Cd127 clone a7r34

Manufactured by BioLegend

CD127 (clone A7R34) is a mouse monoclonal antibody that recognizes the interleukin-7 receptor alpha (IL-7Rα) chain, also known as CD127. CD127 is a type I transmembrane protein and a key component of the receptor for the cytokine interleukin-7 (IL-7), which plays an important role in the development and homeostasis of T cells.

Automatically generated - may contain errors

Lab products found in correlation

Ifn γ clone xmg1, by Thermo Fisher Scientific (2 mentions) Cd45 clone 30 f11, by BioLegend (2 mentions) Brefeldin a golgiplug, by BD (1 mentions) Cytofix perm kit, by BD (1 mentions) Cd45.1 clone a20, by BD (1 mentions) Cd4 clone rm4 5, by BD (1 mentions) Cd11c clone hl3, by BD (1 mentions) β mercaptoethanol, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Cd45.2 clone 104, by BioLegend (1 mentions) Cd69 clone h1.2f3, by BioLegend (1 mentions) Cd62l clone mel 14, by BD (1 mentions) Mhcii clone m5 114, by BioLegend (1 mentions) Cd3 clone 17a2, by BioLegend (1 mentions) Cd8 clone 53 6, by BioLegend (1 mentions) Collagen 4, by Abcam (1 mentions) Cd8α clone 53 6, by Thermo Fisher Scientific (1 mentions) Cd44 clone im7, by BD (1 mentions) Klrg1 clone 2f1, by BD (1 mentions) Tnf α clone tn3 19, by BD (1 mentions)

5 protocols using cd127 clone a7r34

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell cultures were performed in complete culture medium consisting of RPMI-1640 supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine (Sigma, St. Louis, MO), 50 μg/ml gentamicin (PAA, Linz, Austria), and 50 μM beta-mercaptoethanol (Sigma).
Phenotypical analyses were performed by flow cytometry with mAb against CD4 (clone RM4-5), CD45.1 (clone A20), MHC class II (anti-I-A/I-E^diverse, clone 2G9), CD11c (clone HL3), CD8α (clone Ly-2), CD45 (clone 30-F11), CD103 (clone M290), EpCAM/CD326 (clone G8.8), CD19 (clone 1D3), NK1.1 (clone PK136), IL-12p40 (clone C15.6) (all from BD-Pharmingen, San Diego, CA), CD127 (clone A7R34, Biolegend, San Diego, CA), CD70 (clone FR70, Biolegend), and Langerin/CD207 mAb (clone 929F3; Dendritics, Lyon, France). When possible, viable cells were determined by exclusion of 7-AAD-positive dead cells (BD-Pharmingen). IL-12p40 and IL-10 stainings were performed on total lymph node cell suspension (10⁶ cells/ml) incubated for 3 h in 1 μg/ml Brefeldin A (Golgiplug, BD-Pharmingen). To stain for Langerin or intracellular cytokines, permeabilization was performed with Cytofix/perm kit (BD-Pharmingen).

Flacher V., Tripp C.H., Mairhofer D.G., Steinman R.M., Stoitzner P., Idoyaga J, & Romani N. (2014). Murine Langerin+ dermal dendritic cells prime CD8+ T cells while Langerhans cells induce cross-tolerance. EMBO Molecular Medicine, 6(9), 1191-1204.

+ Open protocol

+ Expand

Comprehensive Immune Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for staining sections for imaging or isolated cells for flow cytometry: collagen-IV (rabbit polyclonal; Abcam), gp38 (clone 8.1.1; eBioscience), PNAd (clone Meca-79; BD Biosciences), ER-TR7 (clone sc-73355; Santa Cruz Biotechnology), CD11c (clone N418 or HL3; BioLegend), Lyve1 (clone Aly7; eBioscience), SIRPa (clone P84; BD Biosciences), CD11b (clone 5C6; AbD Serotec; clone M1/70; BioLegend), Y-Ae (clone eBioY-Ae; eBioscience), CD45.2 (clone 104; BioLegend), MHC II (clone M5/114.15.2; BioLegend), CD205 (clone NLDC-145; BioLegend), CD207 (clone 929F3.01; Dendritics), CD3 (clone 17A2; BioLegend), CD69 (clone H1.2F3; BioLegend), CD62L (clone Mel-14; BD Biosciences), CD8 (clone 53-6.7; BioLegend), IFNγ (clone XMG1.2; eBiosciences), CD127 (clone A7R34; BioLegend), and KLRG1 (clone 2F1; eBiosciences).

Gerner M.Y., Casey K.A., Kastenmuller W, & Germain R.N. (2017). Dendritic cell and antigen dispersal landscapes regulate T cell immunity. The Journal of Experimental Medicine, 214(10), 3105-3122.

+ Open protocol

+ Expand

Phenotypic Characterization of CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Red blood cell (RBC)-lysed blood and spleen samples were stained for 30 min at 4°C with mAb against the following molecules: CD44 (clone IM7, BD), CD127 (clone A7R34, Biolegend), KLRG1 (clone 2F1, BD), and CD8α (clone 53-6.7, eBioscience). H-2D^b LT359 tetramers were provided by the NIH Tetramer Core Facility (Atlanta, GA). Samples were acquired on a LSRII instrument (BD Biosciences) and data analyzed using FlowJo software (TreeStar, Inc., Ashland, OR). For intracellular cytokine staining (ICS), cells were stimulated for 5 h with LT359-368 peptide (1 µM) in the presence of Golgiplug (BD Biosciences). Cells were surface stained, permeabilized and fixed using the Cytofix/Cytoperm kit (BD Biosciences), and stained with mAbs specific for IFN-γ (clone XMG1.2, eBioscience) and TNF-α (clone TN3-19.12, BD Biosciences). Nucleated cells from blood and spleen samples were counted with TC20™ automated cell counter (Bio-Rad). The number of LT359 tetramer⁺ CD8 T cells was calculated as the total number of nucleated cells × the percentage of specific subpopulation out of total population as determined by using FlowJo software.

Qin Q., Lauver M., Maru S., Lin E, & Lukacher A.E. (2017). Reducing persistent polyomavirus infection increases functionality of virus-specific memory CD8 T cells. Virology, 502, 198-205.

+ Open protocol

+ Expand

Isolation and Culture of CHILPs

Check if the same lab product or an alternative is used in the 5 most similar protocols

OP9 stromal cells were cultured and irradiated in a cell culture plate in advance. CHILPs were isolated and purified from bone marrow of the donor mice, gated in 7AAD^–lineage^–CD127⁺α₄β₇⁺CD25^–Flt3^–, stained with the following antibodies: 7AAD (BioLegend, 420404), CD127 (clone A7R34, BioLegend, 135023), α₄β₇ (clone DATK32, BioLegend, 120608), CD25 (PC61, BioLegend, 102006), Flt3 (clone A2F10, BioLegend, 135312), CD45 (clone 30-F11, BioLegend, 103128) and lineage cocktail as mentioned before. CHILPs were cultured on OP9 cells with 20 ng/ml recombinant mouse IL-7 (Novoprotein, CC73) and 10 ng/ml recombinant mouse SCF (Novoprotein, C775) in Alpha-MEM medium including 15% FBS, 10 mM HEPES, 100 U/ml Penicillin, 100 μg/ml Streptomycin, 1 mM sodium pyruvate, 8 mg/ml Glutamine and 80 μM 2-Mercaptoethanol. 5 μM 4-hydroxytamoxifen (MCE, HY-16950) and other drugs were added to cell culture medium in an optimal dose to treat CHILPs in vitro. Cell sorting was performed on the FACSAria (BD Bioscience).

Hao J., Liu C., Gu Z., Yang X., Lan X, & Guo X. (2024). Dysregulation of Wnt/β-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells. Nature Communications, 15, 2820.

+ Open protocol

+ Expand

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibiotics vancomycin, polymyxin B, ampicillin, and neomycin were obtained from Gold Biotechnology, Inc. (St. Louis, MO), and metronidazole was from Sigma-Aldrich Corp. (St. Louis, MO). Liposomal clodronate and control liposomes were obtained from Encapsula NanoSciences LLC (Brentwood, TN). The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet^TM fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11, BioLegend), CD11b (clone M1/70, BioLegend), CD11c (clone N418, BioLegend), CD103 (clone 2E7, eBioscience/ Thermo Fisher Scientific, Waltham, MA), CD64 (clone X54-5/7.1, BioLegend), I-A/I-E (MHCII, clone M5/114.15.2, BioLegend), Ly6C (clone HK1.4, BioLegend), CD3e (clone 145–2 C11, BioLegend), CD4 (clone GK1.5, BioLegend), FoxP3 (clone FJK-16s, eBioscience), IFNγ (clone XMG1.2, BioLegend), IL17A (clone TC11-18H10.1, BioLegend), CD127 (clone A7R34, BioLegend), CD90.2 (Thy1.2, clone 30-H12, BioLegend), and RORγt (clone B2D, eBioscience).

Redhu N.S., Bakthavatchalu V., Conaway E.A., Shouval D.S., Tsou A., Goettel J.A., Biswas A., Wang C., Field M., Muller W., Bleich A., Li N., Gerber G.K., Bry L., Fox J.G., Snapper S.B, & Horwitz B.H. (2017). Macrophage dysfunction initiates colitis during weaning of infant mice lacking the interleukin-10 receptor. eLife, 6, e27652.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!