Antigen retrieval was performed on paraffin-embedded allograft adipose matrix with 10 mM sodium citrate buffer. Primary collagen I, III, IV, and VI antibodies (Abcam, Cambridge, Mass.) were used with hematoxylin counterstain (Sigma-Aldrich, St. Louis, Mo.). Growth factors were extracted using 4 M guanidine hydrochloride in 0.05 M Trizma (Sigma-Aldrich) or T-PER (Thermo Fisher, Waltham, Mass.) over 48 hours at 4°C. Growth factors were quantified using Luminex multiplexing (MilliporeSigma, St. Louis, Mo.) or by enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, Minn.).
Hematoxylin counterstain
Manufactured by Merck Group
Hematoxylin counterstain is a laboratory reagent used in histology and cytology to provide a contrasting stain for cell nuclei. It is a basic dye that binds to acidic components within the cell, primarily the DNA in the nucleus. This staining technique allows for the visualization and differentiation of cellular structures during microscopic examination.
Automatically generated - may contain errors
Lab products found in correlation
Enzyme linked immunosorbent assay, by R&D Systems (1 mentions)
T per, by Thermo Fisher Scientific (1 mentions)
Trizma, by Merck Group (1 mentions)
Interleukin 6 (il 6), by Novus Biologicals (1 mentions)
Cd163, by Novus Biologicals (1 mentions)
Novared chromagen, by Vector Laboratories (1 mentions)
Il 15, by R&D Systems (1 mentions)
2 protocols using hematoxylin counterstain
1
Allograft Adipose Matrix Characterization
2
Immunohistochemical Analysis of Macaque Tissues
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Macaque tissue was fixed in 10% formalin and paraffin embedded. Five μm sections were stained with hematoxylin and eosin and examined microscopically. Indirect immunohistochemical staining for IL15 (R&D Systems), NOS2 (Cayman Chemical, Ann Arbor, MI, USA), IL6 and CD163 (both Novus Biologicals, Litleton, CO, USA), were performed as previously described (Roberts et al., 2003 (link); Marcondes et al., 2007 (link)). Colorimetric development was performed with the NovaRed chromagen (Vector) followed by a hematoxylin counterstain (Sigma-Aldrich).
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