Log In Sign up
The largest database of trusted experimental protocols

Amersham alkphos direct labelling

Manufactured by GE Healthcare
Visit Supplier
Sourced in United States

The Amersham AlkPhos Direct Labelling is a lab equipment product designed for direct labeling of nucleic acids. It provides a simple and efficient method for the incorporation of alkaline phosphatase into DNA or RNA probes.

Automatically generated - may contain errors

Lab products found in correlation

Cdp star detection reagent, by GE Healthcare (3 mentions) Miracloth filter, by Merck Group (1 mentions)

Spelling variants of this product

Amersham Gene Images AlkPhos Direct Labelling and Detection System (5 citations)

3 protocols using amersham alkphos direct labelling

1

Genomic DNA Isolation and Southern Hybridization

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For isolation of genomic DNA, V. dahliae strains were grown with shaking at 120 rpm and 25 °C in liquid PDM. Mycelia were harvested using Miracloth and ground to powder in liquid nitrogen. Genomic DNA was isolated with phenol as described previously [42 (link)]. Southern hybridizations were performed as described [40 (link),42 (link)] using Amersham AlkPhos Direct Labelling and CDP-Star detection reagents (GE Healthcare, Chicago, IL, USA).
Maurus I., Leonard M., Nagel A., Starke J., Kronstad J.W., Harting R, & Braus G.H. (2022). Tomato Xylem Sap Hydrophobins Vdh4 and Vdh5 Are Important for Late Stages of Verticillium dahliae Plant Infection. Journal of Fungi, 8(12), 1252.
+ Open protocol
+ Expand
2

Fungal Genomic DNA Extraction and Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA extraction and Southern hybridization were performed as previously described [14 (link),17 (link)]. Briefly, mycelia were harvested using Miracloth filters and homogenized into powder in liquid nitrogen. Genomic DNA was isolated with phenol from mycelium grown shaking at 25°C in liquid PDM. Amersham AlkPhos Direct Labelling and CDP-Star detection reagents (GE Healthcare) were used for Southern hybridization.
Maurus I., Harting R., Herrfurth C., Starke J., Nagel A., Mohnike L., Chen Y.Y., Schmitt K., Bastakis E., Süß M.T., Leonard M., Heimel K., Valerius O., Feussner I., Kronstad J.W, & Braus G.H. (2023). Verticillium dahliae Vta3 promotes ELV1 virulence factor gene expression in xylem sap, but tames Mtf1-mediated late stages of fungus-plant interactions and microsclerotia formation. PLOS Pathogens, 19(1), e1011100.
+ Open protocol
+ Expand
3

Genomic DNA Extraction and Southern Hybridization

Check if the same lab product or an alternative is used in the 5 most similar protocols
For mycelium production strains were grown in PDM at 25°C. Mycelia were harvested using Miracloth filters (Calbiochem Merck) and ground in liquid nitrogen. Genomic DNA was extracted using phenol. Southern hybridization was carried out using the Amersham AlkPhos Direct Labelling and CDP-Star Detection reagents (GE Healthcare). Further details can be found in previous publications [56 (link),58 (link),59 (link)].
Höfer A.M., Harting R., Aßmann N.F., Gerke J., Schmitt K., Starke J., Bayram Ö., Tran V.T., Valerius O., Braus-Stromeyer S.A, & Braus G.H. (2021). The velvet protein Vel1 controls initial plant root colonization and conidia formation for xylem distribution in Verticillium wilt. PLoS Genetics, 17(3), e1009434.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!