Ki 67 rm 9106 s1

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Ki-67 (RM-9106-S1) is a laboratory reagent used for the detection of the Ki-67 protein, which is a marker of cellular proliferation. This reagent is designed for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections.

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Lab products found in correlation

Ab64100, by Abcam (1 mentions) Ab5690, by Abcam (1 mentions) Mum 1, by Santa Cruz Biotechnology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) Ab5535, by Merck Group (1 mentions) Sox2 sc 17320, by Santa Cruz Biotechnology (1 mentions) Cdh1 610181, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti cd3, by Jackson ImmunoResearch (1 mentions) Facs antibodies, by Thermo Fisher Scientific (1 mentions) Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions)

4 protocols using ki 67 rm 9106 s1

Immunohistochemical Analysis of Murine Tissues

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Tissues were fixed in 4% Paraformaldehyde overnight and embedded in paraffin according to standard procedures. 5μm sections were either stained with Hematoxilin&Eosin or with the following antibodies: CD45R/B220 (ab64100, Abcam), CD3 (ab5690, Abcam), Ki-67 (RM-9106-S1, Thermo Scientific), IgG (BA2000, Vector), BRAF (sc-9002, Santa Cruz), pERK (4373, Cell Signaling), Bcl-6 (5650, Cell Signaling), and Mum1 (sc-6059, Santa Cruz). Organs from at least 5 mice from different litters were used for all stainings.

Karreth F.A., Reschke M., Ruocco A., Ng C., Chapuy B., Léopold V., Sjoberg M., Keane T.M., Verma A., Ala U., Tay Y., Wu D., Seitzer N., Del Castillo Velasco-Herrera M., Bothmer A., Fung J., Langellotto F., Rodig S.J., Elemento O., Shipp M.A., Adams D.J., Chiarle R, & Pandolfi P.P. (2015). The BRAF pseudogene functions as a competitive endogenous RNA and induces lymphoma in vivo. Cell, 161(2), 319-332.

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Immunohistochemistry of Fetal Lung Tissue

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Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human fetal lungs (T21 and non-T21) as previously described [27 (link)]. In brief, paraffin-embedded lungs were sectioned at 5 μm, rehydrated, and then boiled in a sodium citrate antigen retrieval solution for 12 min. A 15-min step in 3% H₂O₂ was performed to quench endogenous peroxidases to decrease background. Sections were incubated with the following primary antibodies overnight at 4 °C: CD31 (RB-10333-P0, 1:200; Neomarkers, Fremont, CA, USA), CDH1 (610181, 1:200; BD Biosciences, San José, CA, USA), Ki67 (RM-9106-S1, 1:200; Thermo Fisher Scientific, Fremont, CA, USA), LYVE1 (ab14917, 1:100; Abcam, Cambridge, UK), SOX2 (sc-17320, 1:100; Santa Cruz, Dallas, TX, USA), and SOX9 (AB5535, 1:500; Millipore, Burlington, MA, USA). Sections were then stained with appropriate fluorochrome-conjugated secondary antibodies in conjunction with Cy3-ACTA2 conjugated antibody (C6198, 1:200; Sigma-Aldrich, St Louis, MO, USA) when necessary. Slides were counterstained with DAPI (DE571; Life Technologies, Grand Island, NY, USA) and mounted using ProLong Diamond Antifade Mountant (Life Technologies).

Danopoulos S., Bhattacharya S., Deutsch G., Nih L.R., Slaunwhite C., Mariani T.J, & Al Alam D. (2021). Prenatal histological, cellular, and molecular anomalies in trisomy 21 lung. The Journal of pathology, 255(1), 41-51.

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Antibody-Based Tumor and Lineage Analysis

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Antibodies used for tumor analysis include F4/80 (ab6640, Abcam, Cambridge, MA, USA) [99 (link)], EphA2 (C-20, SC-924, Santa Cruz Biotech, Dallas, TX, USA), Ki-67 (RM-9106-S1, Thermo Fisher Scientific, Waltham, MA, USA), SREBP1 (H-160), SREBP2 (H-164), FASN (H-300), and CD31 (CM303A, Biocare Medical, Concord, CA, USA). Antibodies used for lineage analysis were: anti-B220 (RA3-6B2), anti-CD19 (eBio1D3), anti-IgM µ-chain (Jackson Labs), anti-CD43 (S7) for B cells, anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8a (53-6.7) for T cells, anti-CD41 (MWReg30), anti-CD71 (C2), anti-Ter119 (TER-119), and anti-F4/80 (BM8), anti-Mac1 (M1/70), and anti-Gr1 (RB6-8C5) for myeloid cells. Antibodies used for HSC and progenitor analysis were: Lineage (biotin-conjugated anti-Gr-1 (RB6-8C5), -Mac1 (M1/70), -B220 (RA3-6B2), -CD19 (eBio1D3), -Ter110 (TER-119), -CD5 (53-7.3), -CD4 (GK1.5), -CD8 (53-6.7)), APC-Cy7-c-Kit (2B8), PerCP-cy5.5-Sca1 (E13-161.7 or D7, 1:1000 dilution), FITC-CD48 (HM48-1), PE-Cy7-CD150 (TC15-12F12.2), APC-CD34 (RAM34) and PE–Flk2 (A2F10.1). All antibodies were used at 1:200 dilution unless otherwise noted. FACS antibodies were purchased from eBioscience (San Diego, CA, USA), BD Biosciences (San Jose, CA, USA) or BioLegend (San Diego, CA, USA).

Jiao X., Tian L., Zhang Z., Balcerek J., Kossenkov A.V., Casimiro M.C., Wang C., Liu Y., Ertel A., Soccio R.E., Chen E.R., Liu Q., Ashton A.W., Tong W, & Pestell R.G. (2021). Pparγ1 Facilitates ErbB2-Mammary Adenocarcinoma in Mice. Cancers, 13(9), 2171.

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Immunohistochemical Analysis of Prostate Cancer

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Immunohistochemical analysis of human prostate cancer was conducted using a polyclonal CAMK2N1 antibody ⁸. Human prostate cancer tissue arrays were purchased from Biomax. FOUR-micrometer sections were prepared from paraffin-embedded C4-2 Tumor tissues derived from nude mice, and tissues were extracted from paraffin. Tumor tissues were stained with primary antibody including Ki67 (RM-9106-S1, Thermo), Bcl-2 (SC-7382, Santa cruz), Bax (SC-7480, Santa cruz), p21 (SC-6246, Santa cruz).

Wang T., Guo S., Liu Z., Wu L., Li M., Yang J., Chen R., Liu X., Xu H., Cai S., Chen H., Li W., Xu S., Wang L., Hu Z., Zhuang Q., Wang L., Wu K., Liu J., Ye Z., Ji J.Y., Wang C, & Chen K. (2014). CAMK2N1 inhibits prostate cancer progression through androgen receptor-dependent signaling. Oncotarget, 5(21), 10293-10306.

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