EdU staining was performed according to the manufacturer's protocol of EdU Kit (Ribobio, Guangzhou, China). MCs seeded in six‐well plates at 2 × 105 cells/well were fixed with 3.7% paraformaldehyde and permeabilized with PBS‐Tween‐20 for 20 min, respectively, at 25°C. Then, each well was stained with 10 μM EdU and was cultured for 2 h. After 30 min of staining with DAPI (Sigma‐Aldrich, MO), the images were observed under a Leica DM200 microscope (Leica, Solms, Germany) and analyzed with ImageJ software (National Institutes of Health, MD).
Dm200 microscope
Manufactured by Leica
Sourced in Germany
The Leica DM200 is a compound microscope designed for routine microscopy applications. It features a robust construction, a wide range of magnification options, and a user-friendly interface. The DM200 enables the observation and analysis of a variety of biological and material samples.
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Lab products found in correlation
2 protocols using dm200 microscope
1
EdU Incorporation Assay in MCs
2
Histomorphometric Analysis of Hepatic Steatosis
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Paraffin sections were prepared and stained with hematoxylin and eosin to visualize general tissue morphology. Microscopic examination was done using a Leica DM 200 micro-scope (Leica Germany) and images were observed under polarized light at magnification 200x. The percentage steatosis and fat droplet size were assessed using a histomorphometric method. The fibrosis extent was measured as a percentage of picrosirius-red-positive area in the microscopic field. The evaluation was performed by an expert liver pathologist (PJ) who was blinded to the allocation of the samples.
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