Log In Sign up
The largest database of trusted experimental protocols

Hiscript q rt supermix with gdna eraser

Manufactured by Vazyme
Visit Supplier
Sourced in China

HiScript Q RT Supermix with gDNA Eraser is a reverse transcription reagent designed for the synthesis of first-strand cDNA from total RNA. It includes a gDNA Eraser component for effective removal of genomic DNA contamination.

Automatically generated - may contain errors

Lab products found in correlation

Cfx96 rt pcr system, by Bio-Rad (1 mentions) Sybr green qpcr mix, by Vazyme (1 mentions) Trizol, by Vazyme (1 mentions) Chamq sybr qpcr master mix, by Vazyme (1 mentions) Abi 7500, by Thermo Fisher Scientific (1 mentions) Rna isolator, by Vazyme (1 mentions)

Spelling variants of this product

HiScript Q RT SuperMix (164 citations) GDNA Eraser (7 citations)

2 protocols using hiscript q rt supermix with gdna eraser

1

RNA Extraction and qPCR Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total RNA was isolated from cells using TRIzol (Vazyme, Nanjing, China). Total RNA was reversely transcripted into cDNA using hiScript Q RT Supermix with gDNA Eraser (Vazyme). SYBR Green qPCR mix (Vazyme) was used to perform qPCR in the CFX96 RT-PCR System (Bio-Rad, USA). The mRNA relative expression was calculated using 2−ΔΔCt method. Primer sequences were listed in the supplementary file (Additional file 1: Table S1).
Li Y., Gao Y., Jiang X., Cheng Y., Zhang J., Xu L., Liu X., Huang Z., Xie C, & Gong Y. (2022). SAMHD1 silencing cooperates with radiotherapy to enhance anti-tumor immunity through IFI16-STING pathway in lung adenocarcinoma. Journal of Translational Medicine, 20, 628.
+ Open protocol
+ Expand
2

Real-Time qPCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using RNA isolator (R401-01, Vazyme, Nanjing, China) and reverse-transcribed into cDNA using a HiScript qRT SuperMix with gDNA Eraser (R122-01, Vazyme) according to the manufacturer’s instructions. RT-qPCR was performed on an ABI 7500 (Applied Biosystems, Shanghai, China) using the ChamQ SYBR qPCR Master Mix (Q311-02, Vazyme, Nanjing, China). Primers for RT-qPCR are listed in Supplemental Table S1. Gene expression levels were calculated using the 2−ΔΔCt method and normalized to β-actin mRNA expression.
Guo R., Chen F, & Shi Z. (2020). Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells. Genes, 11(2), 120.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!