FT-IR spectra were recorded on a Bio-Rad FTS-185 spectrometer at room temperature in the wavenumbers ranging from 4000 to 500 cm−1 with 4 cm−1 spectral resolution (64 times scanning). Samples were dissolved in organic solvent (chloroform or pyridine) and casted on potassium bromide (KBr) pellets by evaporation of the solvent.
Fts 185 spectrometer
Manufactured by Bio-Rad
The FTS-185 spectrometer is a laboratory instrument designed for spectroscopic analysis. It is capable of measuring the absorption or emission spectra of various samples across a range of wavelengths. The core function of the FTS-185 is to provide accurate and reliable spectroscopic data for scientific research and applications.
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3 protocols using fts 185 spectrometer
1
FT-IR Spectroscopy of Organic Compounds
2
Polymer Characterization by Advanced Techniques
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The structures of monomer and polymers were characterized by 1H NMR spectrum on a Varian 400 FT-NMR spectrometer (400.0 MHz). FTIR spectra were recorded on a Bio-Rad FTS-185 spectrometer at room temperature. The molecular weight (Mn and Mw) and polydispersity index (Mw/Mn) of polymers were determined by gel permeation chromatography (GPC) with a Waters 2414 refractive index detector. A series of commercial polystyrene standards were used for calibration curves. The average particle size, size distribution and morphology of micelles were measured by dynamic light scattering (DLS, Malvern Zeta Sizer) and transmission electron microscopy (TEM).
3
Characterization of Glycopolymer Precursors
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NMR spectrum. 1 H NMR spectroscopy was performed on a Varian-300 FT-NMR spectrometer at 300.0 MHz for proton nuclei, and 13 C NMR was implemented on a Bruker Avance-400 NMR spectrometer, operating at 100.0 MHz for 13 C nuclei, tetramethylsilane (TMS) was used as an internal chemical shift reference for all NMR measurements.
FT-IR spectra. FT-IR spectra were recorded on a Bio-Rad FTS-185 spectrometer at room temperature in the wavelength ranging from 4000 to 500 cm À1 with 4 cm À1 spectral resolution (64 times scanned).
Gel permeation chromatography (GPC). Average molecular weights (M n and M w ) and polydispersity indices (M w /M n ) of the glycopolymer precursors were measured at 35 1C on a PerkinElmer 200 GPC equipped with a refractive index detector (RI). Tetrahydrofuran was utilized as the eluent at a flowing rate of 1.0 mL min À1 , and a series of commercial polystyrene standards (Polymer laboratories, UK) were applied to calibrate the GPC elution traces (Table 1).
FT-IR spectra. FT-IR spectra were recorded on a Bio-Rad FTS-185 spectrometer at room temperature in the wavelength ranging from 4000 to 500 cm À1 with 4 cm À1 spectral resolution (64 times scanned).
Gel permeation chromatography (GPC). Average molecular weights (M n and M w ) and polydispersity indices (M w /M n ) of the glycopolymer precursors were measured at 35 1C on a PerkinElmer 200 GPC equipped with a refractive index detector (RI). Tetrahydrofuran was utilized as the eluent at a flowing rate of 1.0 mL min À1 , and a series of commercial polystyrene standards (Polymer laboratories, UK) were applied to calibrate the GPC elution traces (Table 1).
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