Anti CD133 (ab222782; 1:1000) and anti Nestin (ab134017; 1:500) antibodies were purchased from Abcam (Cambridge, MA). Anti Sox2 clone D6D9 (3579; 1:1000) and anti Oct-4A clone C30A3 (2840; 1:500) antibodies were purchased from Cell Signaling (Danvers, MA). Anti L1CAM (AF277; 1:1000) and anti-NFASC (AF2325; 1:500) were purchased from R&D Systems (Minneapolis, MN). Anti NCAM2 (sc-136328; 1:500) was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti β-actin clone AC-40 (GTX11003; 1:10000) was purchased from Genetex (Irvine, CA). Anti CD11b/c clone OX-42 (201807; 1:100) was purchased from BioLegend (San Diego, CA). Anti CD133 clone TMP4 (46-1338-42: 1:200) antibody, Live/Dead fixable red stain (1:200; L34972) and Live/Dead fixable yellow stain (1:200; L34968) were purchased from Thermo Fisher Scientific. Secondary anti-rabbit IgG HRP-linked (7074; 1:10000) and anti-mouse IgG HRP-linked (7076; 1:10000) antibodies were purchased from Cell Signaling. Recombinant bFGF (233-FB) and EGF (236-EG) were purchased from R&D Systems (Minneapolis, MN).
Anti mouse igg hrp linked 7076
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-mouse IgG HRP-linked (7076) is a secondary antibody conjugated with horseradish peroxidase (HRP) that binds to mouse immunoglobulin G (IgG). It is used for the detection of mouse primary antibodies in immunoassays such as Western blotting and ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Hrp linked anti rabbit igg 7074, by Cell Signaling Technology (2 mentions)
Egf 236 eg, by R&D Systems (1 mentions)
L34968, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Nacalai Tesque (1 mentions)
Wbkls0500, by Merck Group (1 mentions)
P c myc s62, by Cell Signaling Technology (1 mentions)
P p44 42 mapk, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by CoWin Biotech (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Ab193955, by Abcam (1 mentions)
High fat diet (hfd), by Dyets (1 mentions)
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Anti cleaved parp, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti caspase 9, by Cell Signaling Technology (1 mentions)
Anti histone h3, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti phospho pdgfrα, by Cell Signaling Technology (1 mentions)
9 protocols using anti mouse igg hrp linked 7076
1
Stemness Marker Antibody Validation
2
Hypoxia Response in Spalax and Rat Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary Spalax and rat cells (three lines of each species) were exposed to 24 h of normoxia or hypoxia simultaneously with the cells for targeted metabolomics. The cells were lysed by RIPA-buffer on ice-bath immediately after the experiment. The lysates (40 µg protein) were subjected to 10% SDS-PAGE and transferred to a nitrocellulose membrane. For the detection of HIF-1α protein, the anti HIF-1α (28b):sc-13515 (Santa Cruz Biotecnology, Dallas, TX, USA) was used as primary, and anti-mouse IgG, HRP-linked#7076 (Cell Signaling, Danvers, MA, USA), as secondary antibody. The signal intensity was normalized by tubulin and actin probed with anti-Tubulin (10D8; sc-53646) and anti-Actin (I-19; sc-1616) antibodies.
3
Mouse Mammary Epithelial Cell Lysate Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse mammary epithelial cell were lysed by RIPA buffer with protease inhibitor cocktail (25 955; Nacalai Tesque) and the whole‐cell lysate was obtained for IB analysis. Enhanced chemiluminescence reagent (WBKLS0500; Millipore, MA01821) was used to obtain the signals and visualize bands using the ImageQuant LAS4000 chemiluminescent image system. Quantification of the band intensity was undertaken using ImageJ‐NIH software (https://imagej.nih.gov/ij/ ). c‐Myc (9402), P‐c‐Myc‐(S62) (E1J4K) (13 748), p44/42 MAPK (9102), P‐p44/42 MAPK (T202/Y204), GSK‐3β (D5C5Z) XP(R) (12 456), P‐GSK‐3β (S9) (D85E12) (5558) XP(R), P‐Rb(S807/811) (9308), cyclin D1 (2926), PCNA (D3H8P) XP(R) (13 110), anti‐mouse IgG HRP‐linked (7076), and anti‐rabbit IgG HRP‐linked (7074) Abs were purchased from Cell Signaling Technology. Anti‐Myc (phospho‐Thr58) (Y011034), anti‐A‐tubulin mouse MAB (DM1A) and Rb (C‐15) sc:50 Abs were purchased from Applied Biological Materials, EMD Millipore, and Santa Cruz Biotechnology, respectively.
4
Streptozotocin-Induced Rodent Diabetes Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Streptozotocin (STZ) was purchased from Sigma-Aldrich (Catalog #S0130, St.Louis, MO, USA). The high-fat diet (HFD, 60% calories from fat) was purchased from Dyets Inc (Catalog #HF60; Betheleham, PA, USA). ELISA kits for IL-17A, TNF-α, IL-6, and IL-23 were purchased from MEIMIAN (Jiangsu, China). Antibodies against β-actin (#3700S), GAPDH (#5174), anti-rabbit IgG HRP-linked (#7074) and anti-mouse IgG HRP-linked (#7076) antibodies were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). Antibodies against RORγ (#ab113434), IL-17A(#ab193955), and TFF3(#ab272927) were obtained from Abcam (Cambridge, USA). Antibodies against TFF3 Alexa Fluor® 488(#sc-398651 AF488) were purchased from Santa Cruz (Dallas, USA). The bicinchoninic acid (BCA) protein assay kit was purchased from CoWin Biosciences (CW0014, Shanghai, China).
5
Western Blotting Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was performed as described previously.34 (link) The anti-H3K27ac antibody (C15410174) was purchased from Diagenode (Denville, NJ, USA). The anti-PDGFC antibody (#ab93899) and anti-Ki67 (#ab156956) were purchased from Abcam. The anti-PIK3CA antibody (67071-1-Ig) was purchased from Proteintech (Rosement, IL, USA). The anti-β-actin (#4970); anti-histone H3 (#4499); anti-phospho-PDGFRα (#2992); anti-phospho-PDGFRβ (#2227); anti-AKT (#4691); anti-phospho-AKT (#4060); anti-PARP (#9532); anti-cleaved PARP (#5625); anti-caspase3 (#14220S); anti-cleaved caspase3 (#9664S); anti-caspase9 (#9508); anti-cleaved caspase9 (#7237), anti-rabbit immunoglobulin G (IgG), HRP-linked (#7071); and anti-mouse IgG, HRP-linked (#7076) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The dilution ratios of the primary antibodies and the secondary antibodies were 1:1,000 and 1:5,000, respectively.
6
Antibody Detection for Parkin Pathway Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Parkin (4211), cleaved caspase 3 (9664), poly(ADP-ribose) polymerase (PARP) (9542), c-Jun (9165), and voltage-dependent anion channel (VDAC) (4866), phospho-SAPK/JNK (Thr183/Tyr185) (9251), horseradish peroxidase (HRP)-linked anti-mouse IgG (7076) were purchased from Cell Signaling Technology (Danvers, MA, United States). Antibodies against p62 (P0067, Sigma-Aldrich, Saint Louis, MO, United States), LC3B (100-2220, Novus Biologicals, City of Centennial, CO, United States), HRP-conjugated β-actin (47778, Santa Cruz Biotechnology, Dallas, TX, United States) and goat anti-rabbit IgG (H+L) secondary antibody (31460, Thermo Fisher Scientific, Carlsbad, CA, United States), PTEN-induced putative kinase 1 (PINK1) (BC100-494, Novus Biologicals) and ubiquitin phosphorylated at S65 residue (p-Ub-S65) (ABS1513-I, EMD Millipore, Burlington, MA, United States) were purchased at the indicated companies. Bafilomycin A1 (Baf.A1, Sigma-Aldrich), carbonyl cyanide 3-chlorophenylhydrazone (CCCP, Sigma-Aldrich), staurosporine (STS, Cell Signaling Technology), and necrostatin-1 (Invitrogen, Carlsbad, CA, United States) were purchased from the indicated companies.
7
Analyzing K1 Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested, washed twice with PBS, and then lysed in buffer containing 0.5% NP40, 150 mM NaCl, 50 mM Tris-HCL pH 8.0, and a cocktail of proteinase (Roche) and phosphatase (Roche) inhibitors. The lysates used to evaluate K1 protein expression were frozen and thawed two times. Protein concentrations were determined by Bradford assay. Equal amounts of protein (15–25 μg) were loaded per lane and resolved by SDS-PAGE and then transferred to a nitrocellulose membrane. The following antibodies from Cell Signaling Technology were used: AMPKα #2603, AMPKα1 #2795, AMPKα2 #2757, AMPKβ1 #12063, AMPKβ1/2 #4150, AMPKγ1#4187, HRP-linked anti-rabbit IgG #7074 and HRP-linked anti-mouse IgG #7076. In some experiments K1 expression was confirmed by immunoblotting with an HRP-conjugated mouse monoclonal anti-FLAG M2 antibody from Sigma #F1804. The K1 monoclonal antibody was made by immunization with the peptide, KQRDSNKTVP, protein ID#AAB71616 (gene accession #U86667).
8
Acacetin Molecular Targets and Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acacetin (purity 98%) was purchased from Tauto Biotech Co., Ltd. (Shanghai, China) and dissolved in DMSO. Primary antibodies against PI3K p85 (4292), EGFR (4267), Met (8198), GSK3β (9315), MMP9 (13,667), p-EGFR (4404 and 2234), Bax (2774), Bcl-2 (17,447), Bcl-xl (2764), Cleaved Caspase-3 (9661), Caspase-3 (9662), Cleaved PARP (5625), PARP (9532), p-STAT3 (9145), STAT3 (9139), p-ERK (4370), Erk1/2 (4695), PCNA (13,110), and Ki-67 (9027), as well as HRP-linked anti-rabbit IgG (7074) and HRP-linked anti-mouse IgG (7076) were purchased from Cell Signaling Technology (Danvers, MA, United States). Antibodies against PTGS2 (D223097), HAVCR1 (D260086), TMPRSS4 (D123375), GJB4 (D260904), KRT17 (D120232), EGR1 (D120585) and AKR1B10 (D221558) were obtained from Sangon Biotech (Shanghai, China). An antibody against GAPDH (60,004-1) was purchased from Proteintech (Wuhan, China). Human EGF (AF-100-15) was purchased from Peprotech (United States).
9
Western Blot Analysis of Cytoskeletal Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole cell lysates were used for Western blotting following routine protocols for gel electrophoresis and trans-blotting, as described earlier [31, 33] . Equal amounts of 10 µL lysate containing 2 µg/µL protein were loaded on precast TGX stain-free gels (Bio-Rad, Munich, Germany). Transturbo blot PVDF membranes (Bio-Rad) were used for blotting. Each Western blot contained 5 samples for each group: 4 h 1g, 4 h RPM-AD cells, 24 h 1g, 24 h RPM AD cells and 24 h RPM MCS.
The following primary antibodies were used at a dilution of 1:1000: fibronectin (F3648), laminin (L9393) and talin (T3287) (all Sigma-Aldrich) and vascular endothelial growth factor (VEGFA; ab46154, Abcam, Cambridge, United Kingdom). The corresponding secondary antibodies were used at a dilution of 1:3000: HRP-linked anti-mouse IgG (#7076) and anti-rabbit IgG (#7074, both Cell Signaling Technology, Massachusetts, USA). The analysis was performed in ChemiDoc XRS+ (Bio-Rad), and the densitometric quantification was performed using ImageLab (BioRad).
The following primary antibodies were used at a dilution of 1:1000: fibronectin (F3648), laminin (L9393) and talin (T3287) (all Sigma-Aldrich) and vascular endothelial growth factor (VEGFA; ab46154, Abcam, Cambridge, United Kingdom). The corresponding secondary antibodies were used at a dilution of 1:3000: HRP-linked anti-mouse IgG (#7076) and anti-rabbit IgG (#7074, both Cell Signaling Technology, Massachusetts, USA). The analysis was performed in ChemiDoc XRS+ (Bio-Rad), and the densitometric quantification was performed using ImageLab (BioRad).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!