C57bl 6 and balb c mice

C57BL/6 and BALB/c mice were purchased from Koatech (Gyeonggi-do, Korea), and mSirt1^−/− mice were kindly provided by Prof. Byung-Hyun Park (Department of Biochemistry, Chonbuk National University Medical School, Korea). Animal-related experimental procedures were approved by the Institutional Animal Care and Use Committee, Chungnam National University (202012A-CNU-200; Daejeon, Korea), and followed National Institutes of Health guidelines. Animals were housed in sawdust-covered cages in an air-conditioned environment under a 12 h light/dark cycle (5 animals per cage) with free access to standard rodent food and water. Animal breeding was provided by the staffs of IACUC and the Animal Core Facility under the guidance of supervisors who were certified animal technologists. Veterinary care was provided by the IACUC faculty and veterinary residents at the Chungnam National University School of Medicine.

C57BL/6 and BALB/c mice aged 7–8 weeks were purchased from Koatech (Pyeongtaek, Korea) and housed under standard specific pathogen-free conditions at Seoul National University Animal Facility (Seoul, Korea). All animal experiments were performed with the approval of the Institutional Animal Care and Use Committee of Seoul National University Hospital (no.19-0107-S1A1, 25 November 2019).