1600 ftir spectrophotometer

All reagents and solvents were purchased from Sigma-Aldrich (Darmstadt, Germany) and used without further purification. Melting points were measured in open capillary tubes with a Buchi 530 melting point apparatus and are uncorrected. IR (KBr) spectra were recorded using a Perkin-Elmer 1600 FTIR spectrophotometer. ¹H-NMR, HSQC, and ¹³ (link)C-NMR (proton decoupled) spectra were recorded on a Varian^UNITYINOVA 500 MHz spectrometer and Agilent VNMRS 600 MHz NMR spectrometer. Elemental analyses were performed on a Carlo Erba Model 1106 elemental analyser. Mass spectra (LC/MS-APCI) were recorded on a Finnigan^TM LCQ^TM Mass Spectrometer in the negative ionisation mode.

The FT-IR spectra of DAS, HP-β-CD, and DAS/HP-β-CD solid complex were recorded with a Perkin-Elmer 1600 FTIR spectrophotometer dispersing each sample in KBr for spectroscopy (2 mg of sample in 200 mg of KBr) [32 (link)]. The scan range used was 400–4000 cm⁻¹, with a resolution of 1 cm⁻¹. The instrument was periodically calibrated.

All reagents and solvents were of reagent grade quality and were obtained from commercial suppliers. All solvents were dried and purified as described by Perrin and Armarego.²⁶ Sulphanilamide, Sepharose 4B, protein assay reagents, 4-nitrophenylacetate were obtained from Sigma-Aldrich Co. All other chemicals were analytical grade and obtained from Merck.
The IR spectra were recorded on a Perkin Elmer 1600 FT-IR spectrophotometer, using KBr pellets. ¹H and ¹³C-NMR spectra were recorded on a Bruker Avance III 400 MHz spectrometers in CDCl₃ and chemical shifts were reported (δ) relative to Me₄Si as internal standard. MALDI-MS of complexes were obtained in dihydroxybenzoic acid as the MALDI matrix, using a nitrogen laser accumulating 50 laser shots, with a Bruker Microflex LT MALDI-TOF mass spectrometer. Optical spectra in the UV-Vis region were recorded with a Perkin Elmer Lambda 25 spectrophotometer.

The functional groups of Ch-MO NPs was analyzed by FT-IR spectroscopy with KBr discs (5 mg of Ch-MO NPs and 100 mg KBr pellets), in the range from 4000 to 400 cm⁻¹, with a resolution of 4.0 cm⁻¹ on a Perkin Elmer 1600 FT-IR Spectrophotometer (USA) [20 (link)].

The morphologies of the products were analyzed with a JSM-7900 scanning electron microscope (JEOL, Tokyo, Japan). The samples for SEM were mounted on aluminium studs using adhesive graphite tape and sputter-coated with gold before analysis. TEM characterization was studied on a JEOL JSM-2100 instrument. The samples for TEM images were prepared by sonicating the samples in chloroform until equally distributed then the samples were drop casted on a copper grid covered with carbon. Infrared spectra were measured in the range 400–4000 cm⁻¹ on PANI pellets made with KBr at a 1600 FTIR spectrophotometer (Perkin Elmer, Shelton, CT, USA) taking 10 scans at a resolution of 4 cm⁻¹. Raman spectra were recorded using a RM 1000 laser Raman (He–Ne ion, Renishaw, West Dundee, IL, USA) containing a metallurgical microscope (Olympus Valley, PA, USA) and a CCD detector. The laser power at the sample was kept below ~0.74 mW to avoid thermal degradation.

Melting points are reported uncorrected. Infrared (IR) spectra were obtained by a Perkin Elmer 1600 FTIR spectrophotometer with KBr. Proton and carbon nuclear magnetic resonance (¹H & ¹³C-NMR) spectra were recorded using a Varian VXR-300s instrument with Me4Si and CDCI₃ as the internal standard. Chemical shifts are reported in parts per million (ppm), and the abbreviations are used to describe the signals observed (s, singlet; br, broad; m, multiplet centered at; d, doublet; t, triplet). When deuterium (D₂O) was added, the O-H signals disappeared without significant alterations in other regions of the NMR spectra. Ultraviolet (UV) spectra were captured in chloroform using a Perkin Elmer 1800 FTIR instrument. To visualize spots and track the advancement of reactions, silica gel G-coated TLC plates were employed, along with iodine. Chromatograms were developed using mixtures of light petroleum-benzene and CHC1₃-acetone. Acetone was used to crystallize the product for further purification, and anhydrous sodium sulfate was employed as a desiccant to dry a solution.