(R,S)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid ((±)-CPP, Alomone Labs, C-175) and 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTEP hydrochloride, Abcam, ab120035) were prepared in distilled water and diluted with saline to the required concentration. Trans-N,N′-(Cyclohexane-1,4-diyl)bis(2-(4-chlorophenoxy) acetamide (ISRIB, Sigma, SML0843) was dissolved in dimethyl sulfoxide (DMSO) with gentle warming in a 40°C water bath and vortexed until the solution became clear. Then the solution was diluted in polyethylene glycol 400 (PEG400) with gentle warming in a 40°C water bath and vortexed. The solution was prepared freshly and diluted in warm saline (37°C) before injection. 1:1 DMSO and PEG400 in saline was used as vehicle control. The choice of dose and timing of ISRIB administration was based on previous reports [42 (link)] and our study of the pharmacokinetics of ISRIB in live rats [44 (link)]. Four pair-housed aged (17-18-month-old) rats received a single daily injection of ISRIB (2.5 mg/kg, i.p.) on 3 consecutive days. Then the rats stayed pair-housed for 18 days after the third injection of ISRIB.
Ab120035
Manufactured by Abcam
Sourced in United States
Ab120035 is a laboratory equipment product offered by Abcam. It is a device designed for scientific research purposes. The core function of this product is to facilitate specific tasks or experiments within a laboratory setting. No further details about the intended use or interpretation of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab120035
1
ISRIB Enhances Cognitive Function in Aged Rats
2
mGluR5 Modulation in SMB-S15 and SMB-PS Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SMB-S15 cells were exposed to 100 µM MTEP (a selective mGluR5 antagonist, ab120035, Abcam, United States) for 30 min, and then stimulated by 100 μM L-glutamate (ab120049, Abcam, United States) for 1 h; SMB-S15 cells were exposed to 100 μM L-Glutamate for 1 h, and then treated by 100 µM MTEP for 1 h; SMB-PS cells were exposed to 100 μM CHPG (a selective mGluR5 agonist, ab120221, Abcam, United States) for 1 h. Various treated cells were harvested for further study.
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