 3D and 4D visualization, segmentation, and quantification of microscopy data.

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Lab products found in correlation

Lsm780 fcs, by Zeiss (3 mentions) Prism v7, by GraphPad (1 mentions) Microsoft excel 2016, by GraphPad (1 mentions) Bitplane, by Oxford Instruments (1 mentions) Lsm800 gaasp, by Zeiss (1 mentions)

Spelling variants of this product

Imaris software (319 citations) Imaris (281 citations) Imaris v9.5 (21 citations)

4 protocols using imaris software v9

1

Image Analysis and Proteomics Workflow

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Image processing was performed using either the Fiji upgrade of ImageJ (58 (link)) or Imaris software v9.2 (Bitplane, Oxford Instruments). Quantifications for colocalization measurements were performed using Imaris software v9.2 (Bitplane, Oxford Instruments). Statistical analyses were performed with Microsoft Excel 2016 and Prism v7.04 (GraphPad). Flow cytometry analysis was done using FlowJo software v10.4.2 (FlowJo LLC). Raw MS data were first analyzed using MaxQuant v1.6.0.16. Differential proteomics data analysis was performed using DAPAR v1.10.3 and ProStaR v1.10.4.
Deffieu M.S., Cesonyte I., Delalande F., Boncompain G., Dorobantu C., Song E., Lucansky V., Hirschler A., Cianferani S., Perez F., Carapito C, & Gaudin R. (2021). Rab7-harboring vesicles are carriers of the transferrin receptor through the biosynthetic secretory pathway. Science Advances, 7(2), eaba7803.
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2

Quantitative Analysis of Neuroangiogenic Markers

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All images for quantification were obtained either with upright fluorescent microscopy (Leica DM6, Leica Microsystems Inc.) or confocal microscopy (Zeiss LSM780 FCS, Carl Zeiss Microscopy GmbH). NGF-eGFP reporter activity, TUBB3+ nerve fibers, CD31+ blood vessels, and OCN+ osteoblasts were quantified using either the magic wand tool of Photoshop CC, 2017 with a tolerance of 30 (Adobe, San Jose, CA) using five random 40x microscopical fields per sample or three-dimensional volumetric analysis of Imaris software v9.3 (Oxford Instruments, Belfast, UK) using eight serial fields per sample within the defect tissue.
Meyers C.A., Lee S., Sono T., Xu J., Negri S., Tian Y., Wang Y., Li Z., Miller S., Chang L., Gao Y., Minichiello L., Clemens T.L, & James A.W. (2020). A Neurotrophic Mechanism Directs Sensory Nerve Transit in Cranial Bone. Cell reports, 31(8), 107696.
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3

Quantitative Analysis of Neuroangiogenic Markers

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All images for quantification were obtained either with upright fluorescent microscopy (Leica DM6, Leica Microsystems Inc.) or confocal microscopy (Zeiss LSM780 FCS, Carl Zeiss Microscopy GmbH). NGF-eGFP reporter activity, TUBB3+ nerve fibers, CD31+ blood vessels, and OCN+ osteoblasts were quantified using either the magic wand tool of Photoshop CC, 2017 with a tolerance of 30 (Adobe, San Jose, CA) using five random 40x microscopical fields per sample or three-dimensional volumetric analysis of Imaris software v9.3 (Oxford Instruments, Belfast, UK) using eight serial fields per sample within the defect tissue.
Meyers C.A., Lee S., Sono T., Xu J., Negri S., Tian Y., Wang Y., Li Z., Miller S., Chang L., Gao Y., Minichiello L., Clemens T.L, & James A.W. (2020). A Neurotrophic Mechanism Directs Sensory Nerve Transit in Cranial Bone. Cell reports, 31(8), 107696.
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4

Quantification of Nerve Fibers and Vasculature

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Images for quantification were obtained with confocal microscopy (Zeiss LSM780 FCS, or LSM800 GaAsP, Carl Zeiss Microscopy GmbH). TUBB3+ nerve fibers, CD31+, Emcn+ and CD146+ blood vessels were quantified using three-dimensional volumetric analysis of Imaris software v9.3 (Oxford Instruments, Belfast, UK) using eight serial fields per sample within the injured tissue, which included the distal tenotomy site and immediately surrounding tissues. For quantification of angiogenic factors within the DRG, cross sections of the whole ganglia were used with Leica DM6 B (Wetzlar, Germany) imaging and ImageJ software.
Qin Q., Gomez-Salazar M., Cherief M., Pagani C.A., Lee S., Hwang C., Tower R.J., Onggo S., Sun Y., Piplani A., Li Z., Ramesh S., Clemens T.L., Levi B, & James A.W. (2022). Neuron-to-vessel signaling is a required feature of aberrant stem cell commitment after soft tissue trauma. Bone Research, 10, 43.
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