Anti acetyl histone 3

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-acetyl-histone 3 is a laboratory product used for the detection and analysis of acetylated histone 3 proteins. It is a specific antibody that binds to the acetylated form of histone 3, allowing researchers to study epigenetic modifications and chromatin structure.

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Close spelling variants of this product

Histone 3 (79 citations) Anti-Histone 3 (29 citations) Acetyl-histone 3 (5 citations)

2 protocols using anti acetyl histone 3

Protein Lysates Western Blot Analysis

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Protein lysates were generated, and western [28 (link)] blots performed the following standard procedures [18 (link)]. All primary antibodies used in western blot analyses [anti-γH2AX, anti-phospho-CHK1 (Ser345), anti-phospho-CHK2 (Thr68), anti-phospho-BRCA1 (1524), anti-phospho-ATM (Ser 1981), anti-phospho-ATR (Ser 428), anti-p53, anti-acetyl-histone 3, anti-acetyl-histone 4, anti-Bcl-XL, anti-Mcl1, anti-Bcl2 and anti-AIF] were obtained from Cell Signaling, Boston, MA, USA. Horseradish peroxidase linked-donkey (anti-rabbit), sheep (anti-mouse), or mouse (anti-goat) immunoglobulins were used as secondary antibodies at a 1:5000 dilution (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

López-Iglesias A.A., Herrero A.B., Chesi M., San-Segundo L., González-Méndez L., Hernández-García S., Misiewicz-Krzeminska I., Quwaider D., Martín-Sánchez M., Primo D., Paíno T., Bergsagel P.L., Mehrling T., González-Díaz M., San-Miguel J.F., Mateos M.V., Gutiérrez N.C., Garayoa M, & Ocio E.M. (2017). Preclinical anti-myeloma activity of EDO-S101, a new bendamustine-derived molecule with added HDACi activity, through potent DNA damage induction and impairment of DNA repair. Journal of Hematology & Oncology, 10, 127.

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Western Blot Analysis of ER Stress Markers

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The heart samples in the sham and TAC group with or without 4-PBA were excised and homogenized. The following antibodies were used for western blotting: anti-GRP78 (sc-376768, Santa Cruz), anti-CHOP (#2895, Cell Signaling Technology), anti-β-actin (sc-47778, Santa Cruz), anti-phospho-PERK (sc-32577, Santa Cruz), anti-PERK (sc-13073, Santa Cruz), anti-Acetyl-Histone 3 (#9649, Cell Signaling Technology) and anti-Histone 3 (#4499, Cell Signaling Technology). Samples containing equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes. The membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated overnight at 4°C with the primary antibody. After being incubated with anti-mouse secondary antibody (#7076S, Cell Signaling Technology) or anti-rabbit secondary antibody (#7074S, Cell Signaling Technology) for 1 h at room temperature, the blots were detected in the dark room using autoradiography film (Denville Scientific, Inc) and quantified by densitometry using the Image J Analysis software (National Institutes of Health).

Luo T., Chen B, & Wang X. (2015). 4-PBA prevents pressure overload-induced myocardial hypertrophy and interstitial fibrosis by attenuating endoplasmic reticulum stress. Chemico-biological interactions, 242, 99-106.

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