The lyophilized W/O/W emulsions were removed from the vial and broken up into small mm sized pieces. A piece of aluminum was used as the imaging substrate. A thin layer of conductive silver paint (Agar Scientific Ltd, Essex, United Kingdom) was painted onto the aluminum substrate and the millimeter sized pieces were immediately placed on the paint. The samples were placed in an oven (Supplier) heated to 60 °C for 5 min to dry the paint and glue the sample to the substrate. An 8 nm layer of chrome was sputter-coated onto the sample to make it conductive using a JEOL JFC-2300HR high resolution fine sputter-coater (JEOL Ltd, Tokyo, Japan). The samples were imaged using JEOL JSM-7400F scanning electron microscope (JEOL Ltd).
Jsm 7400f scanning electron microscope
Manufactured by JEOL
Sourced in Japan
The JSM-7400F is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution imaging of samples by scanning them with a focused electron beam. The JSM-7400F is capable of producing detailed images of a wide range of materials, including metals, ceramics, polymers, and biological samples. The core function of this instrument is to enable users to examine the surface topography and composition of their samples at the micro- and nanoscale levels.
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Lab products found in correlation
Conductive silver paint, by Agar Scientific (1 mentions)
Ultracut e ultramicrotome, by Leica (1 mentions)
1230 transmission electron microscope, by JEOL (1 mentions)
Uv 3100pc, by Shimadzu (1 mentions)
Ags x, by Shimadzu (1 mentions)
Mueller hinton broth (mhb), by Thermo Fisher Scientific (1 mentions)
Gram positive staphylococcus aureus, by American Type Culture Collection (1 mentions)
Gram negative pseudomonas aeruginosa, by American Type Culture Collection (1 mentions)
Av 400 instrument, by Bruker (1 mentions)
Nanoscope analysis 2, by Bruker (1 mentions)
Escherichia coli, by American Type Culture Collection (1 mentions)
Candida albicans, by American Type Culture Collection (1 mentions)
Tryptic soy broth (tsb), by Thermo Fisher Scientific (1 mentions)
Em ace600, by Leica (1 mentions)
10 protocols using jsm 7400f scanning electron microscope
1
Preparation and Imaging of Lyophilized Emulsions
2
Ultrastructural Analysis of Tumor Spheroids
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Five spheroids from three tumors were collected, in each case, for transmission electron microscopy (TEM) or scanning electron microscopy (SEM) study. Spheroids were fixed for 3 hours in 2% glutaraldehyde in 0.1 M sucrose-adjusted cacodylate buffer (pH 7.4; 300 ± 10 mOsmol). Post fixation was performed for 1 hour in 1% OsO4 using the same buffer. The specimens were dehydrated with ethanol in concentrations increasing to 100%.
For TEM, embedding in Epon 812 was performed by using graded additions of Epon-propylenoxide mixtures. The final polymerization was carried out at 60°C for 3 days. Sections (60 nm thick) were cut with a diamond knife on a Reichert Ultracut E ultramicrotome (Leica, Buffallo, NY), collected on 200-mesh copper grids, and examined in a Jeol 1230 transmission electron microscope (Jeol, Tokyo, Japan) operated at 80 kV.
For SEM, the specimens were critical-point dried with CO2. They were then carefully mounted on stubs using tape and silver conductive paint. The specimens were coated with gold in a vacuum evaporator and examined with a Jeol JSM -7400 F Scanning Electron Microscope (Jeol, Tokyo, Japan).
For TEM, embedding in Epon 812 was performed by using graded additions of Epon-propylenoxide mixtures. The final polymerization was carried out at 60°C for 3 days. Sections (60 nm thick) were cut with a diamond knife on a Reichert Ultracut E ultramicrotome (Leica, Buffallo, NY), collected on 200-mesh copper grids, and examined in a Jeol 1230 transmission electron microscope (Jeol, Tokyo, Japan) operated at 80 kV.
For SEM, the specimens were critical-point dried with CO2. They were then carefully mounted on stubs using tape and silver conductive paint. The specimens were coated with gold in a vacuum evaporator and examined with a Jeol JSM -7400 F Scanning Electron Microscope (Jeol, Tokyo, Japan).
3
Glutaraldehyde Fixation and SEM Imaging of IVDs
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IVDs were fixed overnight using 2.5% (v/v) glutaraldehyde (EMS, Hatfield, PA, USA) in 0.1 M sodium cacodylate solution (EMS) at 4 °C, followed by 0.1 M sodium cacodylate incubation at 4 °C overnight. Then the IVDs were gently dehydrated in 50%, 60%, 70%, 80%, 90%, and 100% ethanol baths. Initial air-drying was followed by freeze-drying overnight before the IVDs were Au/Pd sputter coated and examined with a JEOL JSM-7400F scanning electron microscope (JEOL Ltd., Akishima, Tokyo, Japan) at 1500× magnification.
4
Multimodal Imaging of Eye and Brain
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Representative digital eye images were taken with a Leica 205C dissection microscope and Leica DSC290 HD camera system. In addition, samples were examined with a JEOL JSM 7400F scanning electron microscope (SEM). Brains were analyzed using confocal microscope and Z stack images of the brains were taken using bright field and 488nm filter.
5
Scanning Electron Microscopy of Biofilms
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The biofilm structure was assessed by scanning electron microscopy (SEM). Biofilm was cultured for 48 h as described above on 12 mm glass slides, then washed with PBS and fixed using 2% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, for 2 h at room temperature, and dehydrated in ascending concentrations of ethanol. Following critical point drying, slides were sputter coated with gold and mounted on stubs. Imaging was performed using Jeol JSM-7400F scanning electron microscope under magnification of 1500X.
6
SEM Imaging of Cypep-1 Treated 4T1 Cells
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4T1 mammary carcinoma cells growing on cover-glass slides were exposed to 35 µg/ml Cypep-1 for 60 minutes and for 6 hours, respectively. Thereafter, the slides were prepared for SEM as originally described by Anderson [75 ]. After mounting them on stubs with tape and silver paint, an approximately 50 nm thick gold layer was evaporated on the specimens, using a Polaron E5000 SEM Coating Unit (Polaron Components Ltd, Watford, UK). Finally, the specimens were examined with a Jeol JSM-7400F scanning electron microscope (Jeol Ltd, Tokyo, Japan).
7
Cu Grid Electrode Characterization and Capacitor Sensor Fabrication
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Scanning electron microscopy (SEM) images were obtained on a JSM-7400F scanning electron microscope (JEOL, Japan) at 10 kV. The samples were coated with a thin Pt layer before observation. The pattern heights were measured using a stylus surface profiler, Dektak XT-(ULVAC, Japan). Optical images of the surfaces were taken using a VW-9000/VW-600c digital microscope (KEYENCE, Japan). The optical transmittance of the Cu grid electrodes was measured using a UV-vis spectrometer, UV-3100PC (SHIMADZU, Japan); a baseline correction was performed using the bare PEN substrate, which was also used as a reference. For sheet resistance measurements, Au electrode pads with a gap distance of 5 mm were modified on the Cu grid electrodes by a simple sputtering deposition before the measurements. The sheet resistance of the Cu grid electrodes was measured by the four-probe method using a KB-100 (KB-esi, Germany). More than three measurements were performed for each sample, and the results were averaged. As for evaluation as a capacitor sensor, the sensor was fabricated by putting two grid electrodes together in a face to face manner as shown in the inset of Fig. 7 . Capacitance data of the sensor was collected by a precision LCR meter, 4284 A (Agilent Technologies, USA) when pressure was added by an autograph, AGS-X (Shimadzu Corporation, Japan).
8
Collagen Fibril Analysis in Tumor Tissues
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Tumor samples were taken from the tumor periphery and were fixed and processed as previously described [33 (link)]. A JEM-1230 Transmission Electron Microscope (TEM) (Jeol, Tokyo, Japan) was used to measure the diameter and organization of the collagen fibrils, and images from four to six different areas of the tissue were analyzed. Pictures were captured at × 100,000 magnification and analyzed using Image J 1.46 (National Institute of Health, Bethesda, MD., USA) to measure the fibril diameter. To investigate the organization of the collagen fibrils, pictures were captured at × 30,000 magnification and scored from one to four considering collagen fibril organization and alignment within the collagen fibers.
A JSM-7400F Scanning Electron microscope (Jeol) was used to study the tumor collagen fibril scaffold architecture. Five images from different areas of the tumor were captured from each tumor at × 10,000 magnification.
A JSM-7400F Scanning Electron microscope (Jeol) was used to study the tumor collagen fibril scaffold architecture. Five images from different areas of the tumor were captured from each tumor at × 10,000 magnification.
9
Antimicrobial Assessment of Styrene-Benzoyl Peroxide Materials
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All of the anhydrous solvents and chemicals were used as received from the commercial suppliers, unless otherwise indicated. Styrene (≥99%) (Lot: STBJ7184) and benzoyl peroxide (75%) (Lot: MKCG5941) were purchased from Aldrich (Poznan, Poland). 1H and 13C NMR spectra were recorded on Bruker (Billerica, MA, USA), AV-400 instrument (400 MHz). Chemical shifts (δ) were reported in parts per million (ppm). SEM images were obtained on a JEOL JSM-7400F scanning electron microscope (Akishima, Tokyo, Japan). AFM topographic images were obtained using a Nanoscope 9.7 Dimension ICON atomic force microscope (Bruker, Billerica, MA, USA) and the results were analysed using NanoScope Analysis 2.0 software (Bruker, Camarillo, CA, USA).
For antimicrobial assessments, tryptic soy broth (TSB) and Mueller Hinton broth (MHB) were purchased from Oxoid Ltd. (Basingstoke, UK), while yeast mold broth (YMB) was purchased from BD (Singapore). The broth solutions were prepared according to the manufacturer’s instructions. Gram-negative bacteria Escherichia coli (ATCC 8739), gram-positive Staphylococcus aureus (ATCC 6538), Gram-negative Pseudomonas aeruginosa (ATCC 9027), and fungi Candida albicans (ATCC 10231) were purchased from ATCC (Manassas, VA, USA) and re-cultured according to the suggested protocols.
For antimicrobial assessments, tryptic soy broth (TSB) and Mueller Hinton broth (MHB) were purchased from Oxoid Ltd. (Basingstoke, UK), while yeast mold broth (YMB) was purchased from BD (Singapore). The broth solutions were prepared according to the manufacturer’s instructions. Gram-negative bacteria Escherichia coli (ATCC 8739), gram-positive Staphylococcus aureus (ATCC 6538), Gram-negative Pseudomonas aeruginosa (ATCC 9027), and fungi Candida albicans (ATCC 10231) were purchased from ATCC (Manassas, VA, USA) and re-cultured according to the suggested protocols.
10
Bacterial Attachment on Poly-L-Lysine Coated Coverslips
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Glass coverslip discs were first washed in 100% EtOH for 1h and left to dry at RT under sterile conditions. The cleaned discs were immersed for 1h at RT in 0.01% poly-L-lysine solution and allowed to dry. Discs were placed one per well in a 24-well polystyrene cellrepellent plate and overlaid with 2 mL of overnight CYE bacterial cultures. Plates were then covered, sealed with Parafilm, and incubated overnight with shaking at 32 ºC. After incubation, the media was removed and the cells attached to the coverslips were fixed in 1 mL of 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) for at least 1 h and washed three times in 1 mL of 0.2 M cacodylate buffer for 5 min. After post-fixation in 500 µL of 1.33% osmium tetroxide (in 0.2 M cacodylate buffer) for 1 h, bacteria were dehydrated with increasing ethanol concentrations (25, 50, 75, 95 and 100%) . From the 100% EtOH bath, coverslips were criticalpoint-dried using CO2 (Leica EM ACE600), coated with 3 nm gold/palladium (Leica CPD300) and examined with a JEOL JSM-7400F scanning electron microscope (3 kV-LEI detector).
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