Fe 3 chloride hexahydrate

The recombinant tissue-type plasminogen activator (rtPA, Actilyse^®) was purchased from Boehringer Ingelheim (Mannheim, Germany). Inactin^® (thiobutabarbital sodium), heparin, penicillin G, and stannous chloride dehydrate were purchased from Sigma (St. Louis, MO, USA). Fe (II) chloride tetrahydrate (≥99%), Fe (III) chloride hexahydrate (≥97%), and α-Naphthylisothiocyanate were purchased from Acros Organics (Morris Plains, NJ, USA). Isoflurane (FORAND^®) was purchased from Aesica Queenborough Limited (Queenborough, UK). The recombinant human plasminogen activator inhibitor-1 was purchased from Calbiochem (La Jolla, CA, USA). The chitosan polymer (150 kDa) with a degree of deacetylation of 95% was obtained from Fluka (Buchs, Switzerland). The chromogenic substrate, S-2288^TM, was obtained from Chromogenix (Milano, Italy).

The 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Dimethyldioctadecyl ammonium bromide (DDAB), cholesterol (CH), Triton X-100, and IR-780 iodide were acquired from Sigma-Aldrich (St. Louis, MO, USA). Fe(II) chloride tetrahydrate (99%) and Fe(III) chloride hexahydrate (97%) were purchased from Acros Organics (Geel, Belgium). Cell culture reagents were purchased from Life Technologies (Carlsbad, CA, USA). All chemicals and reagents were of analytical grade and were used as received.