Anti ocn
Anti-OCN is an antibody used to detect the presence of osteocalcin (OCN), a protein secreted by osteoblasts and found in bone. This antibody can be used in various laboratory techniques to measure OCN levels, which are important for studying bone metabolism and mineralization processes.
Lab products found in correlation
17 protocols using anti ocn
Immunohistochemical Analysis of Bone Markers
Histological Analysis of Murine Femur Tissues
Histological and Immunohistochemical Analysis of Dental Extracellular Matrix Proteins
For immunohistochemistry, deparaffinized sections were rehydrated and subjected to antigen retrieval at 37°C for 30 min. The samples were protein blocked with a serum‐free blocking solution at room temperature for 1 h, then, incubated with anti‐OPN (1:100 dilution, Abcam, MA, USA), anti‐OCN (1:50 dilution, Santa Cruz, CA, USA), anti‐DSPP (1:50 dilution, Santa Cruz, CA, USA), and ani‐DMP‐1 (1:500 dilution, Santa Cruz, CA, USA) primary antibodies at 4°C overnight. The samples were incubated with the secondary biotinylated anti‐goat antibody (BA‐5000, Vector Laboratories) for 30 min. Streptavidin‐conjugated horseradish peroxidase (SA‐5704, Vector Laboratories) was added for 30 min, and the samples were stained with 3,3′‐Diaminobenzidine (DAB). Then, Gill's hematoxylin was used to counterstain the cell nuclei. The stained sections were visualized under the light microscope and the positive areas of OPN, OCN, DSPP, and DMP‐1 were measured using Image J software.
Comprehensive Protein Profiling Protocol
Femur Characterization via Histological Analysis
Western Blot Analysis of Osteogenic Markers
Western Blot Analysis of Osteogenic Markers
Osteoblast Differentiation Protein Analysis
Quantitative Western Blot Analysis of Osteogenic Markers
0.1% Tween 20-Tris-buffered saline (TBST) containing 5% skim milk, membranes were
incubated overnight at 4°C with anti-ALP (diluted 1:200; Santa Cruz Biotechnology),
anti-COL-1 (diluted 1:500; Abcam, Cambridge, UK), anti-OCN (diluted 1:200; Santa Cruz
Biotechnology), anti-OPN (diluted 1:1,000; Abcam), anti-YAP (diluted 1:1,000; Abcam) and
GAPDH antibody (diluted 1:200; Zsjq Bio Co., China). Following three washes with TBST,
membranes were incubated for 1 h with horseradish peroxidase-conjugated secondary antibody
(diluted 1:2,000; Zsjq Bio Co., Beijing, China). Reactive bands were visualized using an
enhanced chemiluminescence kit (Millipore). The densities of the bands were computer
analyzed by a densitometer (Quantity One, Bio-Rad).
Immunohistochemical Analysis of Bone Markers
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