Facs calibur hg flow cytometer

Manufactured by BD

Sourced in United Kingdom

The FACS Calibur HG flow cytometer is a laboratory instrument designed to analyze and sort cells and particles in a fluid stream. It utilizes laser technology to measure and detect the physical and chemical characteristics of cells or particles as they flow through a detection chamber.

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Lab products found in correlation

Cellquest software, by BD (3 mentions) Collagenase 1, by Fujifilm (2 mentions) Dnase 1, by Fujifilm (2 mentions) Clone fjk 16s, by Abcam (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Cellquest pro software, by BD (2 mentions) I a 1 e, by BD (1 mentions) Anti cd16 32 2.4g2, by BD (1 mentions) Cd11b, by BioLegend (1 mentions) F4 80, by BioLegend (1 mentions) Rnase a, by Merck Group (1 mentions) Clone 53 6, by BioLegend (1 mentions) Epr3915, by Abcam (1 mentions) Clone gk1, by BioLegend (1 mentions)

Close spelling variants of this product

Calibur flow cytometer (187 citations) FACS flow cytometer (328 citations) Calibur cytometer (14 citations) Calibur (250 citations) BD FACSTM (2 citations)

6 protocols using facs calibur hg flow cytometer

Phenotypic Analysis of Tumor Immune Cells

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Tumors were harvested and processed using Collagenase I and DNase I (Wako, Osaka, Japan). The resulting cell suspensions were clarified using 40-μm filters to prepare single cell suspensions, and single cells were suspended in PBS supplemented with 2% FBS. Splenocytes were hemolyzed and incubated with anti-CD16/32 2.4G2 (BD Biosciences, San Jose, CA, USA) to reduce FcγR binding. Cell-surface antigens were stained with antibodies specific for CD8 (BioLegend, San Diego, CA, USA, clone 53-6.7), CD4 (BioLegend, clone GK1.5), CD45 (BioLegend, clone 30-F11), IA/IE (BD Bioscience, clone M5/114), CD11c (BD Bioscience, clone HL3), CD11b (BioLegend, clone M1/70), and F4/80 (BioLegend, clone BM8).
For intracellular staining, cells were fixed and permeabilized using Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA, USA) after cell surface staining and were stained with labeled antibodies against the intracellular molecules foxp3 (eBioscience, clone FJK-16s), glucose transporter 1 (GLUT1, Abcam, Cambridge, UK, clone EPR3915), and hexokinase II (HX2, Abcam, clone EPR20839).
Samples were analyzed on a FACS Calibur HG flow cytometer (BD Biosciences). Data analysis was performed with CellQuest™ software (Becton Dickinson, Lincoln Park, NJ, USA).

Tomita M., Yasui H., Higashikawa K., Nakajima K., Takakura H., Shiga T., Kuge Y, & Ogawa M. (2018). Anti PD-1 treatment increases [18F]FDG uptake by cancer cells in a mouse B16F10 melanoma model. EJNMMI Research, 8, 82.

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Apoptosis Assay in PC9 Cells

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Cell apoptosis were employed by flow cytometry. PC9 cells were seeded in 6-well plates at a density of 3×10⁵ cells/well, and treated with either 0.1 μM icotinib, 200 μg/mL thalidomide, or a combination. After 24-hour culture, cells were collected and stained with Annexin V-FITC and propidium iodide (Apoptosis Kit, BestBio Biotechnology). Data were acquired on a FACSCalibur HG flow cytometer (BD, Franklin Lakes, NJ, USA) and analyzed using Cell-Quest software (BD Bioscience). For each experiment, 10 000 events per sample were recorded. FlowJo 7.6 software was used for flow cytometry analysis. All experiments were repeated 3 times.

Sun X., Xu Y., Wang Y., Chen Q., Liu L, & Bao Y. (2018). Synergistic Inhibition of Thalidomide and Icotinib on Human Non-Small Cell Lung Carcinomas Through ERK and AKT Signaling. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 3193-3203.

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Cell Cycle Analysis by Flow Cytometry

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OCCC cell lines were plated at a density 1×10⁵cells/well in 6-well plates and cultured for 24 h. The cells were then transfected with siSMYD2 or siNC. Following 48–72 h incubation, the cells were digested with trypsin, washed with PBS and then added to ice-cold 70% ethanol for 2 h at 4°C. The samples were then incubated overnight at 4°C, washed with PBS, then treated with 0.25 mg/ml RNase A (Sigma-Aldrich; Merck KGaA) at 37°C for 30 min. Propidium iodide (final concentration, 50 µg/ml) was then added to each sample and incubated for 30 min in the dark at 4°C. Cell cycle progression was then evaluated using a FACSCalibur HG flow cytometer (BD Biosciences). The data were analyzed using the CellQuest Pro software (version 3.1; BD Biosciences).

Kojima M., Sone K., Oda K., Hamamoto R., Kaneko S., Oki S., Kukita A., Kawata A., Honjoh H., Kawata Y., Kashiyama T., Sato M., Taguchi A., Miyamoto Y., Tanikawa M., Tsuruga T., Nagasaka K., Wada-Hiraike O., Osuga Y, & Fujii T. (2020). The histone methyltransferase SMYD2 is a novel therapeutic target for the induction of apoptosis in ovarian clear cell carcinoma cells. Oncology Letters, 20(5), 153.

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Immune Profiling of Tumor Microenvironment

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Thirteen mice were used for flow-cytometry (cGAMP alone group n = 6, cGAMP/anti-PD-1 combination group n = 7). Tumors were harvested and processed using Collagenase I and DNase I (Wako, Osaka, Japan). The resulting cell suspensions were clarified using 40-μm filters to prepare single cell suspensions, and single cells were suspended in PBS supplemented with 2% FBS. Splenocytes were hemolyzed and incubated with anti-CD16/32 2.4G2 antibody (BD Biosciences, San Jose, CA) to reduce FcγR binding. Cell-surface antigens were stained with antibodies specific for CD8 (BioLegend, San Diego, CA, clone 53-6.7), CD4 (BioLegend, clone GK1.5), and CD45 (BioLegend, clone 30-F11).
For intracellular staining, cells were fixed and permeabilized using a Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA) after cell surface staining and then stained with labeled antibodies against the intracellular molecules Foxp3 (eBioscience, clone FJK-16s), glucose transporter 1 (GLUT1, Abcam, Cambridge, UK, clone EPR3915), and hexokinase II (HX2, Abcam, clone EPR20839).
Samples were analyzed on a FACS Calibur HG flow cytometer (BD Biosciences). Data analysis was performed with CellQuest™ software (Becton Dickinson, Lincoln Park, NJ).

Tomita M., Suzuki M., Kono Y., Nakajima K., Matsuda T., Kuge Y, & Ogawa M. (2020). Influence on [18F]FDG uptake by cancer cells after anti-PD-1 therapy in an enforced-immune activated mouse tumor. EJNMMI Research, 10, 24.

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Caspase Detection in Apoptosis

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Detection of active caspases was conducted using an Apoptosis Detection Kit Caspase Assay (Immunochemistry Technologies, Bloomington, MN). Briefly, trypsinised cells were labelled with SR-VAD-FMK for 1 h at 37°C, washed in 1× apoptosis wash buffer and placed on ice in the dark. SR-VAD-FMK is labeled with sulforhodamine B, a red fluorescent dye with optimal excitation at 565 nm and emission at 590–600 nm. Caspase activity was detected using a FACSCalibur HG flow cytometer (Becton Dickinson, Franklin Lakes, NJ) and an emission filter associated with the FL-2 channel.

Horii T., Yamamoto M., Morita S., Kimura M., Nagao Y, & Hatada I. (2015). p53 Suppresses Tetraploid Development in Mice. Scientific Reports, 5, 8907.

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Flow Cytometry Analysis of Modified Jurkat Cells

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APC-conjugated anti-human CD132 (clone: TUGh4) and APC-Rat IgG2b, k Isotype ctrl (clone: RTK4530) (BioLegend, San Diego, CA) antibodies were used for flow cytometry analyses of genome-modified Jurkat cells. Flow cytometry was performed on a FACSCalibur HG flow cytometer with CELL Quest Pro software (Becton Dickinson, Franklin Lakes, NJ). Dead cells were excluded by gating of forward and side scatter.

Matsubara Y., Chiba T., Kashimada K., Morio T., Takada S., Mizutani S, & Asahara H. (2014). Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. Scientific Reports, 4, 5043.

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