Isotype control antibody clone mopc 173

Anti-mouse CD45.2 antibodies conjugated to APC, FITC, or Alexa 594 (clone 104, cat # 109814, 109805, or 109850, respectively; Biolegend) were injected at concentrations of 0.04, 0.4, or $2\mathrm{mg}/\mathrm{kg}$ in a total volume of 50 to $100\mu \mathrm{L}$ . Isotype control antibody (clone MOPC-173, cat # 400207; Biolegend) was used at $0.4\mathrm{mg}/\mathrm{kg}$ . Antibodies were diluted in sterile saline and injected into the retro-orbital venous plexus.

Clinical isolate UL32-GFP-Merlin-WT and GFP-Merlin delta US2-11 strains were kind gifts from Dr. R. Stanton (University of Cardiff, UK). Primary antibodies used for the following proteins were obtained from commercial sources: IE1/2 (mouse monoclonal, 11–003, Argene), GFP (mouse monoclonal, 7.1 and 13.1, Roche), pp65 (mouse monoclonal, 1-L-11, Santa Cruz), anti-ubiquitin (mouse monoclonal, clone Ubi-1, Merck). The antibody for HA (12CA5) is a mouse hybridoma supernatant. Secondary HRP-conjugated goat anti-mouse/rabbit antibodies were from Jackson ImmunoResearch Laboratories. Purified anti-human leukocyte antigen (HLA-A,B,C) antibody (clone W6/32), and isotype control antibody (clone MOPC-173) were from Biolegend, anti-IFN-γ antibody (clone AF-285-NA) was from R&D systems. Antibodies against IFN-α (Clone MMHA-6) and IFN-β (Clone MMHB-3) were from PBL Assay Science and isotype control antibody (MOPC-21) was from Biolegend. Alexa Fluor 488-conjugated goat anti-mouse was from Invitrogen. ZVAD-fmk was from ENZO Life Sciences. Instant Blue stain was from Expedeon and MG132 was obtained from Sigma. Immunoblotted proteins were detected using Enhanced Chemiluminescence (ECL) [44 (link)] or ECL prime western blotting detection reagents (GE Healthcare) and ChemiDoc XRS+ (Bio-Rad).