The following antibodies were used: horseradish peroxidase (HRP)-conjugated anti-penta-His mouse monoclonal antibody (Qiagen GmnH, Hilden, Germany), anti-NCL mouse monoclonal antibody (Santa Cruz Biotechnology Inc., Dallas, TX, USA), anti-PARP rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA), anti-actin rabbit polyclonal antibody (Sigma-Aldrich, St Louis, MO, USA), HRP-conjugated anti-rabbit immunoglobulins from goat antiserum, HRP-conjugated anti-mouse immunoglobulins from goat antiserum (Thermo Scientific, Rockford, IL, USA).
Horseradish peroxidase
Manufactured by Qiagen
Sourced in Germany
Horseradish peroxidase is an enzyme commonly used in various laboratory applications. It catalyzes the oxidation of substrates in the presence of hydrogen peroxide. The core function of horseradish peroxidase is to serve as a sensitive and versatile reporter molecule in various biochemical and immunological assays.
Automatically generated - may contain errors
2 protocols using horseradish peroxidase
1
Antibody Characterization Protocol
2
Western Blot Analysis of ACR and ADO
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Expression of the ACR and ADO genes in transformed C. thermocellum strains was analyzed by western blotting. The transformed C. thermocellum strains were cultured in CTFUD with 24 μg mL -1 of thiamphenicol at 55 • C (Olson and Lynd 2012a). The cells were lysed and the proteins were resolved by SDS-PAGE and electrotransferred onto PVDF membranes using a Com-pactBLOT apparatus (ATTO). ACR and ADO were detected on the membranes using an anti-His-tag antibody conjugated with horseradish peroxidase (QIAGEN) and ECL Western Blotting Detection Regents (GE Healthcare Life Sciences).
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