Anti p65 antibody

Manufactured by Proteintech

The Anti-p65 antibody is a laboratory reagent used for the detection and analysis of the p65 subunit of the NF-κB transcription factor. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of p65 in biological samples.

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Lab products found in correlation

Beyochip enzymatic chip assay kit, by Beyotime (1 mentions)

Close spelling variants of this product

Rabbit anti-p65 antibody Anti-NF-κB-p65 antibody Anti-P65 Anti-TM

2 protocols using anti p65 antibody

Analyzing p65 Expression by IHC

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Immunohistochemistry staining with anti-p65 antibody (Proteintech) was performed according to standard protocols and analyzed under a light microscope.

Chen S., Jiang S., Zheng W., Tu B., Liu S., Ruan H, & Fan C. (2017). RelA/p65 inhibition prevents tendon adhesion by modulating inflammation, cell proliferation, and apoptosis. Cell Death & Disease, 8(3), e2710-.

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ChIP-qPCR Analysis of p65 Binding

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ChIP was performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime) according to the manufacturer’s instructions. An anti-p65 antibody (Proteintech) was employed to immunoprecipitate proteins from cell lysates with A/G Magnetic Beads conjugated to the anti-p65 antibody. Subsequently, qPCR was performed to analyze the enrichment of NUAK2. The primers used for ChIP-qPCR are detailed in Table S1.

Wang R., Su D., Liu Y., Huang H., Qiu J., Cao Z., Yang G., Chen H., Luo W., Tao J., Weng G, & Zhang T. (2024). The NF-κB/NUAK2 signaling axis regulates pancreatic cancer progression by targeting SMAD2/3. iScience, 27(4), 109406.

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