Infinite m1000 pro spectrophotometer

Manufactured by Tecan

Sourced in Austria

The Infinite M1000 PRO spectrophotometer is a high-performance instrument designed for precise and accurate absorbance measurements. It features a wide wavelength range, advanced optics, and versatile accessories to support a variety of applications in life science research and analytical laboratories.

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Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (2 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Quartz cuvette, by Thermo Fisher Scientific (1 mentions) 50 bio uv visible spectrophotometer, by Agilent Technologies (1 mentions) Lipofectamine 8000, by Beyotime (1 mentions) Pex 3, by GenePharma (1 mentions) Zetasizer nano zs device, by Malvern Panalytical (1 mentions) 96 deepwell plate, by Eppendorf (1 mentions) Costar 96 well assay plate, by Corning (1 mentions)

Spelling variants of this product

Infinite M1000 Pro (823 citations) Infinite M1000 (648 citations) M1000 (189 citations) M1000 Pro (123 citations) Infinite M1000 spectrophotometer (8 citations) M1000 spectrophotometer (5 citations) Infinite Pro spectrophotometer (3 citations)

5 protocols using infinite m1000 pro spectrophotometer

Nanoparticle Characterization by Light Scattering

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The hydrodynamic diameter and ζ-potential measurements were performed by dynamic and electrophoretic light scattering, respectively, using a Zetasizer Nano ZS (Malvern Instruments, UK) device. Number weighted size distributions were used for analysis. ζ-potential measurements were performed in a 10 mM NaCl solution using the Smoluchowski approximation.
Extinction spectra of aqueous colloids of nanoparticles were recorded by an Infinite M1000 PRO spectrophotometer (Tecan, Austria).

Pastukhov A.I., Belyaev I.B., Bulmahn J.C., Zelepukin I.V., Popov A.A., Zavestovskaya I.N., Klimentov S.M., Deyev S.M., Prasad P.N, & Kabashin A.V. (2022). Laser-ablative aqueous synthesis and characterization of elemental boron nanoparticles for biomedical applications. Scientific Reports, 12, 9129.

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Spectroscopic Characterization of Proteins and Metabolites

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All absorption spectra were acquired in a Cary 50 Bio UV–Visible spectrophotometer. Each purified protein or metabolite was diluted to 20 μM in a buffer containing 150 mM NaCl and 50 mM Tris–HCl pH 7.4. Samples were placed in a Fisher brand quartz cuvette, and the absorbance was collected from 600 to 200 nm on the slowest scan speed.
Fluorescence emission spectra were collected on a Tecan Infinite M1000 PRO spectrophotometer. For native fluorescence, each protein was diluted to 20 μM and each metabolite was diluted to 2 μM in a buffer of 150 mM NaCl and 50 mM Tris–HCl pH 7.4. Samples were excited at 450 nm with a 5 nm bandwidth. Emission data was collected from 500 to 600 nm in 1 nm steps with a 5 nm bandwidth. All samples were measured in triplicate and plotted with Datagraph as the mean value ± standard deviation. For denatured fluorescence, each protein and metabolite were diluted as above (20 μM and 2 μM, respectively) plus 7 M guanidine hydrochloride (GuHCl). Measurements, parameters, and plotting were identical for the both the denatured and native fluorescence samples.

LaFrance B.J., Cassidy-Amstutz C., Nichols R.J., Oltrogge L.M., Nogales E, & Savage D.F. (2021). The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein. Scientific Reports, 11, 22810.

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HUVEC-Mediated HepG2 Cell Viability Assay

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HUVECs were cultured in 6-well flat-bottom plates at a density of 2.8 × 10⁵ cells/well, and the culture medium was replaced with fresh DMEM complete medium after 24 h. Then, 5 μL of Lipofectamine 8000 (Beyotime Biotechnology Inc., Shanghai, China), 125 μL of OptiMEM (Thermo Fisher Scientific), and 2.5 μg of plasmid pEX-3 with or without specific gene cDNA (GenePharma Ltd., Shanghai, China) were mixed homogeneously, left for 5 min, and added to the 6-well flat-bottom plates. After 4 h of incubation, the medium was replaced with fresh DMEM complete medium. The culture supernatant of HUVECs was collected after 48 h of incubation, during which time the plasmid showed good transfection efficiency in HUVECs.
The HepG2 cells were cultured in 12-well, flat-bottom plates at a density of 2 × 10⁵ cells/well. After 24 h of incubation, the culture medium was replaced with the supernatants collected from HUVECs, and 20 mM APAP was added. The culture supernatant of another empty vector plasmid, pEX-3, was used as the control. After 24 h, the viability of HepG2 cells was examined using the Cell Counting Kit-8 assay. The absorbance of the solution was measured at 450 nm using an Infinite M1000 Pro spectrophotometer (TECAN Ltd., Männedorf, Germany). The absorbance of the empty vector plasmid pEX-3 control was set as 100% cell viability.

Yu L., Yan J., Zhan Y., Li A., Zhu L., Qian J., Zhou F., Lu X, & Fan X. (2023). Single-cell RNA sequencing reveals the dynamics of hepatic non-parenchymal cells in autoprotection against acetaminophen-induced hepatotoxicity. Journal of Pharmaceutical Analysis, 13(8), 926-941.

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Characterization of Nanoparticle Properties

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Nanoparticle size and morphology were analyzed by high‐resolution transmission electron microscopy with Titan Themis Z microscope (Thermo Fisher Scientific, Netherlands) coupled with Super‐X energy dispersive X‐ray detector. Degradation of nanoparticles was analyzed by scanning electron microscopy using Tescan MAIA 3 device (Tescan, Czech Republic) and X‐act energy dispersive detector (Oxford Instruments, High Wycombe, UK) at 25 kV accelerating voltage.
Raman spectra were measured by a Confotec MR350 confocal micro‐Raman spectrometer (SOL instruments, Belarus) under excitation with 633‐nm laser. UV–vis extinction spectra were recorded by Infinite M1000 PRO spectrophotometer (Tecan, Austria).
The hydrodynamic diameter and ζ‐potential of nanoparticles were measured by dynamic and electrophoretic light scattering with a Zetasizer Nano ZS device (Malvern Instruments, UK). Number‐size distribution was used for hydrodynamic size analysis. To measure ζ‐potential nanoparticles were incubated in 10 mm NaCl for 15 min, mobility was derived with Smoluchowski approximation.

Belyaev I.B., Zelepukin I.V., Kotelnikova P.A., Tikhonowski G.V., Popov A.A., Kapitannikova A.Y., Barman J., Kopylov A.N., Bratashov D.N., Prikhozhdenko E.S., Kabashin A.V., Deyev S.M, & Zvyagin A.V. (2024). Laser‐Synthesized Germanium Nanoparticles as Biodegradable Material for Near‐Infrared Photoacoustic Imaging and Cancer Phototherapy. Advanced Science, 11(20), 2307060.

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High-throughput Bacterial Growth Measurement

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To facilitate high-throughput cell growth measurements, three individual colonies corresponding to each construct were seeded into 96-deep well plates (Eppendorf) where each well contained 100 μL LB media. Culture plates were then sealed using plate sealer and placed in an incubator shaker at 37 °C for 16 h. Then, 5 μL of the overnight culture was subcultured into fresh 100 μL LB media and incubated for 8 h, after which IPTG was supplemented to a final concentration of 0.1 mM. Protein expression proceeded at 16 °C for 18 h. To measure OD₆₀₀, 10 μL of each sample was mixed with 90 μL DI water in a Costar 96-well assay plate (Corning) and OD₆₀₀ of all samples was measured in an Infinite M1000Pro spectrophotometer (Tecan).

Jaroentomeechai T., Kwon Y.H., Liu Y., Young O., Bhawal R., Wilson J.D., Li M., Chapla D.G., Moremen K.W., Jewett M.C., Mizrachi D, & DeLisa M.P. (2022). A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nature Communications, 13, 6325.

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