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Cd14 qdot605 tuk4

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CD14 Qdot605 (Tuk4) is a fluorescently-labeled antibody that binds to the CD14 antigen on the surface of monocytes and macrophages. It is used for the identification and analysis of these cell types in flow cytometry applications.

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Lab products found in correlation

Cellsdirect 2x reaction mix, by Thermo Fisher Scientific (2 mentions) Cd3 alexa 700 sp34 2, by BD (2 mentions) Hla dr pe cy7 g46 6, by BD (2 mentions) Cd45ra ecd 3p, by Beckman Coulter (2 mentions) Cd27 apc efluor780 o323, by Thermo Fisher Scientific (2 mentions) Cd8 pe sk1, by BD (2 mentions) Amine aqua amcyan, by Thermo Fisher Scientific (2 mentions) Rnaqueous micro lysis buffer, by Thermo Fisher Scientific (2 mentions) Cd16 fitc 3g8, by BD (2 mentions) Cd4 qdot655 s3, by Thermo Fisher Scientific (2 mentions) Facsaria, by BD (2 mentions) Aqua viability dye, by Thermo Fisher Scientific (1 mentions) Cd3 v450 ucht1, by Thermo Fisher Scientific (1 mentions) Cd16 pacific orange 3g8, by Thermo Fisher Scientific (1 mentions) Cd89 pe a59, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD14 Qdot605 (clone Tuk4) CD14 (Tuk4;

3 protocols using cd14 qdot605 tuk4

1

Multicolor Flow Cytometry Cell Sorting

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Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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2

Multicolor Flow Cytometry of Fc Receptors

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MDM, MDDC, or PBMC were stained using a multicolored flow cytometry panel designed to determine Fc receptor expression. Cells were stained with CD3 V450 (UCHT1), CD14 Qdot 605 (T[u]K4) (Invitrogen), CD16 Pacific Orange (3G8) (Invitrogen), CD11c A700 (B-ly6), CD123 phycoerythrin (PE)-Cy5 (9F5), CD32 allophycocyanin (APC) (FLI8.26), CD64 APC H7 (10.1), CD89 PE (A59), and CD19 fluorescein isothiocyanate (FITC) (HIB19). Unless otherwise specified, all antibodies were sourced from BD Biosciences. Dead cells were excluded from analysis through staining with Aqua viability dye (Invitrogen).
Cheeseman H.M., Olejniczak N.J., Rogers P.M., Evans A.B., King D.F., Ziprin P., Liao H.X., Haynes B.F, & Shattock R.J. (2016). Broadly Neutralizing Antibodies Display Potential for Prevention of HIV-1 Infection of Mucosal Tissue Superior to That of Nonneutralizing Antibodies. Journal of Virology, 91(1), e01762-16.
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3

Multicolor Flow Cytometry Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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