Neutral glue

Manufactured by Solarbio

Sourced in China

Neutral glue is a multi-purpose adhesive designed for laboratory applications. It is a water-based, non-toxic, and odorless formula that forms a strong, flexible bond between a variety of materials. The glue dries clear and can be easily cleaned with water.

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Lab products found in correlation

Hematoxylin, by Proteintech (1 mentions) Affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Ab18256, by Abcam (1 mentions) Nb100 56720, by Novus Biologicals (1 mentions) Pa5 20054, by Thermo Fisher Scientific (1 mentions) Dewaxing solution, by Solarbio (1 mentions) Hematoxylin, by Beyotime (1 mentions) Hydrochloric acid (hcl), by Beyotime (1 mentions) Xylene, by Solarbio (1 mentions) Toluidine blue solution, by Solarbio (1 mentions) Optical microscope, by Olympus (1 mentions) Anhydrous ethanol, by Solarbio (1 mentions)

3 protocols using neutral glue

Quantification of Prostatic Inflammation and Protein Expression

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Prostatic inflammatory infiltrates were graded based on a histopathological classification system (Supplementary Table 1) using H and E staining.11 (link) After being baked, deparaffinized, and rehydrated, prostate tissue sections were stained in hematoxylin (Proteintech Group Inc., Wuhan, China) and eosin-phloxine solution (Servicebio Co., Ltd., Wuhan, China). Then, sections were dehydrated and mounted with neutral glue (Solarbio Co., Ltd., Beijing, China).
The protein expression intensities were identified using IHC staining. After being deparaffinized, rehydrated, antigen retrieved, and blocked, prostate tissue sections were stained with primary antibodies: TLR2 (1:400; NB100-56720, Novus Biologicals Inc., Littleton, CO, USA), TLR10 (1:500; PA5-20054, Thermo Fisher Scientific Co., Ltd., Waltham, MA, USA), high mobility group box 1 (HMGB1; 1:2000; ab18256, Abcam Inc., Cambridge, MA, USA), and biotinylated secondary antibody: AffiniPure Goat anti-Rabbit IgG (Jackson ImmunoResearch Inc., West Grove, PA, USA). Then, sections were dehydrated and mounted with neutral glue. The mean depth of the brown color (three levels) under the microscope (Olympus, Tokyo, Japan) in four random quadrants was regarded as the intensity of relevant protein expression.

Fan Y., Yang L., Wei Q., Ding Y., Tang Z., Tan P., Lin T., Guo D, & Qiu S. (2019). Toll-like receptor 10 (TLR10) exhibits suppressive effects on inflammation of prostate epithelial cells. Asian Journal of Andrology, 21(4), 393-399.

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Histological Analysis of Xenotransplant Tissues

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The tumor, heart, liver, spleen, lung, and kidney from the xenotransplant-model mice were collected. They were then fixed in 4% PFA (Solarbio), paraffin-embedded, sectioned, dewaxed using a dewaxing solution (Solarbio), and rehydrated through a graded alcohol series, as described above. Cell nuclei were stained with hematoxylin (Beyotime) for 3 to 8 min, followed by rinsing the samples with tap water, a brief treatment with 1% hydrochloric acid (Beyotime), and a final rinse with tap water for blue coloration. Cytoplasm staining was achieved by immersing the sections in eosin (Beyotime) for 1 to 3 min. Dehydration was carried out through a graded alcohol series, followed by clearing in a dewaxing solution (Solarbio). Finally, the samples were sealed using neutral glue (Solarbio) and then examined using a light microscope (Leica).

Yu J., Li S., Cheng S., Ahmad M., Chen C., Wan X., Wei S., Pan W, & Luo H. (2024). Tanshinone analog inhibits castration-resistant prostate cancer cell growth by inhibiting glycolysis in an AR-dependent manner. The Journal of Biological Chemistry, 300(4), 107139.

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Nissl Staining of Rat Hippocampal Tissue

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Paraffin‐embedded rat hippocampal tissue sections were dewaxed and stained with 1% toluidine blue solution (Solarbio) for 10 min. Then, after washing with distilled water, Nissl differentiation solution was added dropwise to the tissue sections for 2–4 min. Subsequently, 95% ethanol was added for rapid differentiation, and after the Nissl changed to purple and the other tissues became colorless. The sections were dehydrated with anhydrous ethanol (Solarbio), transparent with xylene (Solarbio), and finally sealed with neutral glue (Solarbio) to seal the sections. Images were captured using an optical microscope (Olympus).

Peng J., He Y., He J., Zhang J., Yu Z, & Xia Y. (2023). GPR30 agonist G1 combined with hypothermia alleviates cognitive impairment and anxiety‐like behavior after subarachnoid hemorrhage in rats. Brain and Behavior, 13(10), e3204.

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