Seongsanamide A (>98%, Figure 1 ) was isolated from the fermentation broth extracts of Bacillus sp. KCTC 12796BP by as previously reported [7 (link)]. Acetaminophen, amodiaquine, bupropion, chlorzoxazone, dextromethorphan, trimipramine, uridine diphosphoglucuronic acid (UDPGA), nicotinamide adenine dinucleotide phosphate (NADP+), glucose-6-phosphate (G6P), and G6P dehydrogenase (G6PDH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coumarin, midazolam, omeprazole, and tolbutamide were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Pooled HLMs (XTreme 200) were supplied by XenoTech (Lenexa, KS, USA). We purchased rP450 isoforms (rCYP1A2, rCYP2A6, rCYP2B6, rCYP2C8, rCYP2C9, rCYP2C19, rCYP2D6, rCYP2E1, rCYP2J2, rCYP3A4, and rCYP3A5) from Corning life sciences (Woburn, MA, USA). All solvents used in the analyses were LC-MS grade (Fisher Scientific Co., Pittsburgh, PA, USA).
Omeprazole
Manufactured by LGC
Sourced in Canada
Omeprazole is a laboratory reagent used to inhibit gastric acid production. It functions as a proton pump inhibitor, reducing the secretion of stomach acid by blocking the enzyme responsible for acid production. Omeprazole is commonly used in research and development to study gastric acid regulation and related physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Tolbutamide, by LGC (3 mentions)
Formic acid, by Merck Group (2 mentions)
5 hydroxy omeprazole, by LGC (2 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
Chlorzoxazone, by Merck Group (1 mentions)
Dextromethorphan, by Merck Group (1 mentions)
Lc ms grade, by Thermo Fisher Scientific (1 mentions)
Bupropion, by Merck Group (1 mentions)
Trimipramine, by Merck Group (1 mentions)
Midazolam, by LGC (1 mentions)
G6p dehydrogenase g6pdh, by Merck Group (1 mentions)
Coumarin, by LGC (1 mentions)
Pooled hlms xtreme 200, by Xenotech (1 mentions)
Amodiaquine, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Acetonitrile acn, by Merck Group (1 mentions)
Metoprolol, by LGC (1 mentions)
Midazolam, by Tokyo Chemical Industry (1 mentions)
5 protocols using omeprazole
1
Seongsanamide A Biosynthesis and Assay
2
Quantification of Herbal Compounds
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Phenacetin (purity > 99%), omeprazole (purity > 98%), tolbutamide (purity > 98%), and metoprolol (purity > 99%) were purchased from Toronto Research Chemicals Inc. (Toronto, Ontario, Canada). Midazolam (purity > 99%) was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Carbamazepine (IS) was purchased from J&K Chemical. (Shanghai, China); Oldenlandia diffusa (purity > 99%) was purchased from Shanghai Canspec Scientific Instruments Co., Ltd. (Shanghai, China). Formic acid (analytical reagent grade) was purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade methanol and acetonitrile (ACN) were purchased from Merck Company (Darmstadt, Germany). Ultrapure water was produced in a Milli-Q system (Millipore, Bedford, MA, USA). All other chemicals and solvents were at analytical grade level.
3
Investigating Cytochrome P450 Enzymes
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The following are used in the study: testosterone, paracetamol, phenacetin, and NADPH (Beijing Solarbio Science & Technology Co., Ltd. China); dextran, dextromethorphan, omeprazole, 5-hydroxy omeprazole, tolbutamide, 4-hydroxytolbutamide, and 6β-hydroxytestosterone (Toronto Research Chemicals); BCA protein Kit (Lianke Biotechnology Corporate Limited, No. a81911151); primer (synthesized by Tsingke Biotechnology Co., Ltd.); reverse transcription Kit (Tsingke Biotechnology Co., Ltd., No. 20190328); and CYP1A2, cyp2d1, CYP3A4, CYP3A1, CYP2C19, and CYP2C11 antibodies (Abcam Company).
4
Quantitative Bioanalysis of Pharmaceutical Compounds
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Caffeine-d9, ciprofloxacin, efavirenz-d4, 8-hydroxyefavirenz, 8,14-dihydroxyefavirenz, fluconazole, losartan, losartan carboxylic acid (E3174), losartan-d4, α-hydroxyMetoprolol, Metoprolol-d7, omeprazole, 5-hydroxyomeprazole, omeprazole-d3, paroxetine, and rifampicin were purchased from Toronto Research Chemicals (Toronto, Canada). 1’-HydroxyMidazolam and Midazolam-d6 were purchased from Lipomed (Lipomed AG, Arlesheim, Switzerland). Metoprolol, paraxanthine, and β-glucuronidase were obtained from Sigma-Aldrich (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland). Midazolam (Hoffmann-La Roche, Basel, Switzerland) and efavirenz (Merck, NJ, USA) were kindly provided by the producers.
Formic acid, high-performance liquid chromatography (HPLC)-grade methanol, and water were purchased from Merck (Merck, Darmstadt, Germany). Stock solutions, calibration spiking solutions, and quality controls were prepared in dimethyl sulfoxide. Calibration standards were prepared by enriching caffeine-free blank human serum using the corresponding spiking solutions. Internal standard solutions containing the deuterated compounds were prepared in methanol.
Formic acid, high-performance liquid chromatography (HPLC)-grade methanol, and water were purchased from Merck (Merck, Darmstadt, Germany). Stock solutions, calibration spiking solutions, and quality controls were prepared in dimethyl sulfoxide. Calibration standards were prepared by enriching caffeine-free blank human serum using the corresponding spiking solutions. Internal standard solutions containing the deuterated compounds were prepared in methanol.
5
Purification and Characterization of Protein
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Unless otherwise specified, all reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luria-Bertani (LB) medium and isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from AMRESCO LLC (Cochran Road Solon, OH, USA). Glycerol-3-phosphate dehydrogenase (α-GDH) and reduced nicotinamide adenine dinucleotide (NADH) were purchased from Roche (Penzberg, Upper Bavaria, Germany). Immobilized Metal Affinity Chromatography (IMAC) resin was purchased from Bio-Rad (Hercules, California, USA). Amicon Ultra 30 kDa filters were purchased from Millipore Corporation (Billerica, Massachusetts, USA).
The sulfhydryl reagents utilized were methylmethane thiosulfonate (MMTS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) and sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES). The last was purchased from Toronto Research Chemicals (Toronto, Canada).
The commercially approved drugs used were omeprazole, rabeprazole and sulbutiamine. The last was purchased from Santa Cruz, Biotechnology (Dallas, Texas, USA).
The sulfhydryl reagents utilized were methylmethane thiosulfonate (MMTS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) and sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES). The last was purchased from Toronto Research Chemicals (Toronto, Canada).
The commercially approved drugs used were omeprazole, rabeprazole and sulbutiamine. The last was purchased from Santa Cruz, Biotechnology (Dallas, Texas, USA).
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