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Fitc rat anti mouse ly 6g mab clone 1a8

Manufactured by BD

The FITC rat anti-mouse Ly-6G mAb (Clone 1A8) is a flow cytometry antibody that binds to the Ly-6G antigen expressed on murine neutrophils. It is conjugated with the fluorescent dye FITC for detection purposes.

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2 protocols using fitc rat anti mouse ly 6g mab clone 1a8

1

Fluorescent Antibody and Dye Labeling

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Fluorescein-isothiocyanate (FITC) rat anti-mouse Ter-119 mAb (Clone Ter-119) and FITC rat anti-mouse Ly-6G mAb (Clone 1A8) were purchased from BD Biosciences (San Jose, CA). Rat anti-mouse Gr-1 mAb (clone RB6–8C5) was prepared from a hybridoma culture supernatant at the Lymphocyte Culture Center, University of Virginia. The Gr-1 mAb was conjugated to Alexa Fluor 546 using an antibody labeling kit from Molecular Probes, Inc. (Eugene, OR). Texas Red dextran (MW 70,000) and FITC dextran (MW 70,000) were also purchased from Molecular Probes Inc. Evans blue was purchased from Sigma Aldrich (Saint Louis, MO).
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2

Multicolor Staining of Mouse Immune Cells

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Fluorescein-isothiocyanate (FITC) rat anti-mouse Ter-119 mAb (Clone Ter-119) and FITC rat anti-mouse Ly-6G mAb (Clone 1A8) were purchased from BD Biosciences (San Jose, CA). Rat anti-mouse Gr-1 mAb (clone RB6-8C5) was prepared from a hybridoma culture supernatant at the Lymphocyte Culture Center, University of Virginia. The Gr-1 mAb was conjugated to Alexa Fluor 546 using an antibody labeling kit from Molecular Probes, Inc. (Eugene, OR). Texas Red dextran (MW 70,000) and FITC dextran (MW 70,000) were also purchased from Molecular Probes Inc. Evans blue was purchased from Sigma Aldrich (Saint Louis, MO).
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